Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   I8N72_RS17775 Genome accession   NZ_CP065790
Coordinates   3599556..3600701 (+) Length   381 a.a.
NCBI ID   WP_015387560.1    Uniprot ID   -
Organism   Bacillus velezensis strain LBUM1082     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 3594556..3605701
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I8N72_RS17755 (I8N72_17755) - 3595030..3595746 (-) 717 WP_014306017.1 Crp/Fnr family transcriptional regulator -
  I8N72_RS17760 (I8N72_17760) narK 3595844..3597034 (-) 1191 WP_014306018.1 nitrate transporter NarK -
  I8N72_RS17765 (I8N72_17765) argS 3597169..3598839 (-) 1671 WP_003151071.1 arginine--tRNA ligase -
  I8N72_RS17770 (I8N72_17770) - 3598836..3599264 (-) 429 WP_003151069.1 DUF1934 domain-containing protein -
  I8N72_RS17775 (I8N72_17775) rapF 3599556..3600701 (+) 1146 WP_015387560.1 tetratricopeptide repeat protein Regulator
  I8N72_RS17780 (I8N72_17780) - 3600685..3600804 (+) 120 WP_015387559.1 PhrC/PhrF family phosphatase-inhibitory pheromone -
  I8N72_RS17785 (I8N72_17785) - 3601003..3601206 (+) 204 WP_003151064.1 helix-turn-helix transcriptional regulator -
  I8N72_RS17790 (I8N72_17790) - 3601220..3601423 (+) 204 WP_003151062.1 hypothetical protein -
  I8N72_RS17795 (I8N72_17795) - 3601832..3602113 (+) 282 WP_014306021.1 hypothetical protein -
  I8N72_RS17800 (I8N72_17800) - 3602157..3602669 (+) 513 WP_015387558.1 stage II sporulation protein M -
  I8N72_RS17805 (I8N72_17805) - 3603260..3603478 (-) 219 WP_003151056.1 helix-turn-helix transcriptional regulator -
  I8N72_RS17810 (I8N72_17810) speB 3603648..3604520 (-) 873 WP_003151054.1 agmatinase -
  I8N72_RS17815 (I8N72_17815) speE 3604580..3605410 (-) 831 WP_003151052.1 spermidine synthase -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45541.70 Da        Isoelectric Point: 4.6084

>NTDB_id=514507 I8N72_RS17775 WP_015387560.1 3599556..3600701(+) (rapF) [Bacillus velezensis strain LBUM1082]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEEDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQARLTGRLDYYFNFFRGMYELECREYLSAIQFFKKAEYKLDFVKDCIEKAEFYFKMSESYYYMKQTYFS
MDYARQAYKIYHKQEAYNIRVLQCHSLFATNFLDLKQYDEAIQHFKKAYAMAEAEQQPQLMGRTLYNIGLCFNSQGNYKP
AIDYIKRAIAVFEDGNIITSLPQAYFLITQIHYKIGNMAIARQYHDKGVSYAEEAEDSLYIVEYEFLESLYVGEPDEEAI
MECFDFLRDKMMYADLEDFALDVAKYYHERENFEKAAAYFLKVEETRQQIQGGVKLYEIEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=514507 I8N72_RS17775 WP_015387560.1 3599556..3600701(+) (rapF) [Bacillus velezensis strain LBUM1082]
GTGGCGAGTGTTTTATCGTCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAGCTTGCAGGCCTGGAAGAAGATCAGGATCTTCATCTGTACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGGCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGAATGCCGTGAATATCTTTCAGCGATTCAGTTTTTTAAGAAAGCTGAATATAAGCTGGATTTTG
TCAAAGATTGTATCGAGAAAGCTGAATTTTATTTTAAGATGTCAGAGTCCTACTATTATATGAAGCAGACTTATTTTTCT
ATGGATTATGCCCGGCAGGCTTATAAAATTTATCATAAGCAGGAGGCATATAATATAAGGGTGCTTCAGTGCCATTCTTT
GTTCGCTACCAACTTTCTTGACTTAAAGCAATATGATGAAGCAATTCAGCATTTTAAAAAGGCTTATGCCATGGCAGAGG
CAGAACAGCAGCCTCAATTAATGGGCCGGACTCTATATAATATCGGACTTTGTTTTAACAGCCAAGGAAATTACAAACCA
GCCATTGATTACATAAAACGGGCAATAGCTGTTTTTGAAGATGGGAACATTATCACTTCTCTCCCGCAAGCCTATTTCTT
AATCACACAAATACATTATAAAATCGGAAATATGGCCATAGCCAGACAATATCATGACAAAGGGGTATCTTATGCAGAGG
AGGCAGAAGATTCCCTATATATAGTCGAATATGAATTTTTAGAATCTTTGTATGTCGGTGAGCCTGATGAAGAAGCAATC
ATGGAATGTTTTGACTTTCTCAGAGATAAAATGATGTATGCTGATCTTGAAGATTTTGCCTTAGATGTGGCAAAATATTA
TCATGAAAGAGAGAATTTCGAGAAGGCTGCTGCATATTTTTTAAAGGTGGAAGAAACAAGACAGCAGATTCAAGGAGGTG
TGAAGTTGTATGAAATTGAAGTATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_12)

(189-253)

response regulator aspartate phosphatase H, N terminal (RapH_N)

(5-65)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

78.74

100

0.787
  rapC Bacillus subtilis subsp. subtilis str. 168

57.105

99.738

0.57