Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   I6I06_RS09745 Genome accession   NZ_CP066075
Coordinates   2232227..2233744 (+) Length   505 a.a.
NCBI ID   WP_042328815.1    Uniprot ID   A0A7T4MZQ5
Organism   Paraburkholderia ginsengisoli strain FDAARGOS_1049     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2227227..2238744
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6I06_RS09720 (I6I06_09720) kdsD 2228421..2229404 (+) 984 WP_042328823.1 arabinose 5-phosphate isomerase KdsD -
  I6I06_RS09725 (I6I06_09725) - 2229404..2229964 (+) 561 WP_042328821.1 HAD family hydrolase -
  I6I06_RS09730 (I6I06_09730) lptC 2229961..2230560 (+) 600 WP_042328819.1 LPS export ABC transporter periplasmic protein LptC -
  I6I06_RS09735 (I6I06_09735) lptA 2230590..2231261 (+) 672 WP_042328817.1 lipopolysaccharide transport periplasmic protein LptA -
  I6I06_RS09740 (I6I06_09740) lptB 2231258..2232043 (+) 786 WP_042328816.1 LPS export ABC transporter ATP-binding protein -
  I6I06_RS09745 (I6I06_09745) rpoN/rpoN1 2232227..2233744 (+) 1518 WP_042328815.1 RNA polymerase factor sigma-54 Machinery gene
  I6I06_RS09750 (I6I06_09750) hpf 2233915..2234271 (+) 357 WP_042328814.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  I6I06_RS09755 (I6I06_09755) - 2234549..2235064 (+) 516 WP_084585490.1 PTS sugar transporter subunit IIA -
  I6I06_RS09760 (I6I06_09760) hprK 2235177..2236145 (+) 969 WP_012431656.1 HPr(Ser) kinase/phosphatase -
  I6I06_RS09765 (I6I06_09765) rapZ 2236243..2237136 (+) 894 WP_042328840.1 RNase adapter RapZ -
  I6I06_RS09770 (I6I06_09770) - 2237175..2237810 (+) 636 WP_042328812.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 505 a.a.        Molecular weight: 55531.55 Da        Isoelectric Point: 4.9946

>NTDB_id=517217 I6I06_RS09745 WP_042328815.1 2232227..2233744(+) (rpoN/rpoN1) [Paraburkholderia ginsengisoli strain FDAARGOS_1049]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAISQNPLLENEDDWIASPLRVAADGSLIAQAPNSSAPSDQM
GGNGSSSSTSSSERSENGEPQGVDEYNGMSGDSNGDASQWNLDDYGRSGNASDDDDLPPLQIHESSTSLRDHLMAQLRVT
QAGQRDRALITFLIESLDDDGYLAATLDEVQADLPEELEVDLDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSP
TRTLALDIVSHYLELLAARDFTRLRKHLKANDDDLRDAHMLIRSLEPFPGAAYGKAEADYVVPDIMVRKTAQGWQAELNP
EVVPKLRINHLYANILRNNRGDPGSGSLRQQLQEARWLIKNIQQRFETILRVAQAIVERQKSFFVHGEIAMRPLVLREIA
DTLGLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAENPKSPLSDSRIAELLAEQGF
VVARRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1518 bp        

>NTDB_id=517217 I6I06_RS09745 WP_042328815.1 2232227..2233744(+) (rpoN/rpoN1) [Paraburkholderia ginsengisoli strain FDAARGOS_1049]
ATGAAAGCCAGCCTCCAACTCCGCCTATCGCAGCATCTTGCGCTGACGCCCCAACTGCAGCAGTCCATCCGGCTGCTCCA
GTTGTCCACGCTCGAACTGCAGCAGGAAGTCGCAATGGCGATCTCGCAGAATCCGCTCCTCGAAAATGAGGACGACTGGA
TCGCGAGCCCGTTGCGGGTAGCGGCTGACGGTTCGCTGATCGCCCAGGCGCCCAACTCGTCGGCGCCGTCCGACCAGATG
GGCGGCAACGGCTCGTCTTCGTCCACCAGCAGCAGCGAGCGCTCCGAAAACGGCGAGCCGCAAGGTGTCGACGAATACAA
CGGCATGTCGGGCGATAGCAACGGCGACGCGTCGCAATGGAATCTCGACGACTACGGCCGCTCCGGCAACGCGTCCGACG
ACGACGATCTGCCGCCGCTGCAAATCCACGAATCGAGCACGTCGCTGCGTGATCATCTGATGGCGCAATTGCGCGTCACC
CAGGCGGGTCAACGCGACCGGGCGCTGATCACGTTCCTGATCGAATCGCTCGACGACGATGGCTATCTCGCCGCGACGCT
CGACGAAGTGCAAGCCGATCTGCCCGAAGAGCTCGAAGTCGATCTCGACGAATTGAACGCGGCGCTCGCGTTGCTGCATA
GCTTCGACCCGGCGGGGGTGGGCGCGCGTTCCGCATCCGAGTGTCTGAAGCTGCAACTGCTGCGGCTCGATCCTTCGCCT
ACGCGAACGCTCGCACTCGACATCGTCAGTCACTACCTCGAACTGCTCGCGGCACGCGATTTCACGCGTTTGCGCAAACA
CCTGAAGGCGAACGACGACGATCTGCGCGATGCGCACATGCTGATCCGCTCGCTCGAACCGTTTCCCGGCGCCGCCTACG
GCAAGGCCGAAGCGGACTATGTGGTGCCGGACATCATGGTGCGCAAAACGGCCCAGGGCTGGCAGGCGGAACTGAATCCG
GAGGTGGTGCCGAAGCTGCGCATCAACCACTTGTACGCGAATATTCTGCGCAATAACCGGGGCGATCCGGGGAGCGGTTC
GTTGCGCCAGCAACTGCAGGAAGCACGCTGGCTGATCAAGAATATCCAGCAGCGTTTCGAGACGATCCTCCGTGTCGCGC
AAGCCATTGTCGAGCGTCAAAAGAGCTTTTTCGTGCACGGCGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATTGCT
GATACGCTGGGCCTACACGAGTCGACTGTCTCGCGTGTGACAACCGGTAAATACATGCTGACCCCATTCGGGACGCTGGA
ATTTAAGTACTTTTTCGGATCACACGTTTCGACGGACACAGGCGGCGCGGCCTCTTCCACGGCCATTCGCGCGCTGATCA
AACAACTGATAGGAGCGGAAAACCCGAAATCGCCTCTCTCAGACAGTCGCATAGCCGAACTGCTGGCGGAACAGGGCTTC
GTGGTCGCACGGCGTACCGTTGCGAAGTACCGCGAAGCGCTCAAAATCCCGGCAGTCAACCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(141-332)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(348-503)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7T4MZQ5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

66.601

100

0.671