Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   I8T81_RS16225 Genome accession   NZ_CP065820
Coordinates   3516096..3516896 (-) Length   266 a.a.
NCBI ID   WP_004698762.1    Uniprot ID   -
Organism   Acinetobacter seifertii strain S21     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3511096..3521896
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I8T81_RS16205 (I8T81_16205) - 3512161..3512859 (-) 699 WP_002053293.1 tetratricopeptide repeat protein -
  I8T81_RS16210 (I8T81_16210) hisS 3512888..3514180 (-) 1293 WP_198071650.1 histidine--tRNA ligase -
  I8T81_RS16215 (I8T81_16215) ispG 3514177..3515292 (-) 1116 WP_004698760.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  I8T81_RS16220 (I8T81_16220) - 3515314..3516105 (-) 792 WP_198071651.1 RodZ domain-containing protein -
  I8T81_RS16225 (I8T81_16225) pilF 3516096..3516896 (-) 801 WP_004698762.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  I8T81_RS16230 (I8T81_16230) rlmN 3516912..3518144 (-) 1233 WP_004698763.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  I8T81_RS16235 (I8T81_16235) ndk 3518267..3518698 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  I8T81_RS16240 (I8T81_16240) iscX 3518812..3519012 (-) 201 WP_004698764.1 Fe-S cluster assembly protein IscX -
  I8T81_RS16245 (I8T81_16245) - 3519112..3519663 (-) 552 WP_019458079.1 phosphatase PAP2 family protein -
  I8T81_RS16250 (I8T81_16250) - 3519688..3520986 (-) 1299 WP_198072785.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30125.50 Da        Isoelectric Point: 9.2307

>NTDB_id=514753 I8T81_RS16225 WP_004698762.1 3516096..3516896(-) (pilF) [Acinetobacter seifertii strain S21]
MSTPKLKIMLCMGVAVAFLASGCQTSPTLKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSIESRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAVEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=514753 I8T81_RS16225 WP_004698762.1 3516096..3516896(-) (pilF) [Acinetobacter seifertii strain S21]
TTGAGTACTCCGAAGTTAAAGATCATGTTATGTATGGGGGTTGCAGTTGCATTTCTGGCAAGTGGTTGTCAGACGTCGCC
AACACTAAAAAAGGACCCGGAAAAAGCAGTTAAGGTGCGTACGCAGCTTGCTGCAGAATACATTCGTTCTGGTGACTTGG
ACTCAGCAAAACGTTCCCTCGACCAAGCTCTAAGTATCGAGAGTCGTGACGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCAGAACATTATTTTAAACGTGCAATTTCTTCTGAGCCAGATAA
TGCTCAGGCGCGTAATAATTATGGTACGTATTTGTATCAAATGGAACGTTATAATGATGCTGTCGAGCAGTTCCGTATCG
CTGGTGCAACATTAGGTTATGACCAACGTTACCAAGCCTTAGAAAATCTGGGGCGGATTTATTTAAAATTAGGTGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCGCTCCTTGCTAACCGTGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCTCGTGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAATCAA
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

97.744

100

0.977
  pilF Acinetobacter baylyi ADP1

74.231

97.744

0.726