Detailed information NTG0516600

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: I6H84_RS00360
• Genome accession: NZ_CP066037
• Coordinates: 79150..80310 (-)
• Length: 386 a.a.
• NCBI ID: WP_011657978.1
• Uniprot ID: Q0BBP8
• Organism: Burkholderia ambifaria strain FDAARGOS_1027
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 74150..85310

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6H84_RS00340 (I6H84_00340)	-	74668..75051 (+)	384	WP_011657982.1	nuclear transport factor 2 family protein	-
I6H84_RS00345 (I6H84_00345)	leuA	75195..76841 (-)	1647	WP_011657981.1	2-isopropylmalate synthase	-
I6H84_RS00350 (I6H84_00350)	ybiB	77232..78206 (-)	975	WP_041491401.1	DNA-binding protein YbiB	-
I6H84_RS00355 (I6H84_00355)	maiA	78396..79040 (+)	645	WP_041491400.1	maleylacetoacetate isomerase	-
I6H84_RS00360 (I6H84_00360)	pilA	79150..80310 (-)	1161	WP_011657978.1	signal recognition particle-docking protein FtsY	Machinery gene
I6H84_RS00365 (I6H84_00365)	rsmD	80512..81126 (+)	615	WP_041491281.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
I6H84_RS00370 (I6H84_00370)	coaD	81238..81735 (+)	498	WP_006398238.1	pantetheine-phosphate adenylyltransferase	-
I6H84_RS00375 (I6H84_00375)	-	81795..82061 (+)	267	WP_011657976.1	YfhL family 4Fe-4S dicluster ferredoxin	-
I6H84_RS00380 (I6H84_00380)	hisC	82166..83233 (-)	1068	WP_011657975.1	histidinol-phosphate transaminase	-
I6H84_RS00385 (I6H84_00385)	pth	83359..83958 (-)	600	WP_006760396.1	aminoacyl-tRNA hydrolase	-
I6H84_RS00390 (I6H84_00390)	-	84088..84696 (-)	609	WP_006753282.1	50S ribosomal protein L25/general stress protein Ctc	-

Sequence

Protein

• Length: 386 a.a.
• Molecular weight: 41170.99 Da
• Isoelectric Point: 4.8019

>NTDB_id=516600 I6H84_RS00360 WP_011657978.1 79150..80310(-) (pilA) [Burkholderia ambifaria strain FDAARGOS_1027]
MFSFFKRFKKTQESDSTESQSADALQPDEPSDAPAADARQAIEAPPAPAQPQPAAPAVVMTVTPTNDGSDDVVEAVEIVP
PPTQDASAKKSWLTRLKTGLAKTGSSITGVFVNTKIDEDLYEELEAALLMSDAGVDATEYLLGALREKVKTGRLTDPQQV
KSALHDLLVELLKPLEKSLMLGRAQPLVMMITGVNGAGKTTSIGKLAKHLQSFDQSVLLAAGDTFRAAAREQLAVWGERN
NVTVVQQESGDPAAVIFDAVSAARARKIDVMMADTAGRLPTQLHLMEELKKVKRVISKAHDGAPHEVLLVIDANTGQNAL
TQVKAFDDALGLTGLIVTKLDGTAKGGILAAIARQRPVPVYFIGVGEKVEDLQPFNAVEFADALFD

Nucleotide

• Length: 1161 bp

>NTDB_id=516600 I6H84_RS00360 WP_011657978.1 79150..80310(-) (pilA) [Burkholderia ambifaria strain FDAARGOS_1027]
ATGTTCAGCTTCTTCAAACGATTCAAGAAAACGCAGGAGTCCGACTCCACGGAATCGCAATCGGCCGACGCGCTGCAACC
GGACGAGCCGTCCGATGCGCCCGCCGCCGATGCCCGTCAGGCGATCGAAGCCCCGCCAGCCCCCGCGCAACCGCAACCGG
CCGCGCCGGCCGTCGTGATGACGGTCACGCCGACCAACGACGGCAGCGACGACGTCGTCGAAGCGGTCGAGATCGTGCCG
CCGCCGACGCAGGACGCCTCCGCGAAGAAATCGTGGCTCACCCGCCTGAAAACGGGGCTCGCGAAGACGGGTTCGAGCAT
CACCGGCGTGTTCGTCAACACGAAGATCGACGAGGATCTGTACGAAGAACTCGAAGCCGCGCTGCTGATGTCCGACGCCG
GCGTCGATGCGACCGAGTACCTGCTCGGTGCGCTGCGCGAGAAAGTGAAAACGGGCCGGCTGACCGATCCGCAGCAGGTG
AAGAGCGCGCTGCACGATCTGCTCGTCGAACTGCTGAAGCCGCTCGAGAAATCGCTGATGCTCGGCCGCGCGCAGCCGCT
CGTGATGATGATCACGGGCGTGAACGGCGCGGGCAAGACCACCAGCATCGGCAAGCTCGCGAAGCATCTGCAGAGCTTCG
ACCAATCGGTGCTGCTGGCCGCGGGCGACACGTTCCGCGCGGCCGCGCGCGAACAGCTGGCGGTCTGGGGCGAGCGCAAC
AACGTGACGGTCGTCCAGCAGGAAAGCGGCGATCCGGCCGCGGTGATCTTCGACGCGGTCAGCGCCGCGCGCGCGCGCAA
GATCGACGTGATGATGGCCGACACGGCCGGCCGCCTGCCGACGCAGCTGCACCTGATGGAAGAGCTGAAGAAGGTGAAGC
GCGTGATCTCGAAGGCGCACGACGGCGCGCCGCACGAGGTGCTGCTCGTGATCGACGCGAACACCGGCCAGAACGCGCTC
ACGCAGGTGAAGGCCTTCGACGATGCGCTCGGCCTCACGGGCCTGATCGTCACGAAGCTCGACGGCACCGCCAAGGGCGG
GATTCTGGCGGCGATCGCGCGGCAGCGCCCGGTGCCCGTCTACTTCATCGGCGTCGGCGAGAAGGTCGAGGACCTGCAGC
CGTTCAACGCCGTGGAATTCGCGGACGCGCTATTCGACTGA

Domains

Predicted by InterproScan.

• SRP54-type protein, GTPase domain (SRP54) (187-385)

• SRP54-type protein, helical bundle domain (SRP54_N) (104-166)

3D structure

• AlphaFold DB: Q0BBP8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	52.632	100	0.57