Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   THIVI_RS01175 Genome accession   NC_018012
Coordinates   251519..253234 (-) Length   571 a.a.
NCBI ID   WP_014776819.1    Uniprot ID   I3Y5P3
Organism   Thiocystis violascens DSM 198     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 246519..258234
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  THIVI_RS01155 (Thivi_0238) - 248285..248554 (-) 270 WP_014776815.1 hypothetical protein -
  THIVI_RS01160 (Thivi_0239) coaE 248648..249247 (-) 600 WP_014776816.1 dephospho-CoA kinase -
  THIVI_RS01165 (Thivi_0240) pilD 249334..250236 (-) 903 WP_014776817.1 A24 family peptidase Machinery gene
  THIVI_RS01170 (Thivi_0241) pilC 250242..251498 (-) 1257 WP_014776818.1 type II secretion system F family protein Machinery gene
  THIVI_RS01175 (Thivi_0242) pilB 251519..253234 (-) 1716 WP_014776819.1 type IV-A pilus assembly ATPase PilB Machinery gene
  THIVI_RS01180 (Thivi_0243) - 253248..254081 (-) 834 WP_014776820.1 NYN domain-containing protein -
  THIVI_RS01185 (Thivi_0244) acs 254349..256295 (-) 1947 WP_014776821.1 acetate--CoA ligase -
  THIVI_RS01190 (Thivi_0245) - 256560..257237 (-) 678 WP_014776822.1 exonuclease domain-containing protein -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63368.84 Da        Isoelectric Point: 6.3396

>NTDB_id=51640 THIVI_RS01175 WP_014776819.1 251519..253234(-) (pilB) [Thiocystis violascens DSM 198]
MSKDQATIKPSGLAWRLVKDHLLTEQQMMTAYEESTRQGRAFVRHLIEQKLLDSRRIAVAASHEFGVPLLDLGAFDLACL
PLNLVDERLIRKHRALPLLRRGNRLFLALSDPTNDQALEEIRFNTGLSTSAILVEEDRLNVAIETAIAAQDTTTISEFVD
GDLEKLDIAPEDDEQRDESEANIDDAPIVRYVNKILVDAITAGASDIHFEPFEKFFRIRFRQDGMLREVASPPLNTAGRL
TARLKVMSRMNIAERRVPQDGRIRLKLSRSRDIDFRVNTLPTLYGEKVVLRILDSSSAQVGIEALGFEPEQREAFLGAIK
KPYGMILVTGPTGSGKTVSLYTALNLLNQPEINISTVEDPVEIQVAGINQVNLNPKTGLTFASALRAFLRQDPDILMVGE
IRDLETAEIAVKAAQTGHLVLSTLHTNDAPQTLTRLANMGIPPFNIASSVLLIMAQRLARRLCVHCRKKLDLPREALREE
GFSNEEIDTGLTIYGPVGCEKCNKGYKGRVGIFQVMKVTDAITRLILQGGDAMQLADQAQREGVSDLRQSGLRKVKAGLI
GLEELNRVTKD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=51640 THIVI_RS01175 WP_014776819.1 251519..253234(-) (pilB) [Thiocystis violascens DSM 198]
ATGTCAAAGGATCAAGCCACGATCAAACCCTCGGGACTGGCCTGGCGACTGGTCAAGGATCATCTGCTGACCGAACAGCA
GATGATGACCGCCTATGAGGAATCGACCCGTCAGGGACGCGCCTTCGTGCGCCATCTGATCGAACAGAAGCTGCTGGACA
GCCGGCGCATCGCGGTGGCTGCCTCGCATGAGTTCGGCGTCCCGCTGCTCGATCTCGGCGCCTTTGACCTCGCCTGTCTT
CCCCTGAATCTGGTCGACGAACGGCTGATCCGCAAGCATCGCGCACTGCCGCTGCTGCGCCGGGGCAACCGTCTTTTCCT
TGCGCTCTCCGACCCCACCAACGACCAGGCGCTGGAGGAAATCCGTTTCAATACTGGACTCTCGACCAGCGCGATCCTGG
TGGAAGAGGATCGGCTCAACGTAGCCATCGAAACCGCGATCGCCGCGCAAGACACCACGACCATCAGCGAATTCGTCGAT
GGGGATCTGGAAAAGCTCGACATCGCGCCGGAGGATGACGAGCAGCGCGACGAATCCGAAGCCAACATCGACGACGCGCC
CATCGTGCGTTATGTCAACAAAATCCTCGTGGACGCCATCACGGCCGGTGCCTCCGATATTCATTTCGAGCCCTTCGAGA
AATTTTTCCGGATCCGCTTCCGGCAGGATGGCATGCTGCGCGAGGTCGCCAGCCCTCCGCTAAATACCGCCGGACGTCTG
ACCGCGCGTCTCAAGGTCATGTCGCGCATGAACATCGCCGAACGGCGCGTGCCCCAGGATGGGCGCATCCGGCTGAAGTT
GAGCCGTTCGCGCGACATCGATTTCCGTGTCAATACGCTGCCGACGCTCTATGGCGAAAAGGTCGTACTGCGGATCCTGG
ATTCCTCCTCGGCGCAGGTCGGCATCGAGGCGCTCGGCTTCGAACCCGAACAACGTGAGGCATTTCTCGGCGCGATCAAG
AAACCCTATGGCATGATCCTGGTGACTGGCCCCACCGGGTCCGGCAAGACGGTCTCGCTCTATACGGCGCTCAATCTCTT
GAATCAGCCCGAAATCAACATCTCCACCGTCGAGGATCCGGTCGAAATCCAGGTGGCCGGCATCAATCAGGTCAACCTGA
ATCCCAAGACCGGGCTCACCTTCGCGTCCGCCCTGCGCGCCTTTCTGCGCCAAGATCCGGATATCCTCATGGTCGGCGAG
ATCCGCGACCTGGAAACCGCCGAGATCGCGGTGAAGGCCGCACAGACCGGACATCTGGTGCTCTCCACCCTGCATACCAA
CGACGCGCCGCAAACCCTGACCCGTCTGGCCAACATGGGGATCCCGCCCTTCAACATCGCCTCCTCGGTGCTGTTGATCA
TGGCCCAGCGGCTCGCCCGGCGGCTCTGCGTCCATTGTCGGAAGAAACTCGATCTACCGCGCGAGGCGCTGCGCGAAGAG
GGGTTCTCCAATGAAGAAATCGACACCGGACTGACCATTTACGGCCCGGTCGGTTGCGAGAAATGCAACAAAGGTTATAA
AGGACGGGTTGGGATCTTTCAGGTCATGAAGGTGACGGATGCCATCACCCGCTTGATCCTACAGGGCGGCGACGCCATGC
AACTGGCCGATCAGGCACAACGCGAGGGGGTTTCGGATCTGCGCCAGTCCGGTCTGCGCAAGGTCAAGGCCGGACTGATC
GGCCTGGAAGAGCTCAATCGGGTCACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I3Y5P3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

55.319

98.774

0.546
  pilB Acinetobacter baylyi ADP1

53.927

100

0.541
  pilB Acinetobacter baumannii D1279779

53.472

100

0.539
  pilB Vibrio cholerae strain A1552

48.837

97.898

0.478
  pilB Vibrio parahaemolyticus RIMD 2210633

47.611

98.949

0.471
  pilB Vibrio campbellii strain DS40M4

46.975

98.424

0.462
  pilF Neisseria gonorrhoeae MS11

46.359

98.599

0.457
  pilF Thermus thermophilus HB27

40.214

98.424

0.396
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.256

91.594

0.387
  pilB/pilB1 Synechocystis sp. PCC 6803

40.734

90.718

0.37

Multiple sequence alignment