Detailed information NTG0515985

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: I6I21_RS09300
• Genome accession: NZ_CP065984
• Coordinates: 1811159..1811806 (+)
• Length: 215 a.a.
• NCBI ID: WP_004255240.1
• Uniprot ID: A0A1V0NHR8
• Organism: Lactococcus lactis strain FDAARGOS_1064
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1806159..1816806

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6I21_RS09275 (I6I21_09275)	-	1806288..1807466 (+)	1179	WP_014570696.1	SLC13 family permease	-
I6I21_RS09280 (I6I21_09280)	-	1807639..1808202 (+)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
I6I21_RS09285 (I6I21_09285)	-	1808306..1808704 (+)	399	WP_004255236.1	hypothetical protein	-
I6I21_RS09290 (I6I21_09290)	-	1808809..1809741 (+)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
I6I21_RS09295 (I6I21_09295)	-	1809738..1811099 (+)	1362	WP_004255239.1	ABC transporter permease	-
I6I21_RS09300 (I6I21_09300)	comEA	1811159..1811806 (+)	648	WP_004255240.1	ComEA family DNA-binding protein	Machinery gene
I6I21_RS09305 (I6I21_09305)	comEC	1811787..1813997 (+)	2211	WP_014570695.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
I6I21_RS09310 (I6I21_09310)	-	1814282..1815058 (+)	777	WP_004255244.1	alpha/beta hydrolase	-
I6I21_RS09315 (I6I21_09315)	-	1815242..1815457 (+)	216	WP_014570694.1	F0F1 ATP synthase subunit C	-
I6I21_RS09320 (I6I21_09320)	atpB	1815502..1816215 (+)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
I6I21_RS09325 (I6I21_09325)	atpF	1816230..1816736 (+)	507	WP_004255258.1	F0F1 ATP synthase subunit B	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23651.83 Da
• Isoelectric Point: 5.0062

>NTDB_id=515985 I6I21_RS09300 WP_004255240.1 1811159..1811806(+) (comEA) [Lactococcus lactis strain FDAARGOS_1064]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSKTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=515985 I6I21_RS09300 WP_004255240.1 1811159..1811806(+) (comEA) [Lactococcus lactis strain FDAARGOS_1064]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATTTAATTAAAGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAAAAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (151-212)

3D structure

• AlphaFold DB: A0A1V0NHR8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	75.688	100	0.767
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	36.564	100	0.386
comEA	Staphylococcus aureus MW2	37.089	99.07	0.367