Detailed information NTG0517417

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: KZO72_RS00835
• Genome accession: NZ_CP080121
• Coordinates: 176143..177126 (+)
• Length: 327 a.a.
• NCBI ID: WP_001196486.1
• Uniprot ID: A7ZT85
• Organism: Escherichia coli strain PK8217
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 171143..182126

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KZO72_RS00820 (KZO72_00825)	dppA	172286..173893 (+)	1608	WP_001222883.1	dipeptide ABC transporter substrate-binding protein DppA	-
KZO72_RS00825 (KZO72_00830)	dppB	174201..175220 (+)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
KZO72_RS00830 (KZO72_00835)	dppC	175230..176132 (+)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
KZO72_RS00835 (KZO72_00840)	amiE	176143..177126 (+)	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	Regulator
KZO72_RS00840 (KZO72_00845)	dppF	177123..178127 (+)	1005	WP_000107012.1	dipeptide ABC transporter ATP-binding subunit DppF	-
KZO72_RS00845 (KZO72_00850)	yhjV	178157..179428 (-)	1272	WP_001295225.1	aromatic amino acid transport family protein	-
KZO72_RS00850 (KZO72_00855)	ldrD	179904..180011 (+)	108	WP_000141634.1	type I toxin-antitoxin system toxic polypeptide LdrD	-
KZO72_RS00855 (KZO72_00860)	bcsG	180098..181777 (-)	1680	WP_074524343.1	cellulose biosynthesis protein BcsG	-
KZO72_RS00860 (KZO72_00865)	bcsF	181774..181965 (-)	192	WP_000988308.1	cellulose biosynthesis protein BcsF	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35844.36 Da
• Isoelectric Point: 6.5814

>NTDB_id=517417 KZO72_RS00835 WP_001196486.1 176143..177126(+) (amiE) [Escherichia coli strain PK8217]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=517417 KZO72_RS00835 WP_001196486.1 176143..177126(+) (amiE) [Escherichia coli strain PK8217]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCGCCGTTCCGCGCCGTAGACCGCATCAG
CTACAGCGTAAAACAGGGCGAAGTGGTCGGGATTGTGGGTGAGTCCGGCTCCGGTAAGTCGGTCAGTTCACTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTAATGGCAGAAAAACTGGAGTTTAACGGCCAGGATTTGCAGCGTATCTCAGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAGGGCGGCAACAAAAGTACCCGCCGTCAGCGAGCGATTGACC
TGCTGAATCAGGTCGGTATTCCCGATCCGGCATCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATCGCCATGGCGATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCGACCACCGCGCTGGACGTGAC
CATTCAGGCGCAAATCATCGAACTACTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATCATCGTGATGTATGCAGGCCAGGTGGTGGAAACCGGTGATGCGCACGCC
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCAGAATTTGCTCAGGACAAAGAACGTCT
GGCGTCGTTGCCAGGTGTCGTTCCCGGCAAGTACGACCGCCCGAACGGCTGCCTGCTTAACCCGCGCTGCCCCTATGCCA
CTGACAGATGTCGCGCTGAAGAACCGGCGCTGAATATGCTCGCTGACGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

• ABC transporter (ABC_tran) (24-181)

3D structure

• AlphaFold DB: A7ZT85

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404