Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   I6H84_RS00455 Genome accession   NZ_CP066037
Coordinates   95510..97015 (-) Length   501 a.a.
NCBI ID   WP_011657965.1    Uniprot ID   Q0BBR6
Organism   Burkholderia ambifaria strain FDAARGOS_1027     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 90510..102015
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6H84_RS00430 (I6H84_00430) - 91540..92175 (-) 636 WP_006752630.1 LON peptidase substrate-binding domain-containing protein -
  I6H84_RS00435 (I6H84_00435) rapZ 92202..93110 (-) 909 WP_041489859.1 RNase adapter RapZ -
  I6H84_RS00440 (I6H84_00440) hprK 93186..94154 (-) 969 WP_011657967.1 HPr(Ser) kinase/phosphatase -
  I6H84_RS00445 (I6H84_00445) - 94284..94823 (-) 540 WP_011657966.1 PTS sugar transporter subunit IIA -
  I6H84_RS00450 (I6H84_00450) hpf 94993..95352 (-) 360 WP_006477786.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  I6H84_RS00455 (I6H84_00455) rpoN/rpoN1 95510..97015 (-) 1506 WP_011657965.1 RNA polymerase factor sigma-54 Machinery gene
  I6H84_RS00460 (I6H84_00460) lptB 97186..97962 (-) 777 WP_011657964.1 LPS export ABC transporter ATP-binding protein -
  I6H84_RS00465 (I6H84_00465) lptA 97959..98624 (-) 666 WP_011657963.1 lipopolysaccharide transport periplasmic protein LptA -
  I6H84_RS00470 (I6H84_00470) lptC 98645..99259 (-) 615 WP_011657962.1 LPS export ABC transporter periplasmic protein LptC -
  I6H84_RS00475 (I6H84_00475) - 99263..99799 (-) 537 WP_011657961.1 KdsC family phosphatase -
  I6H84_RS00480 (I6H84_00480) kdsD 99799..100782 (-) 984 WP_011657960.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54763.85 Da        Isoelectric Point: 4.8795

>NTDB_id=516601 I6H84_RS00455 WP_011657965.1 95510..97015(-) (rpoN/rpoN1) [Burkholderia ambifaria strain FDAARGOS_1027]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENEDEWITSPLRVASDGSLIAQGPSGQNIEPGP
ANGSSQSSERAERDEPAGADEYDGYGSGDAGDGPQWNLDEYGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQASP
RDRALVMFLIESLDDDGYLTASLDEVLSDLPPELEVDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDASPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVMP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=516601 I6H84_RS00455 WP_011657965.1 95510..97015(-) (rpoN/rpoN1) [Burkholderia ambifaria strain FDAARGOS_1027]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACACCTGGCGCTGACCCCGCAACTGCAGCAGTCGATCCGGCTCCTGCA
ACTGTCGACGCTCGAACTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAAAATCCGTTGCTCGAAAACGAAGACGAATGGA
TCACGAGTCCGCTGCGTGTCGCGTCGGACGGATCGCTGATCGCGCAGGGGCCTTCCGGACAGAACATCGAGCCGGGCCCC
GCGAACGGCAGCAGCCAGTCGAGCGAGCGCGCCGAGCGCGACGAGCCGGCCGGCGCCGACGAATACGACGGCTACGGGTC
GGGCGACGCCGGCGACGGCCCGCAGTGGAATCTGGACGAGTACGGCCGCTCGAGCGGCGCATCGGACGATGACGACCTCC
CGCCGCTGCAGGTGCAGGAAGCCGCGACGTCGCTGCGCGACCACCTTTCCGCCCAATTGCGCGTCACGCAGGCCAGCCCG
CGCGATCGCGCACTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCGTCGCTCGACGAAGTACT
CTCCGACCTGCCGCCGGAACTGGAAGTCGACTGCGACGAACTGAATGCGGCGCTCGCGCTGCTGCACAGTTTCGACCCGG
CCGGGGTCGGGGCCCGCTCGGCGTCCGAATGCCTGAAGCTGCAGTTGCTGCGCCTCGATGCGTCCCCCACCCGCACGCTC
GCGCTGGAGATCGTGTCGCAGCACCTCGAGCTGCTCGCCGCGCGCGACTTCACGCGGCTGCGCAAGCACCTGAAGGCGAG
CGACGATGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTCGAGCCGTTCCCAGGCGCCGCGTACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGCTGGCTCGCGGAACTCAATCCGGAAGTGATGCCG
CGCCTGCGGATCAACAACCTGTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGGTCGCTCAAGCAGCA
GCTCCAGGAAGCACGCTGGCTGATCAAGAACATCCAGCAGCGATTCGACACGATCCTGCGTGTCGCGCAGGCCATTGTCG
AACGTCAAAAGAATTTCTTCGCGCATGGTGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACAGGCAAGTACATGCTGACCCCGTTCGGTACGCTGGAATTCAAGTACTT
CTTCGGGTCACACGTTTCGACCGACACCGGAGGCGCCGCATCGTCGACCGCCATCCGAGCCCTGATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGTTTCGTGGTTGCGCGC
CGCACGGTTGCCAAGTATCGCGAAGCCCTCAAAATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q0BBR6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.515

100

0.691