Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   I6H54_RS00305 Genome accession   NZ_CP066006
Coordinates   59045..60088 (-) Length   347 a.a.
NCBI ID   WP_012202999.1    Uniprot ID   A0A210VX04
Organism   Delftia acidovorans strain FDAARGOS_997     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 54045..65088
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6H54_RS00280 (I6H54_00280) - 54526..55134 (+) 609 WP_012202994.1 SIS domain-containing protein -
  I6H54_RS00285 (I6H54_00285) - 55131..55862 (+) 732 WP_034389978.1 BON domain-containing protein -
  I6H54_RS00290 (I6H54_00290) - 56086..56997 (-) 912 WP_034389980.1 NAD(P)-dependent oxidoreductase -
  I6H54_RS00295 (I6H54_00295) pilU 57123..58259 (-) 1137 WP_012202997.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  I6H54_RS00300 (I6H54_00300) - 58381..59013 (-) 633 WP_012202998.1 cyclic nucleotide-binding domain-containing protein -
  I6H54_RS00305 (I6H54_00305) pilT 59045..60088 (-) 1044 WP_012202999.1 type IV pilus twitching motility protein PilT Machinery gene
  I6H54_RS00310 (I6H54_00310) - 60126..60851 (+) 726 WP_034389983.1 YggS family pyridoxal phosphate-dependent enzyme -
  I6H54_RS00315 (I6H54_00315) - 60890..62173 (-) 1284 WP_034389985.1 branched-chain amino acid ABC transporter substrate-binding protein -
  I6H54_RS00320 (I6H54_00320) - 62200..62922 (-) 723 WP_034389987.1 TetR/AcrR family transcriptional regulator -
  I6H54_RS00325 (I6H54_00325) - 63082..64677 (+) 1596 WP_034389988.1 AMP-binding protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38309.02 Da        Isoelectric Point: 6.8352

>NTDB_id=516264 I6H54_RS00305 WP_012202999.1 59045..60088(-) (pilT) [Delftia acidovorans strain FDAARGOS_997]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDPLDHKTVHAMVYDIMSDSQRKAYEEFLEVDFSFEIEGLAR
FRVNAFNQYRGAAAVFRTIPSKILTLEQLNAPRIFGDLALKPRGLVLVTGPTGSGKSTTLAGMVNHLNETEYGHILTVED
PIEFVHDSKKCLINQREVGPMTQSFSAALRSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKTKDGQGRIAAHEIMLGTSAIRNLIREAKVAQMYSTIQTSQNVGMQTLDQN
LTDLVRRNLISPAEARAKAKIPDNFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=516264 I6H54_RS00305 WP_012202999.1 59045..60088(-) (pilT) [Delftia acidovorans strain FDAARGOS_997]
GTGGACATCACCCAATTGCTGGCATTCAGCGTCAAGAACAAGGCCTCCGACCTGCACCTGTCCGCGGGGCTGCCGCCGAT
GATCCGTGTGCACGGCGATGTGCGCCGCATCAATGTCGATCCGCTGGACCACAAGACCGTCCACGCCATGGTCTACGACA
TCATGAGCGACTCCCAGCGCAAGGCCTACGAGGAGTTCCTGGAGGTGGACTTCTCGTTCGAGATCGAGGGCCTGGCACGC
TTCCGTGTCAACGCCTTCAACCAGTACCGGGGCGCGGCCGCCGTCTTCCGGACCATTCCCAGCAAGATCCTGACGCTGGA
GCAGCTCAACGCGCCGCGCATCTTCGGCGACCTGGCCCTGAAGCCGCGCGGCCTGGTGCTGGTGACAGGCCCCACGGGCT
CGGGCAAGTCCACCACGCTGGCCGGCATGGTCAACCACCTCAACGAGACCGAGTACGGGCACATCCTGACGGTGGAGGAC
CCCATCGAATTCGTGCACGACTCCAAGAAGTGCCTGATCAACCAGCGTGAAGTCGGGCCGATGACGCAGTCGTTCTCGGC
CGCCCTGCGCTCGGCGCTGCGCGAGGACCCGGACGCCATCCTGGTGGGCGAAATGCGTGACCTGGAAACCATCCGCCTGG
CCATGACGGCGGCCGAGACGGGCCACCTGGTCTTCGGCACCCTGCACACCTCCAGCGCCGCCAAGACCATCGACCGCATC
ATCGACGTCTTCCCCGCCGAGGAAAAGGAGATGGTGCGCGCCATGCTCTCCGAATCGCTGCAGGCCGTGATCTCGCAGAC
GCTGTGCAAGACCAAGGACGGCCAGGGCCGCATCGCGGCCCACGAAATCATGCTGGGCACCAGCGCCATCCGCAACCTGA
TCCGCGAGGCCAAGGTCGCCCAGATGTACTCCACCATCCAGACGAGTCAGAATGTGGGCATGCAGACGCTGGACCAGAAC
CTGACCGATCTGGTGCGGCGCAACCTGATCAGCCCGGCGGAAGCCCGCGCCAAGGCCAAGATACCGGACAACTTCCCGGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A210VX04

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

75.652

99.424

0.752
  pilT Acinetobacter nosocomialis M2

75.362

99.424

0.749
  pilT Acinetobacter baumannii D1279779

75.362

99.424

0.749
  pilT Acinetobacter baumannii strain A118

75.362

99.424

0.749
  pilT Pseudomonas stutzeri DSM 10701

74.493

99.424

0.741
  pilT Acinetobacter baylyi ADP1

73.043

99.424

0.726
  pilT Legionella pneumophila strain Lp02

73.156

97.695

0.715
  pilT Legionella pneumophila strain ERS1305867

73.156

97.695

0.715
  pilT Neisseria meningitidis 8013

68.235

97.983

0.669
  pilT Neisseria gonorrhoeae MS11

67.941

97.983

0.666
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.276

98.271

0.651
  pilT Vibrio cholerae strain A1552

66.276

98.271

0.651
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

99.424

0.507
  pilU Vibrio cholerae strain A1552

42.985

96.542

0.415
  pilU Acinetobacter baylyi ADP1

41.329

99.712

0.412
  pilU Pseudomonas stutzeri DSM 10701

41.194

96.542

0.398