Detailed information NTG0514650

Bioinformatically predicted

Overview

• Name: radA
• Type: Machinery gene
• Locus tag: I7831_RS00830
• Genome accession: NZ_CP065797
• Coordinates: 159629..160999 (-)
• Length: 456 a.a.
• NCBI ID: WP_037539109.1
• Uniprot ID: A0A380HQT6
• Organism: Staphylococcus saprophyticus strain GDY8P168P
• Function: homologous recombination (predicted from homology) Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IS/Tn	161170..162342	159629..160999	flank	171

Gene organization within MGE regions

Location: 159629..162342

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I7831_RS00830 (I7831_00830)	radA	159629..160999 (-)	1371	WP_037539109.1	DNA repair protein RadA	Machinery gene
I7831_RS00835 (I7831_00835)	-	161170..162342 (+)	1173	WP_000195429.1	IS256-like element IS256 family transposase	-

Sequence

Protein

• Length: 456 a.a.
• Molecular weight: 50336.91 Da
• Isoelectric Point: 7.4848

>NTDB_id=514650 I7831_RS00830 WP_037539109.1 159629..160999(-) (radA) [Staphylococcus saprophyticus strain GDY8P168P]
MAKKKVIFECMACGYQSPKWMGKCPNCGGWNQMEEIVEQKAASPKHGVRSRDNVSKVQKLNEIKHETTPRVKTNSNEFNR
VLGGGIVNGSLVLIGGDPGIGKSTLLLQICASLSQSKNVLYITGEESLSQTKLRAERLDEDSSELNVFAETDLEVIHETV
KQIKPDLLVVDSIQTIFHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGD
EHHAYRILRAVKNRFGSTNEMGIFEMKQTGLKGVKNPSEMFLEERSANVPGSTIVSTMEGTRPLLIEVQALVTPTTFNNP
RRMATGIDHNRLSLLMAVLEKKENYLLQQQDAYIKVAGGVRLTEPAVDLGIIIATASSFKDLTVDGLDCFIGEVGLTGEV
RRVSRIEQRVQEAEKLGFKRVIIPESNIGGWQFPEGIKVIGVKSVHEALKYALTKH

Nucleotide

• Length: 1371 bp

>NTDB_id=514650 I7831_RS00830 WP_037539109.1 159629..160999(-) (radA) [Staphylococcus saprophyticus strain GDY8P168P]
TTGGCTAAAAAGAAAGTGATTTTTGAATGTATGGCATGTGGTTATCAGTCACCGAAATGGATGGGGAAATGCCCAAACTG
TGGCGGTTGGAATCAAATGGAAGAAATTGTCGAACAAAAGGCAGCCAGTCCAAAACATGGCGTACGCAGTCGAGACAATG
TATCTAAAGTACAAAAATTAAATGAAATTAAACATGAAACAACACCTAGGGTTAAAACAAATTCAAACGAATTTAACCGC
GTACTAGGTGGAGGTATTGTTAATGGTTCACTTGTCTTAATTGGTGGTGATCCTGGTATTGGGAAATCGACATTACTATT
ACAAATTTGTGCATCATTATCCCAATCTAAAAACGTATTATATATCACTGGTGAAGAATCATTGAGTCAAACGAAATTAA
GAGCGGAAAGATTAGATGAAGATTCTAGTGAATTAAATGTATTTGCCGAAACAGATTTAGAAGTAATCCATGAAACGGTT
AAACAAATTAAACCAGATTTACTTGTTGTGGATTCTATACAAACAATATTCCACCCTGAAATCAGCTCGGCACCAGGCTC
GGTGTCACAAGTTAGAGAAAGTACGCAGAGCTTAATGAATATAGCTAAACAGATGAATATTGCGACGTTTATTGTCGGAC
ATGTAACAAAAGAAGGTCAAATCGCTGGGCCGCGTTTACTTGAACATATGGTGGACACAGTGCTTTACTTCGAAGGTGAT
GAACACCACGCATATCGTATTTTAAGAGCTGTGAAAAACCGTTTTGGTTCTACAAATGAAATGGGTATCTTTGAGATGAA
GCAAACAGGTTTGAAAGGCGTTAAAAACCCTTCGGAAATGTTTCTTGAAGAACGTTCAGCCAATGTACCAGGTTCCACGA
TTGTTTCTACCATGGAAGGTACGAGACCTTTATTGATTGAAGTACAAGCATTAGTGACACCGACAACTTTTAATAATCCG
AGACGTATGGCAACGGGCATAGATCATAATCGATTGAGTTTGCTCATGGCCGTATTAGAAAAGAAAGAAAATTATCTCCT
TCAGCAGCAAGATGCTTATATCAAAGTTGCGGGTGGTGTGAGACTTACTGAGCCAGCAGTAGATTTAGGTATCATTATTG
CGACGGCTTCAAGCTTTAAAGATTTAACGGTGGACGGCTTGGACTGTTTCATCGGTGAAGTAGGTCTTACTGGAGAAGTC
AGACGTGTTTCTCGAATAGAACAACGTGTCCAAGAAGCTGAAAAATTAGGGTTCAAACGTGTGATTATCCCTGAGTCCAA
TATCGGGGGATGGCAATTTCCTGAAGGTATAAAAGTAATTGGGGTTAAAAGTGTTCATGAAGCTTTAAAATACGCATTAA
CAAAACATTAA

Domains

Predicted by InterproScan.

• Subunit ChlI of Mg-chelatase (ChlI) (360-427)

• Rubredoxin metal binding domain (Zn_ribbon_LapB) (8-35)

• AAA domain (AAA_25) (67-219)

3D structure

• AlphaFold DB: A0A380HQT6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
radA	Bacillus subtilis subsp. subtilis str. 168	65.789	100	0.658
radA	Streptococcus pneumoniae Rx1	60	98.684	0.592
radA	Streptococcus pneumoniae D39	60	98.684	0.592
radA	Streptococcus pneumoniae R6	60	98.684	0.592
radA	Streptococcus pneumoniae TIGR4	60	98.684	0.592
radA	Streptococcus mitis NCTC 12261	60	98.684	0.592
radA	Streptococcus mitis SK321	60	98.684	0.592