Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   JC965_RS14610 Genome accession   NZ_CP065937
Coordinates   3031138..3032577 (+) Length   479 a.a.
NCBI ID   WP_069785531.1    Uniprot ID   A0A7T3WZN3
Organism   Aeromonas caviae strain Aero21     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3026138..3037577
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JC965_RS14580 (JC965_14580) mlaF 3026716..3027516 (-) 801 WP_069785529.1 phospholipid ABC transporter ATP-binding protein MlaF -
  JC965_RS14585 (JC965_14585) - 3027723..3028718 (+) 996 WP_029314845.1 KpsF/GutQ family sugar-phosphate isomerase -
  JC965_RS14590 (JC965_14590) kdsC 3028718..3029272 (+) 555 WP_041211851.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  JC965_RS14595 (JC965_14595) lptC 3029269..3029829 (+) 561 WP_069785530.1 LPS export ABC transporter periplasmic protein LptC -
  JC965_RS14600 (JC965_14600) lptA 3029816..3030334 (+) 519 WP_010675873.1 lipopolysaccharide transport periplasmic protein LptA -
  JC965_RS14605 (JC965_14605) lptB 3030347..3031072 (+) 726 WP_010675872.1 LPS export ABC transporter ATP-binding protein -
  JC965_RS14610 (JC965_14610) rpoN/rpoN1 3031138..3032577 (+) 1440 WP_069785531.1 RNA polymerase factor sigma-54 Machinery gene
  JC965_RS14615 (JC965_14615) hpf 3032598..3032885 (+) 288 WP_005305957.1 ribosome hibernation promoting factor -
  JC965_RS14620 (JC965_14620) ptsN 3032888..3033334 (+) 447 WP_010675870.1 PTS IIA-like nitrogen regulatory protein PtsN -
  JC965_RS14625 (JC965_14625) rapZ 3033375..3034241 (+) 867 WP_010675869.1 RNase adapter RapZ -
  JC965_RS14630 (JC965_14630) npr 3034262..3034534 (+) 273 WP_010675868.1 PTS phosphocarrier protein NPr -
  JC965_RS14635 (JC965_14635) - 3034550..3036453 (-) 1904 Protein_2853 HTH domain-containing protein -
  JC965_RS14640 (JC965_14640) - 3036452..3036760 (+) 309 WP_198497274.1 zinc ribbon domain-containing protein -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53687.53 Da        Isoelectric Point: 4.3928

>NTDB_id=515465 JC965_RS14610 WP_069785531.1 3031138..3032577(+) (rpoN/rpoN1) [Aeromonas caviae strain Aero21]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENEAQETSQAETQDASQLDSSDAMAQESMP
DELPVDTTWDEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIGESGYLTATLED
IQAAVSSEEVEVELDEIEAVLKRVQHFDPIGIAARSVQECLLIQLGQYSPDLPWLDEAREVIREHMDLLGNRDYRTLARK
TRLKESDLKEVLDLIQQLEPRPGNGIIQSESQYVIPDVSVVKKGSKWVVELNPDSAPRIRVNEAYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=515465 JC965_RS14610 WP_069785531.1 3031138..3032577(+) (rpoN/rpoN1) [Aeromonas caviae strain Aero21]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCACAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACCCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGACAACAACCCCTTGCTGGAGCAAGAGGAGAACGAGG
CGCAGGAAACCAGTCAGGCAGAGACACAGGACGCCAGTCAACTCGACTCCAGCGATGCCATGGCCCAGGAGAGCATGCCG
GATGAACTGCCGGTGGACACCACCTGGGACGAGGTCTACTCGGCCGGTACGGCCCAGAGTGCAGGCCCCATCCATGACGG
GGAAGACAGCGTCTATCAGGGGGAGACCACCGAGAACCTGCAGGACTACCTGCTGTGGCAGATGCGGCTCACCCCGTTCA
GTGATGTGGATGCTGCCATCGCCCTTGCCATCATCGATGCCATCGGCGAATCCGGGTATCTGACTGCCACCCTCGAGGAT
ATTCAGGCTGCAGTCAGCAGCGAGGAGGTAGAAGTCGAACTGGATGAGATCGAAGCCGTGCTCAAGCGGGTGCAGCATTT
CGATCCGATCGGCATCGCCGCCCGTTCGGTGCAAGAGTGTCTGCTGATCCAGCTCGGCCAGTATTCGCCGGACCTGCCCT
GGCTCGACGAGGCCAGAGAGGTGATCCGCGAACACATGGACCTGCTCGGCAATCGCGACTACCGCACTCTGGCTCGCAAG
ACCCGCCTCAAGGAGAGCGATCTCAAGGAGGTACTGGATCTTATCCAGCAACTGGAGCCCAGACCCGGCAACGGCATCAT
CCAGAGTGAATCCCAGTACGTGATCCCGGATGTGTCCGTGGTCAAGAAGGGCAGCAAGTGGGTGGTTGAACTCAATCCGG
ACTCTGCTCCGCGCATCCGTGTCAACGAGGCCTATGCGGCCATGGCGCGCAATGCTCGCAGCAGCACGGATACCCAGTTC
ATCCGTTCTCATTTGCAGGAAGCCAAGTGGTTCATCAAGAGCCTGGAGAGTCGTAACGACACCCTGCTCAAGGTGGCCAG
CTGCATCGTCGAGCAGCAGCAGGGCTTTTTCGAGTACGGCGAAGAGGCCATGAAACCCATGGTACTCAACGACATCGCGG
AAGCGGTGGAGATGCATGAGTCGACCATTTCACGGGTCACGACCCAGAAGTACATGCATACGCCCCGCGGCATTTTTGAA
CTGAAGTTTTTTTTCTCCAGTCACGTCAATACGGAGGGAGGAGGAGAGTGCTCATCGACCGCTATTCGCGCCCTGATCAA
GAAGCTGGTGACGGCGGAAAACCCCGCCAAGCCGCTTAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7T3WZN3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.276

100

0.48