Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   I7845_RS02110 Genome accession   NZ_CP065824
Coordinates   492295..493620 (+) Length   441 a.a.
NCBI ID   WP_218820636.1    Uniprot ID   -
Organism   Pseudomonas tolaasii strain Pt11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 487295..498620
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I7845_RS02090 (I7845_02090) - 487378..489840 (-) 2463 WP_371132213.1 penicillin-binding protein 1A -
  I7845_RS02095 (I7845_02095) pilM 490061..490717 (+) 657 WP_218820635.1 type IV pilus biogenesis protein PilM -
  I7845_RS02100 (I7845_02100) - 490714..491265 (+) 552 WP_218819926.1 PilN domain-containing protein -
  I7845_RS02105 (I7845_02105) - 491262..492224 (+) 963 WP_218819927.1 pilus assembly protein PilP -
  I7845_RS02110 (I7845_02110) pilQ 492295..493620 (+) 1326 WP_218820636.1 type IV pilus secretin PilQ Machinery gene
  I7845_RS02115 (I7845_02115) aroK 493625..494143 (+) 519 WP_016972712.1 shikimate kinase AroK -
  I7845_RS02120 (I7845_02120) aroB 494266..495366 (+) 1101 WP_016972711.1 3-dehydroquinate synthase -
  I7845_RS02125 (I7845_02125) - 495377..496942 (+) 1566 WP_177006725.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 441 a.a.        Molecular weight: 47775.69 Da        Isoelectric Point: 4.7136

>NTDB_id=514770 I7845_RS02110 WP_218820636.1 492295..493620(+) (pilQ) [Pseudomonas tolaasii strain Pt11]
MAFAVPNRVDLIQLPPPGNAKAGASTGDTLTLNFQNVELRTVLQQIADVAGLNLVASDEVQGSITLRLKDVPWDQALDLV
LQTKGLDKRVKAGVLLVAPAEELAARELLALEANKQMAELAPLRRELLQVNYAKASDLAKLFQSVTGLEGVTDERGSVAV
DDRTNNIIAYQTGERLEELRRIVAQLDVPVRQVMIEARIVEANVDYDKSLGVRWGGRLSRGNWGAGGIGKPLAEGVEPPE
IPSGSPFVDLGSLTGTSGLGLAFITDNLLLDLELTAMEKSGNGEIVSQPKVVTSDKETARILKGTEIPYQESTSQGATSV
SFKEASLSLEVTPQITPDDRVIMDVRVTKDEPDYLNKLNDVPPIKKNEVNAKVLVKDGETIVIGGVFSNTQSKVVDKVPF
LGDVPYLGRLFRRDVLAERKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 1326 bp        

>NTDB_id=514770 I7845_RS02110 WP_218820636.1 492295..493620(+) (pilQ) [Pseudomonas tolaasii strain Pt11]
ATGGCGTTTGCTGTGCCCAATCGCGTCGACCTGATCCAGCTGCCGCCTCCTGGTAATGCCAAGGCGGGCGCGTCTACCGG
CGACACACTTACGCTCAACTTCCAGAACGTTGAGCTGCGCACAGTGCTGCAGCAGATTGCCGACGTGGCCGGCCTGAACC
TGGTGGCCAGCGACGAGGTGCAGGGTTCGATCACCCTGCGGCTCAAGGATGTGCCTTGGGATCAGGCGCTGGACCTGGTG
TTACAGACCAAGGGGCTGGATAAGCGTGTGAAGGCGGGGGTTTTATTGGTGGCGCCGGCTGAAGAGCTGGCGGCCCGCGA
GTTGCTGGCACTGGAGGCAAACAAGCAGATGGCCGAGCTTGCGCCCTTGCGTCGTGAGCTGTTACAGGTCAATTACGCCA
AAGCCTCTGACCTCGCCAAGTTGTTCCAGTCGGTGACGGGCCTTGAAGGCGTCACTGATGAACGCGGTTCGGTGGCGGTG
GACGACCGGACCAACAACATCATTGCCTACCAGACCGGTGAGCGGCTTGAGGAGCTGCGGCGGATCGTGGCTCAGCTGGA
CGTCCCGGTGCGCCAGGTGATGATCGAGGCGCGAATTGTGGAAGCGAACGTGGACTATGACAAAAGCCTGGGCGTGCGCT
GGGGCGGGCGCCTCAGCCGTGGCAACTGGGGCGCTGGAGGAATTGGCAAACCCTTGGCTGAGGGTGTCGAACCACCGGAA
ATTCCGTCCGGCTCGCCGTTTGTTGACCTTGGCTCACTCACCGGCACTTCCGGGCTGGGCCTCGCCTTTATCACCGACAA
CCTTTTGTTGGATCTGGAACTGACCGCCATGGAGAAGTCCGGCAACGGCGAAATCGTCTCACAGCCCAAAGTGGTCACGT
CCGACAAAGAAACCGCGCGCATTCTCAAGGGCACCGAAATTCCGTATCAGGAATCCACCTCCCAGGGCGCCACGTCGGTA
TCGTTCAAGGAAGCTTCGCTGTCATTGGAGGTTACGCCGCAAATTACGCCGGATGACCGGGTAATCATGGACGTGCGCGT
GACCAAGGACGAACCCGATTACCTGAACAAGCTCAACGATGTGCCGCCGATCAAGAAAAACGAGGTCAACGCCAAGGTAT
TGGTCAAGGATGGCGAGACCATCGTCATCGGCGGGGTTTTCTCCAACACCCAGAGCAAAGTGGTAGATAAAGTGCCATTT
TTGGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGCTGGCGGAGCGAAAATCCGAGCTGCTGGTGTTCCT
CACTCCGCGTATTATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(127-192)

Secretin and TonB N terminus short domain (STN)

(53-99)

Bacterial type II and III secretion system protein (Secretin)

(276-432)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.938

99.093

0.703
  pilQ Acinetobacter baumannii strain A118

40.365

100

0.451
  pilQ Acinetobacter baumannii D1279779

40.365

100

0.451
  comQ Acinetobacter baylyi ADP1

40.625

100

0.442
  pilQ Vibrio campbellii strain DS40M4

44.444

95.918

0.426
  pilQ Vibrio cholerae strain A1552

44.63

95.011

0.424
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

44.63

95.011

0.424
  pilQ Legionella pneumophila strain Lp02

43.868

96.145

0.422
  pilQ Legionella pneumophila strain ERS1305867

43.396

96.145

0.417
  pilQ Neisseria meningitidis M1080

35.371

100

0.367
  pilQ Neisseria meningitidis MC58

35.463

100

0.365