Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   I8N73_RS06025 Genome accession   NZ_CP065791
Coordinates   1199133..1200962 (+) Length   609 a.a.
NCBI ID   WP_094031915.1    Uniprot ID   -
Organism   Bacillus velezensis strain LBUM279     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 1194133..1205962
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I8N73_RS05995 (I8N73_05995) - 1194796..1195374 (+) 579 WP_007409110.1 GNAT family N-acetyltransferase -
  I8N73_RS06000 (I8N73_06000) spx 1195553..1195948 (+) 396 WP_003155034.1 transcriptional regulator Spx -
  I8N73_RS06005 (I8N73_06005) - 1196006..1196662 (-) 657 WP_015239570.1 TerC family protein -
  I8N73_RS06015 (I8N73_06015) mecA 1196938..1197594 (+) 657 WP_003155032.1 adaptor protein MecA Regulator
  I8N73_RS06020 (I8N73_06020) - 1197745..1198905 (+) 1161 WP_069006905.1 competence protein CoiA family protein -
  I8N73_RS06025 (I8N73_06025) pepF 1199133..1200962 (+) 1830 WP_094031915.1 oligoendopeptidase F Regulator
  I8N73_RS06030 (I8N73_06030) - 1201000..1201167 (-) 168 WP_003155026.1 hypothetical protein -
  I8N73_RS06035 (I8N73_06035) spxH 1201453..1202355 (-) 903 WP_032874464.1 protease adaptor protein SpxH -
  I8N73_RS06040 (I8N73_06040) - 1202352..1202750 (-) 399 WP_003155023.1 thiol management oxidoreductase -
  I8N73_RS06045 (I8N73_06045) - 1202979..1203665 (-) 687 WP_007409105.1 lytic transglycosylase domain-containing protein -
  I8N73_RS06050 (I8N73_06050) - 1203670..1204242 (-) 573 WP_014417426.1 CYTH domain-containing protein -
  I8N73_RS06055 (I8N73_06055) - 1204367..1204732 (+) 366 WP_003155020.1 hypothetical protein -
  I8N73_RS06060 (I8N73_06060) yjbM 1204760..1205395 (+) 636 WP_007610625.1 GTP diphosphokinase -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 69827.48 Da        Isoelectric Point: 5.2042

>NTDB_id=514536 I8N73_RS06025 WP_094031915.1 1199133..1200962(+) (pepF) [Bacillus velezensis strain LBUM279]
MTEENKAAQLPDRSEVKAEHTWRLEDIFPSDEAWNKEFQAVKELIPNLSSFKGTLAHSADNLYAALTAQDEVTNRLGKLY
AYAHMRSDQDTGNSFYQGLNDKAASLYSQAASATAYIEPEILAIQEDKLHQFILEKEELKLYSHAIEEITKQRPHVLSEQ
EEALLAEASEALSSPSNTFSVLNNADITFPSIKDENGKETQITHGNFINFLESSNREVRKNAFEAVYKTYGQYKNTMATT
LSGTVKKDNFYARVKKYKSAREAALSNNSIPEEVYDNLIKTINKHLPLLHRYIDLRKKVLGLDEVHIYDLYTPLVKDSGM
KVTYEEAKEYMLKGLAPLGEEYASILKEGLENRWVDIYENKGKRSGAYSSGTYGTNPYILMNWHDNVNNLFTLVHEFGHS
VHSYYTRKYQPYPYGNYSIFVAEVASTTNEALLGEYMLNSLKDEKQRLYLLNHMLEGFRGTVFRQTMFAEFEHLIHTKAQ
EGEPLTPELLNELYYDLNKKYFGDNMVIDKEISLEWSRIPHFYYNYYVYQYATGYSAAQALSSQILKEGKPAVERYTEFL
KAGSSEYPIDILKKAGVDMTSPEPIEAACKMFEEKLNEMEELLSKVNPS

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=514536 I8N73_RS06025 WP_094031915.1 1199133..1200962(+) (pepF) [Bacillus velezensis strain LBUM279]
ATGACTGAGGAAAATAAAGCAGCACAGCTGCCTGACAGAAGTGAAGTAAAGGCAGAGCATACATGGAGACTAGAGGATAT
TTTTCCTAGTGATGAGGCCTGGAATAAAGAATTTCAAGCAGTTAAAGAATTAATCCCGAATTTATCTTCTTTTAAAGGCA
CGCTTGCTCATTCGGCTGATAATTTATACGCGGCGCTTACGGCACAGGATGAAGTGACGAACCGCCTTGGGAAACTTTAT
GCATACGCGCATATGCGCTCAGACCAGGATACGGGAAATTCTTTCTACCAAGGGCTGAACGACAAAGCCGCAAGCCTGTA
TTCCCAGGCTGCCAGCGCGACTGCTTACATTGAACCGGAAATTCTGGCGATTCAGGAGGACAAGCTGCACCAGTTTATTC
TGGAAAAAGAGGAGCTTAAACTTTACTCCCATGCAATTGAAGAAATTACAAAACAGCGTCCGCACGTGCTGAGCGAACAA
GAAGAGGCCCTGCTTGCGGAAGCCTCAGAAGCGCTGTCGTCGCCTTCCAATACATTCAGTGTTTTAAATAACGCCGATAT
TACGTTTCCGTCGATTAAAGATGAAAACGGCAAGGAAACTCAAATCACACACGGCAACTTTATTAATTTCCTGGAAAGCA
GCAATCGTGAAGTCCGCAAAAACGCATTTGAAGCCGTATATAAAACATACGGACAGTATAAAAACACGATGGCGACTACA
CTCAGCGGCACTGTGAAAAAAGATAATTTTTACGCCCGTGTGAAAAAGTATAAATCAGCACGGGAAGCGGCTTTATCCAA
TAACAGCATCCCTGAAGAAGTTTACGATAACCTTATTAAAACGATCAATAAACATCTTCCGTTATTACACCGTTACATTG
ACCTGCGGAAAAAGGTTCTCGGCCTTGATGAAGTCCATATTTATGACCTTTACACACCGCTCGTAAAAGATTCGGGAATG
AAGGTCACATATGAGGAAGCGAAAGAGTATATGCTCAAAGGACTTGCTCCGCTGGGCGAGGAATACGCGTCTATTTTAAA
AGAAGGACTTGAAAACCGCTGGGTCGATATATACGAAAATAAAGGAAAACGCAGCGGTGCTTATTCGTCCGGTACATACG
GCACGAATCCGTACATCTTGATGAACTGGCATGACAATGTCAATAATCTGTTTACGCTCGTTCACGAGTTCGGCCATTCC
GTGCACAGCTACTATACGAGAAAATATCAGCCGTATCCGTACGGTAACTACAGCATTTTTGTCGCTGAAGTCGCTTCAAC
GACCAATGAAGCGCTGCTCGGTGAATATATGCTGAACAGCTTAAAGGATGAAAAACAGCGTCTGTACCTGCTCAACCACA
TGCTTGAAGGATTCAGAGGCACAGTGTTCAGACAGACGATGTTCGCTGAATTTGAGCATCTGATTCATACGAAAGCGCAG
GAAGGCGAGCCGCTAACGCCTGAACTGCTGAACGAACTTTATTATGATCTGAATAAAAAGTATTTCGGTGACAACATGGT
GATTGATAAAGAAATCAGTCTGGAATGGTCAAGAATTCCGCATTTCTATTACAATTATTATGTGTATCAATATGCGACAG
GCTACAGTGCGGCCCAAGCGCTGAGCAGCCAGATCTTAAAAGAAGGCAAGCCTGCGGTGGAGCGCTATACCGAATTCTTA
AAAGCGGGAAGCTCGGAATATCCGATCGACATACTGAAAAAAGCGGGAGTCGATATGACATCGCCTGAACCGATCGAGGC
GGCCTGCAAAATGTTTGAAGAGAAACTGAACGAAATGGAAGAGCTTCTTTCAAAAGTGAACCCTTCGTAG

Domains

Predicted by InterproScan.

Peptidase family M3 (Peptidase_M3)

(211-590)

Oligopeptidase F (Peptidase_M3_N)

(120-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

49.832

97.537

0.486