Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   I9192_RS02900 Genome accession   NZ_CP066119
Coordinates   614005..614808 (+) Length   267 a.a.
NCBI ID   WP_004825013.1    Uniprot ID   A0A0A8TUP8
Organism   Acinetobacter bereziniae strain GD03185     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 609005..619808
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I9192_RS02875 (I9192_02875) - 609702..610988 (+) 1287 WP_200150094.1 glycosyltransferase family 1 protein -
  I9192_RS02880 (I9192_02880) - 611019..611570 (+) 552 WP_005033890.1 phosphatase PAP2 family protein -
  I9192_RS02885 (I9192_02885) iscX 611744..611944 (+) 201 WP_004725877.1 Fe-S cluster assembly protein IscX -
  I9192_RS02890 (I9192_02890) ndk 612087..612518 (+) 432 WP_004725878.1 nucleoside-diphosphate kinase -
  I9192_RS02895 (I9192_02895) rlmN 612695..613945 (+) 1251 WP_005033888.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  I9192_RS02900 (I9192_02900) pilF 614005..614808 (+) 804 WP_004825013.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  I9192_RS02905 (I9192_02905) - 614799..615587 (+) 789 WP_004825011.1 RodZ domain-containing protein -
  I9192_RS02910 (I9192_02910) ispG 615609..616724 (+) 1116 WP_004825009.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  I9192_RS02915 (I9192_02915) hisS 616721..618016 (+) 1296 WP_005033885.1 histidine--tRNA ligase -
  I9192_RS02920 (I9192_02920) - 618047..618748 (+) 702 WP_004825005.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 267 a.a.        Molecular weight: 29949.02 Da        Isoelectric Point: 9.2123

>NTDB_id=517476 I9192_RS02900 WP_004825013.1 614005..614808(+) (pilF) [Acinetobacter bereziniae strain GD03185]
MYQKAVVILAIGLSALVVQGCQTTNSDTIIKKDPEKAVKVRTQLAAEYIRSGDLDAAKRALDQALETDSRDSAANMMMGV
LLQQEGSKISMEKADGYFKRAISSDSKNAQARNNYGTYLFVSERYNDAIEQLSIAGASLGYDQRYKALENLGRVYLKVGD
TANAEKAFKNALQVNRDSIISLVELSEIFYLQQKNAQASQLYEQYVRLVGQDKQGARALWIGLRIARANNDQMGMQVLVN
QLRALFPDSKEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 804 bp        

>NTDB_id=517476 I9192_RS02900 WP_004825013.1 614005..614808(+) (pilF) [Acinetobacter bereziniae strain GD03185]
ATGTATCAAAAAGCAGTTGTAATATTGGCAATTGGTTTATCTGCATTGGTGGTACAGGGTTGTCAAACGACAAACTCAGA
CACAATTATAAAAAAAGATCCTGAGAAAGCCGTCAAAGTTCGTACCCAGTTAGCTGCGGAGTATATTCGATCAGGCGATC
TGGATGCAGCCAAACGTGCACTTGATCAAGCCCTTGAAACAGATTCACGAGATTCTGCTGCCAATATGATGATGGGAGTT
TTACTACAACAAGAAGGTAGTAAGATCAGCATGGAAAAAGCGGATGGTTACTTTAAGCGTGCAATTTCCAGTGATTCGAA
AAATGCGCAGGCTCGTAATAATTATGGAACATATTTATTTGTTAGCGAGCGTTATAATGATGCGATTGAGCAGTTGAGTA
TTGCAGGCGCATCTCTTGGATACGACCAGCGTTATAAGGCTTTAGAAAATTTGGGGCGGGTATATTTAAAAGTTGGTGAT
ACAGCAAATGCCGAAAAAGCATTCAAAAATGCCCTACAAGTCAATCGCGATTCGATCATTTCTTTGGTAGAGTTGTCAGA
GATTTTCTATTTGCAACAAAAAAATGCGCAAGCAAGTCAACTCTATGAACAATATGTTCGTTTAGTAGGTCAGGACAAAC
AGGGCGCAAGAGCACTCTGGATTGGTTTACGTATTGCACGTGCCAATAATGACCAGATGGGAATGCAAGTACTGGTGAAC
CAGTTGCGGGCATTATTCCCTGACAGTAAAGAATATCAACGTTATCTGCAATTACAGTACAGTACTGAGGCCGTATGGAA
GTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(116-173)

Tetratricopeptide repeat (TPR_16)

(44-79)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0A8TUP8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

74.717

99.251

0.742
  pilF Acinetobacter baylyi ADP1

67.843

95.506

0.648