Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   I6H87_RS05225 Genome accession   NZ_CP066018
Coordinates   1132763..1133923 (+) Length   386 a.a.
NCBI ID   WP_011614476.1    Uniprot ID   Q0KEQ7
Organism   Cupriavidus necator strain FDAARGOS_1030     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1127763..1138923
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6H87_RS05200 (I6H87_05200) - 1127775..1128035 (-) 261 WP_010814370.1 YfhL family 4Fe-4S dicluster ferredoxin -
  I6H87_RS05205 (I6H87_05205) coaD 1128104..1128589 (-) 486 WP_010814369.1 pantetheine-phosphate adenylyltransferase -
  I6H87_RS05210 (I6H87_05210) rsmD 1128830..1129522 (-) 693 WP_011614477.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  I6H87_RS05215 (I6H87_05215) - 1129605..1130987 (-) 1383 WP_010814798.1 M16 family metallopeptidase -
  I6H87_RS05220 (I6H87_05220) - 1130997..1132544 (-) 1548 WP_010814799.1 M16 family metallopeptidase -
  I6H87_RS05225 (I6H87_05225) pilA 1132763..1133923 (+) 1161 WP_011614476.1 signal recognition particle-docking protein FtsY Machinery gene
  I6H87_RS05230 (I6H87_05230) maiA 1133941..1134588 (-) 648 WP_010814801.1 maleylacetoacetate isomerase -
  I6H87_RS05235 (I6H87_05235) - 1134593..1135294 (-) 702 WP_011614475.1 fumarylacetoacetate hydrolase family protein -
  I6H87_RS05240 (I6H87_05240) - 1135548..1136195 (+) 648 WP_037025353.1 ANTAR domain-containing response regulator -
  I6H87_RS05245 (I6H87_05245) - 1136561..1137871 (+) 1311 WP_010814804.1 CmpA/NrtA family ABC transporter substrate-binding protein -
  I6H87_RS05250 (I6H87_05250) ntrB 1137923..1138828 (+) 906 WP_010814805.1 nitrate ABC transporter permease -

Sequence

Protein


Download         Length: 386 a.a.        Molecular weight: 40868.63 Da        Isoelectric Point: 7.3625

>NTDB_id=516413 I6H87_RS05225 WP_011614476.1 1132763..1133923(+) (pilA) [Cupriavidus necator strain FDAARGOS_1030]
MFSFWKKRKAEPAPAEAPAPAPAPVEVPAPVPAPAPTPAPVPAPAPAPVPAQVPPPAPAPVAAPIPLPAPAPAEALELVP
PPAQTAEAKLGWMHRLRTGLSKTSRNIGTLFVGVKVDEALFEELETALLMADAGVEATEYLLGELRKRVKAERIETAEGV
KAALRELLTQLLRPLEKTMALGREQPLVMMIAGVNGAGKTTSIGKLCKHFQRYDQKVLLAAGDTFRAAAREQLTIWGERN
NVTVVAQESGDPAAVIFDAVNAAKARGIDIVMADTAGRLPTQLHLMEELKKVKRVISKAMPSAPHEVLLVIDANTGQNAL
QQTRAFDDALGLTGLIVTKLDGTAKGGILAAIARQRPVPVYFIGVGEKVEDLQPFKADEFAEALLG

Nucleotide


Download         Length: 1161 bp        

>NTDB_id=516413 I6H87_RS05225 WP_011614476.1 1132763..1133923(+) (pilA) [Cupriavidus necator strain FDAARGOS_1030]
ATGTTTAGTTTCTGGAAGAAGCGCAAGGCCGAGCCCGCGCCCGCCGAGGCGCCAGCCCCGGCCCCCGCTCCGGTCGAGGT
CCCCGCCCCCGTACCGGCACCGGCCCCCACGCCTGCGCCAGTCCCTGCGCCCGCACCGGCACCGGTCCCGGCGCAAGTGC
CACCCCCTGCCCCAGCGCCCGTGGCGGCCCCGATCCCCCTGCCGGCGCCCGCACCGGCCGAAGCGCTCGAGCTGGTCCCG
CCGCCCGCCCAGACCGCTGAAGCGAAGCTGGGCTGGATGCATCGGCTGCGTACCGGGCTGTCCAAGACCAGCCGCAACAT
CGGCACGCTGTTCGTCGGCGTCAAGGTCGACGAGGCCCTGTTCGAAGAACTGGAAACCGCGCTGCTGATGGCAGATGCCG
GCGTGGAAGCCACCGAGTACCTGCTGGGCGAGTTGCGCAAGCGCGTCAAGGCCGAGCGCATCGAGACCGCCGAAGGCGTC
AAGGCGGCGCTGCGCGAGCTGCTGACGCAGCTGCTGCGCCCGCTGGAAAAAACCATGGCGCTGGGCCGCGAGCAGCCGCT
GGTGATGATGATCGCGGGCGTCAACGGCGCCGGCAAGACCACCAGCATCGGCAAGCTGTGCAAGCACTTCCAGCGCTATG
ACCAGAAGGTGCTGCTGGCCGCCGGCGATACCTTCCGCGCCGCCGCGCGCGAGCAACTGACGATCTGGGGCGAACGCAAC
AACGTCACCGTGGTGGCGCAGGAAAGCGGCGACCCGGCGGCGGTGATCTTCGACGCCGTCAATGCGGCCAAGGCGCGCGG
CATCGACATCGTCATGGCCGACACCGCCGGCCGCCTGCCGACGCAGCTGCACCTGATGGAAGAGCTCAAGAAGGTCAAGC
GCGTGATCAGCAAGGCCATGCCGAGCGCGCCGCACGAGGTGCTGCTGGTGATCGACGCCAACACCGGCCAGAACGCCCTG
CAGCAAACCCGCGCCTTCGACGACGCGCTGGGCCTGACCGGCCTGATCGTGACCAAGCTCGACGGCACCGCCAAGGGCGG
CATCCTGGCGGCTATCGCGCGCCAGCGCCCGGTGCCGGTTTATTTCATTGGCGTGGGCGAGAAGGTGGAGGACCTGCAGC
CGTTCAAGGCGGATGAGTTCGCCGAAGCGCTGCTGGGGTAA

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(187-386)

SRP54-type protein, helical bundle domain (SRP54_N)

(111-166)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q0KEQ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

51.202

100

0.552