Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   JC286_RS06330 Genome accession   NZ_CP065921
Coordinates   1332074..1333048 (+) Length   324 a.a.
NCBI ID   WP_014613820.1    Uniprot ID   -
Organism   Staphylococcus pseudintermedius strain SP_11304-3A     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1327074..1338048
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JC286_RS06295 (JC286_06295) rpmG 1327458..1327622 (+) 165 WP_256726578.1 50S ribosomal protein L33 -
  JC286_RS06300 (JC286_06300) - 1327774..1328313 (+) 540 WP_014613814.1 5-formyltetrahydrofolate cyclo-ligase -
  JC286_RS06305 (JC286_06305) - 1328332..1329771 (+) 1440 WP_198460957.1 rhomboid family intramembrane serine protease -
  JC286_RS06310 (JC286_06310) - 1329773..1329976 (+) 204 WP_014613816.1 YqgQ family protein -
  JC286_RS06315 (JC286_06315) - 1329973..1330959 (+) 987 WP_198460966.1 ROK family glucokinase -
  JC286_RS06320 (JC286_06320) - 1330960..1331271 (+) 312 WP_014613818.1 MTH1187 family thiamine-binding protein -
  JC286_RS06325 (JC286_06325) - 1331284..1331913 (+) 630 WP_037542865.1 MBL fold metallo-hydrolase -
  JC286_RS06330 (JC286_06330) comGA 1332074..1333048 (+) 975 WP_014613820.1 competence type IV pilus ATPase ComGA Machinery gene
  JC286_RS06335 (JC286_06335) comGB 1333017..1334084 (+) 1068 WP_014613821.1 competence type IV pilus assembly protein ComGB -
  JC286_RS06340 (JC286_06340) comGC 1334097..1334419 (+) 323 Protein_1209 competence type IV pilus major pilin ComGC -
  JC286_RS06345 (JC286_06345) comGD 1334397..1334837 (+) 441 WP_014613823.1 competence type IV pilus minor pilin ComGD -
  JC286_RS06350 (JC286_06350) - 1334942..1335142 (+) 201 WP_242060551.1 hypothetical protein -
  JC286_RS06355 (JC286_06355) - 1335048..1335533 (+) 486 WP_080552896.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  JC286_RS06360 (JC286_06360) - 1335697..1336224 (+) 528 WP_014613826.1 shikimate kinase -
  JC286_RS06365 (JC286_06365) gcvT 1336382..1337473 (+) 1092 WP_065354204.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36846.58 Da        Isoelectric Point: 7.5584

>NTDB_id=515226 JC286_RS06330 WP_014613820.1 1332074..1333048(+) (comGA) [Staphylococcus pseudintermedius strain SP_11304-3A]
MQLLLDHVIQFALQNEASDIHFIPSQSQVEVKLRVKDQLVLYDTLNLETYQKLLTLLKYQAGLDISTRHKAQSGRYIYEH
KALYYLRVSTLPLNLGTESCVIRITPQYFQNAKAHSVLDIKSNMEKKQGLILFSGPTGAGKSTLMYQMVVYAKQKLNLNI
ITIEDPVEQLVNGITQISVNEKADITYGSSLKAILRCDPDVILIGEIRDSHIARDVIQASLSGHLVLSTIHANDCQGVLL
RLIEMGLSHQDLSQSINLIINQRLITTKNDQRQLVYETMDQQEIHYFLNNNFQLPTAFTSLSHKLQLLNKEGVIDEKILR
KYTI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=515226 JC286_RS06330 WP_014613820.1 1332074..1333048(+) (comGA) [Staphylococcus pseudintermedius strain SP_11304-3A]
ATGCAACTACTATTAGATCATGTAATACAATTTGCACTTCAAAATGAGGCATCAGACATTCATTTTATCCCATCACAATC
ACAAGTCGAAGTGAAACTCAGAGTGAAAGATCAACTTGTATTATATGACACATTAAATCTAGAAACGTATCAAAAATTGT
TAACTTTGTTGAAATACCAAGCTGGATTAGATATTTCGACACGCCACAAAGCGCAAAGTGGGCGCTATATTTATGAACAT
AAAGCGTTATATTATTTAAGAGTGTCCACGTTACCTTTAAACTTAGGTACTGAAAGTTGTGTTATTCGTATTACACCACA
ATATTTTCAAAATGCGAAAGCTCATAGTGTATTAGACATCAAATCTAATATGGAGAAAAAGCAAGGTTTAATTCTGTTTA
GCGGGCCTACAGGTGCAGGAAAGAGCACGTTAATGTATCAAATGGTCGTCTATGCTAAACAAAAGTTAAACCTGAATATT
ATCACAATAGAAGACCCGGTCGAGCAGCTTGTGAACGGCATTACGCAAATTTCCGTTAACGAAAAAGCTGATATTACGTA
TGGATCGTCGTTAAAAGCAATTCTTCGTTGTGATCCAGATGTCATCTTAATTGGTGAGATTCGTGACAGTCATATTGCAA
GGGACGTTATACAAGCGAGTTTAAGTGGGCATTTAGTATTGTCGACGATACACGCCAATGATTGTCAAGGGGTGCTCCTT
AGACTGATTGAAATGGGACTCTCTCACCAAGATTTATCTCAATCTATCAATCTCATCATCAATCAAAGACTCATTACAAC
AAAAAACGACCAGCGACAACTCGTCTATGAAACGATGGATCAACAGGAGATTCATTACTTTTTGAATAATAACTTCCAAC
TACCTACTGCTTTCACAAGCCTTTCTCATAAGCTTCAACTTCTGAATAAAGAGGGGGTTATAGATGAAAAGATTTTACGA
AAGTATACGATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

58.514

99.691

0.583
  comGA Staphylococcus aureus N315

58.514

99.691

0.583