Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   KX927_RS15245 Genome accession   NZ_CP079767
Coordinates   2624382..2624822 (+) Length   146 a.a.
NCBI ID   WP_029392818.1    Uniprot ID   -
Organism   Escherichia coli strain SF10     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2619382..2629822
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KX927_RS15225 (KX927_15125) aroP 2620380..2621753 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  KX927_RS15230 (KX927_15130) ampE 2621796..2622650 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  KX927_RS15235 (KX927_15135) ampD 2622647..2623198 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  KX927_RS15240 (KX927_15140) nadC 2623286..2624179 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  KX927_RS15245 (KX927_15145) pilA 2624382..2624822 (+) 441 WP_029392818.1 prepilin peptidase-dependent pilin Machinery gene
  KX927_RS15250 (KX927_15150) pilB 2624832..2626217 (+) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  KX927_RS15255 (KX927_15155) hofC 2626207..2627409 (+) 1203 WP_000157266.1 protein transport protein HofC -
  KX927_RS15260 (KX927_15160) guaC 2627444..2628487 (-) 1044 WP_001217338.1 GMP reductase -
  KX927_RS15265 (KX927_15165) - 2628643..2628687 (-) 45 WP_120795372.1 protein YacM -
  KX927_RS15270 (KX927_15170) coaE 2628712..2629332 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15607.73 Da        Isoelectric Point: 4.3938

>NTDB_id=515222 KX927_RS15245 WP_029392818.1 2624382..2624822(+) (pilA) [Escherichia coli strain SF10]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMSPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=515222 KX927_RS15245 WP_029392818.1 2624382..2624822(+) (pilA) [Escherichia coli strain SF10]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GTCACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363