Detailed information NTG0514430

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: I8N71_RS20165
• Genome accession: NZ_CP065789
• Coordinates: 3845634..3846779 (+)
• Length: 381 a.a.
• NCBI ID: WP_003227549.1
• Uniprot ID: -
• Organism: Bacillus subtilis strain LBUM979
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 3840634..3851779

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I8N71_RS20145 (I8N71_20145)	albF	3840650..3841930 (+)	1281	WP_003242974.1	pitrilysin family protein	-
I8N71_RS20150 (I8N71_20150)	albG	3841927..3842628 (+)	702	WP_003243391.1	antilisterial bacteriocin subtilosin biosynthesis protein AlbG	-
I8N71_RS20155 (I8N71_20155)	ywhL	3842634..3844010 (-)	1377	WP_003244415.1	YncE family protein	-
I8N71_RS20160 (I8N71_20160)	ywhK	3844049..3845404 (-)	1356	WP_003227552.1	YncE family protein	-
I8N71_RS22290	-	3845401..3845631 (-)	231	WP_009968328.1	hypothetical protein	-
I8N71_RS20165 (I8N71_20165)	rapF	3845634..3846779 (+)	1146	WP_003227549.1	response regulator aspartate phosphatase RapF	Regulator
I8N71_RS20170 (I8N71_20170)	phrF	3846763..3846882 (+)	120	WP_009968329.1	phosphatase RapF inhibitor PhrF	Regulator
I8N71_RS20175 (I8N71_20175)	ywhH	3846981..3847454 (+)	474	WP_003227547.1	YbaK/EbsC family protein	-
I8N71_RS20180 (I8N71_20180)	speB	3847486..3848358 (-)	873	WP_003227545.1	agmatinase	-
I8N71_RS20185 (I8N71_20185)	speE	3848419..3849249 (-)	831	WP_003227543.1	spermidine synthase	-
I8N71_RS20190 (I8N71_20190)	pbpG	3849451..3851526 (+)	2076	WP_003227540.1	transglycosylase domain-containing protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 45609.09 Da
• Isoelectric Point: 5.1503

>NTDB_id=514430 I8N71_RS20165 WP_003227549.1 3845634..3846779(+) (rapF) [Bacillus subtilis strain LBUM979]
MTGVISSSSIGEKINEWYMYIRRFSIPDAEYLRREIKQELDQMEEDQDLHLYYSLMEFRHNLMLEYLEPLEKMRIEEQPR
LSDLLLEIDKKQARLTGLLEYYFNFFRGMYELDQREYLSAIKFFKKAESKLIFVKDRIEKAEFFFKMSESYYYMKQTYFS
MDYARQAYEIYKEHEAYNIRLLQCHSLFATNFLDLKQYEDAISHFQKAYSMAEAEKQPQLMGRTLYNIGLCKNSQSQYED
AIPYFKRAIAVFEESNILPSLPQAYFLITQIHYKLGKIDKAHEYHSKGMAYSQKAGDVIYLSEFEFLKSLYLSGPDEEAI
QGFFDFLESKMLYADLEDFAIDVAKYYHERKNFQKASAYFLKVEQVRQLIQGGVSLYEIEV

Nucleotide

• Length: 1146 bp

>NTDB_id=514430 I8N71_RS20165 WP_003227549.1 3845634..3846779(+) (rapF) [Bacillus subtilis strain LBUM979]
GTGACAGGTGTCATATCTTCTTCTTCCATCGGAGAAAAGATTAACGAATGGTATATGTACATACGCCGATTCAGCATACC
CGATGCAGAATATTTGCGACGAGAAATCAAGCAAGAGCTGGATCAAATGGAAGAAGATCAAGACCTTCATTTGTACTATT
CACTGATGGAGTTTCGGCACAACCTAATGCTTGAGTACCTTGAACCGTTAGAAAAAATGAGGATTGAGGAACAGCCGAGA
CTGTCTGATCTGCTGCTTGAGATTGATAAAAAACAGGCTCGTTTAACTGGTCTGCTTGAGTACTATTTTAACTTCTTCAG
AGGCATGTACGAGCTGGACCAGCGGGAATATCTGTCGGCTATTAAATTTTTCAAAAAGGCCGAAAGCAAGCTGATATTCG
TTAAGGATCGGATAGAGAAAGCTGAGTTTTTCTTTAAGATGTCTGAATCTTATTACTATATGAAACAAACGTATTTTTCA
ATGGACTATGCACGGCAAGCATATGAAATATACAAAGAACATGAAGCTTATAATATAAGATTGCTGCAGTGTCATTCTTT
ATTTGCCACCAATTTTTTAGATTTAAAACAGTATGAGGATGCCATCTCACATTTTCAAAAAGCTTATTCTATGGCAGAAG
CTGAAAAGCAGCCCCAATTAATGGGGAGAACTTTGTACAATATCGGGCTTTGTAAAAACAGCCAAAGCCAATATGAGGAT
GCCATACCTTATTTCAAAAGAGCAATAGCTGTTTTTGAAGAATCAAATATTCTTCCTTCCTTACCTCAAGCGTATTTTTT
AATTACACAGATCCATTATAAATTAGGAAAAATAGATAAAGCTCATGAATATCATAGTAAGGGAATGGCTTATTCACAAA
AGGCCGGAGATGTAATATATTTATCAGAGTTTGAATTTTTGAAATCTTTATACTTATCAGGCCCGGATGAAGAAGCAATT
CAAGGATTTTTTGATTTTCTCGAAAGTAAAATGTTGTATGCTGATCTTGAAGATTTCGCTATTGATGTGGCAAAATATTA
TCATGAACGTAAAAATTTTCAAAAAGCTTCTGCTTATTTTTTGAAGGTGGAACAAGTAAGGCAACTTATTCAAGGAGGAG
TGAGTTTGTATGAAATTGAAGTCTAA

Domains

Predicted by InterproScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Tetratricopeptide repeat (TPR_12) (223-290)

• Anaphase-promoting complex, cyclosome, subunit 3 (ANAPC3) (132-208)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	100	100	1
rapC	Bacillus subtilis subsp. subtilis str. 168	57.368	99.738	0.572