Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KZO72_RS01900 Genome accession   NZ_CP080121
Coordinates   414975..415652 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain PK8217     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 409975..420652
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KZO72_RS01885 (KZO72_01890) chiA 411342..414035 (+) 2694 WP_020239449.1 bifunctional chitinase/lysozyme -
  KZO72_RS01890 (KZO72_01895) bfd 414204..414398 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  KZO72_RS01895 (KZO72_01900) bfr 414470..414946 (+) 477 WP_000675504.1 bacterioferritin -
  KZO72_RS01900 (KZO72_01905) pilD 414975..415652 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  KZO72_RS01905 (KZO72_01910) gspM 415652..416113 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  KZO72_RS01910 (KZO72_01915) gspL 416110..417273 (-) 1164 WP_001618895.1 type II secretion system protein GspL -
  KZO72_RS01915 (KZO72_01920) gspK 417288..418271 (-) 984 WP_001058521.1 type II secretion system minor pseudopilin GspK -
  KZO72_RS01920 (KZO72_01925) gspJ 418264..418851 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  KZO72_RS01925 (KZO72_01930) gspI 418844..419221 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  KZO72_RS01930 (KZO72_01935) gspH 419218..419727 (-) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  KZO72_RS01935 (KZO72_01940) gspG 419735..420172 (-) 438 WP_042032455.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=517423 KZO72_RS01900 WP_000178161.1 414975..415652(-) (pilD) [Escherichia coli strain PK8217]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=517423 KZO72_RS01900 WP_000178161.1 414975..415652(-) (pilD) [Escherichia coli strain PK8217]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378