Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   KZO13_RS18550 Genome accession   NZ_CP080056
Coordinates   3892385..3892825 (+) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain 18SZHZ655Rt     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3887385..3897825
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KZO13_RS18530 (KZO13_18525) aroP 3888383..3889756 (+) 1374 WP_001331235.1 aromatic amino acid transporter AroP -
  KZO13_RS18535 (KZO13_18530) ampE 3889799..3890653 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  KZO13_RS18540 (KZO13_18535) ampD 3890650..3891201 (-) 552 WP_001402016.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  KZO13_RS18545 (KZO13_18540) nadC 3891289..3892182 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  KZO13_RS18550 (KZO13_18545) pilA 3892385..3892825 (+) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  KZO13_RS18555 (KZO13_18550) pilB 3892835..3894220 (+) 1386 WP_001402015.1 type II secretion system protein GspE Machinery gene
  KZO13_RS18560 (KZO13_18555) hofC 3894210..3895412 (+) 1203 WP_000157236.1 protein transport protein HofC -
  KZO13_RS18565 (KZO13_18560) guaC 3895447..3896490 (-) 1044 WP_001217338.1 GMP reductase -
  KZO13_RS18570 (KZO13_18565) - 3896646..3896690 (-) 45 WP_120795372.1 protein YacM -
  KZO13_RS18575 (KZO13_18570) coaE 3896715..3897335 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=516564 KZO13_RS18550 WP_000360895.1 3892385..3892825(+) (pilA) [Escherichia coli strain 18SZHZ655Rt]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=516564 KZO13_RS18550 WP_000360895.1 3892385..3892825(+) (pilA) [Escherichia coli strain 18SZHZ655Rt]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363