Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KYX57_RS19275 Genome accession   NZ_CP079772
Coordinates   3961077..3961754 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain GS04     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3956077..3966754
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KYX57_RS19260 (KYX57_19190) chiA 3957444..3960137 (+) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -
  KYX57_RS19265 (KYX57_19195) bfd 3960306..3960500 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  KYX57_RS19270 (KYX57_19200) bfr 3960572..3961048 (+) 477 WP_000675504.1 bacterioferritin -
  KYX57_RS19275 (KYX57_19205) pilD 3961077..3961754 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  KYX57_RS19280 (KYX57_19210) gspM 3961754..3962215 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  KYX57_RS19285 (KYX57_19215) gspL 3962212..3963375 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  KYX57_RS19290 (KYX57_19220) gspK 3963390..3964373 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  KYX57_RS19295 (KYX57_19225) gspJ 3964366..3964953 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  KYX57_RS19300 (KYX57_19230) gspI 3964946..3965323 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  KYX57_RS19305 (KYX57_19235) gspH 3965320..3965829 (-) 510 WP_024238847.1 type II secretion system minor pseudopilin GspH -
  KYX57_RS19310 (KYX57_19240) gspG 3965837..3966274 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=515417 KYX57_RS19275 WP_000178161.1 3961077..3961754(-) (pilD) [Escherichia coli strain GS04]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=515417 KYX57_RS19275 WP_000178161.1 3961077..3961754(-) (pilD) [Escherichia coli strain GS04]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378