Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   KYX59_RS20495 Genome accession   NZ_CP079780
Coordinates   3787858..3788298 (-) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain BL12     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3782858..3793298
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KYX59_RS20470 (KYX59_20365) coaE 3783348..3783968 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  KYX59_RS20475 (KYX59_20370) - 3783993..3784037 (+) 45 WP_120795372.1 protein YacM -
  KYX59_RS20480 (KYX59_20375) guaC 3784193..3785236 (+) 1044 WP_001217338.1 GMP reductase -
  KYX59_RS20485 (KYX59_20380) hofC 3785271..3786473 (-) 1203 WP_000157266.1 protein transport protein HofC -
  KYX59_RS20490 (KYX59_20385) pilB 3786463..3787848 (-) 1386 WP_122224766.1 type II secretion system protein GspE Machinery gene
  KYX59_RS20495 (KYX59_20390) pilA 3787858..3788298 (-) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  KYX59_RS20500 (KYX59_20395) nadC 3788501..3789394 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  KYX59_RS20505 (KYX59_20400) ampD 3789482..3790033 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  KYX59_RS20510 (KYX59_20405) ampE 3790030..3790884 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  KYX59_RS20515 (KYX59_20410) aroP 3790927..3792300 (-) 1374 WP_000969915.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=515499 KYX59_RS20495 WP_000360904.1 3787858..3788298(-) (pilA) [Escherichia coli strain BL12]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=515499 KYX59_RS20495 WP_000360904.1 3787858..3788298(-) (pilA) [Escherichia coli strain BL12]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363