Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   I7827_RS01810 Genome accession   NZ_CP065792
Coordinates   354789..356159 (-) Length   456 a.a.
NCBI ID   WP_025904845.1    Uniprot ID   A0A2T4RU06
Organism   Mammaliicoccus sciuri strain GDK8D6P     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 349789..361159
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I7827_RS01785 (I7827_01785) cysE 350182..350823 (-) 642 WP_025904850.1 serine O-acetyltransferase -
  I7827_RS01790 (I7827_01790) gltX 351078..352532 (-) 1455 WP_048541341.1 glutamate--tRNA ligase -
  I7827_RS01795 (I7827_01795) ispF 352548..353027 (-) 480 WP_198054404.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  I7827_RS01800 (I7827_01800) ispD 353020..353706 (-) 687 WP_198054405.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  I7827_RS01805 (I7827_01805) - 353708..354766 (-) 1059 WP_037589912.1 PIN/TRAM domain-containing protein -
  I7827_RS01810 (I7827_01810) radA 354789..356159 (-) 1371 WP_025904845.1 DNA repair protein RadA Machinery gene
  I7827_RS01815 (I7827_01815) - 356315..358777 (-) 2463 WP_025904844.1 ATP-dependent Clp protease ATP-binding subunit -
  I7827_RS01820 (I7827_01820) - 358794..359801 (-) 1008 WP_025904843.1 protein arginine kinase -
  I7827_RS01825 (I7827_01825) - 359802..360338 (-) 537 WP_025904842.1 UvrB/UvrC motif-containing protein -
  I7827_RS01830 (I7827_01830) - 360353..360814 (-) 462 WP_025904841.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 50477.24 Da        Isoelectric Point: 7.7103

>NTDB_id=514586 I7827_RS01810 WP_025904845.1 354789..356159(-) (radA) [Mammaliicoccus sciuri strain GDK8D6P]
MAKKKVIFECLACGYQSPKWMGKCPNCGAWNQMEESIEHKETKGPKNTFKSDVPAQKIEKLKDVKEEDTPRIKTKINEFN
RVLGGGVVEGSLVLIGGDPGIGKSTLLLQICAALSQQKNVLYVSGEESVRQTKLRAERLVEDSGELNVYAETNLEIIHSM
VQKEKPDVLVIDSIQTVFHPEVTSAPGSVSQVRECTQNLMHIAKQMNIATFIVGHVTKDGQIAGPRLLEHMVDTVLYFEG
DTHHAYRILRAVKNRFGSTNEMGIFEMKQTGLNEVKNPSEMFLEERTKHVSGSTIVATMEGTRPILVEVQALVTPTTFHN
PRRMATGIDHNRLSLLMAVLEKKENFLLQQQDAYIKIAGGVKLSEPAVDLSIVIAIASSFQDKPTKGDDCFVGEVGLTGE
VRRVARIDQRVQEAAKLGFKRIIIPKNNIKGLEFPEGTEVIGVSTTREALKIAFNA

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=514586 I7827_RS01810 WP_025904845.1 354789..356159(-) (radA) [Mammaliicoccus sciuri strain GDK8D6P]
ATGGCCAAAAAGAAAGTAATATTTGAGTGTTTAGCCTGCGGATATCAATCTCCAAAATGGATGGGGAAATGCCCTAATTG
TGGTGCATGGAACCAAATGGAAGAATCAATAGAACATAAAGAAACTAAAGGTCCTAAAAATACATTCAAGTCAGATGTAC
CCGCACAAAAAATAGAAAAATTAAAAGACGTTAAAGAAGAAGATACACCTAGAATTAAGACAAAAATCAATGAATTTAAT
CGCGTATTAGGTGGCGGTGTTGTAGAAGGTTCACTTGTACTCATTGGTGGTGACCCTGGTATTGGTAAATCCACATTATT
ATTACAAATTTGTGCGGCTTTATCTCAACAAAAAAATGTCTTATATGTTTCAGGTGAAGAATCTGTAAGACAAACAAAAT
TAAGAGCTGAAAGATTAGTTGAAGATTCAGGAGAATTAAATGTTTATGCTGAAACGAATTTAGAGATTATACATAGTATG
GTTCAAAAAGAAAAACCAGATGTATTAGTAATAGATTCCATTCAAACTGTCTTCCACCCTGAAGTCACTTCTGCGCCAGG
TTCTGTTTCTCAAGTTAGAGAATGTACACAAAATTTAATGCATATCGCAAAACAAATGAACATTGCTACATTTATTGTTG
GACACGTAACGAAAGATGGACAAATTGCTGGTCCACGTTTGTTAGAACATATGGTAGATACAGTATTATATTTTGAAGGC
GATACACATCATGCATACAGAATATTAAGAGCAGTTAAAAACCGTTTTGGTTCAACAAATGAAATGGGTATTTTTGAAAT
GAAGCAAACGGGTCTTAACGAAGTTAAAAATCCGTCTGAAATGTTCTTAGAAGAAAGAACGAAACATGTATCGGGTTCGA
CCATTGTCGCAACTATGGAAGGTACGAGACCTATTTTAGTTGAAGTACAAGCTTTAGTGACGCCAACGACTTTTCATAAT
CCACGAAGAATGGCAACAGGTATTGATCACAATAGATTAAGCTTATTGATGGCTGTATTAGAGAAGAAAGAGAATTTCTT
ATTGCAACAACAAGACGCATATATCAAAATTGCCGGTGGTGTAAAATTAAGTGAACCCGCAGTAGATTTAAGTATTGTTA
TCGCGATAGCTTCAAGTTTCCAAGATAAACCTACTAAAGGTGATGATTGTTTCGTAGGTGAGGTTGGCTTAACAGGTGAA
GTAAGAAGGGTTGCTAGAATTGATCAACGTGTACAAGAAGCGGCTAAATTAGGATTTAAACGTATTATCATACCTAAAAA
TAATATTAAAGGTTTAGAATTCCCTGAAGGTACAGAAGTTATTGGCGTATCAACAACAAGAGAAGCATTGAAAATTGCTT
TTAATGCATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(67-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(349-428)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T4RU06

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

65.721

100

0.66
  radA Streptococcus pneumoniae Rx1

61.894

99.561

0.616
  radA Streptococcus pneumoniae D39

61.894

99.561

0.616
  radA Streptococcus pneumoniae R6

61.894

99.561

0.616
  radA Streptococcus pneumoniae TIGR4

61.894

99.561

0.616
  radA Streptococcus mitis NCTC 12261

61.894

99.561

0.616
  radA Streptococcus mitis SK321

61.894

99.561

0.616