Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KX927_RS21105 Genome accession   NZ_CP079767
Coordinates   3860791..3861468 (-) Length   225 a.a.
NCBI ID   WP_000178154.1    Uniprot ID   P25960
Organism   Escherichia coli strain SF10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3855791..3866468
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KX927_RS21090 (KX927_20950) chiA 3857158..3859851 (+) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -
  KX927_RS21095 (KX927_20955) bfd 3860020..3860214 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  KX927_RS21100 (KX927_20960) bfr 3860286..3860762 (+) 477 WP_000675504.1 bacterioferritin -
  KX927_RS21105 (KX927_20965) pilD 3860791..3861468 (-) 678 WP_000178154.1 prepilin peptidase GspO Machinery gene
  KX927_RS21110 (KX927_20970) gspM 3861468..3861929 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  KX927_RS21115 (KX927_20975) gspL 3861926..3863089 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  KX927_RS21120 (KX927_20980) gspK 3863104..3864087 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  KX927_RS21125 (KX927_20985) gspJ 3864080..3864667 (-) 588 WP_259553561.1 type II secretion system minor pseudopilin GspJ -
  KX927_RS21130 (KX927_20990) gspI 3864660..3865037 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  KX927_RS21135 (KX927_20995) gspH 3865034..3865543 (-) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  KX927_RS21140 (KX927_21000) gspG 3865551..3865988 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24956.82 Da        Isoelectric Point: 7.6353

>NTDB_id=515253 KX927_RS21105 WP_000178154.1 3860791..3861468(-) (pilD) [Escherichia coli strain SF10]
MTMLLPLFILVGFIADYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=515253 KX927_RS21105 WP_000178154.1 3860791..3861468(-) (pilD) [Escherichia coli strain SF10]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P25960

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378