Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   I8F58_RS11620 Genome accession   NZ_CP065773
Coordinates   2388409..2389779 (-) Length   456 a.a.
NCBI ID   WP_002328231.1    Uniprot ID   -
Organism   Enterococcus faecium strain 16-164     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2383409..2394779
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I8F58_RS11605 (I8F58_11615) epsC 2384730..2385266 (-) 537 WP_002287446.1 serine O-acetyltransferase EpsC -
  I8F58_RS11610 (I8F58_11620) gltX 2385588..2387048 (-) 1461 WP_002287447.1 glutamate--tRNA ligase -
  I8F58_RS11615 (I8F58_11625) - 2387218..2388375 (-) 1158 WP_002287448.1 PIN/TRAM domain-containing protein -
  I8F58_RS11620 (I8F58_11630) radA 2388409..2389779 (-) 1371 WP_002328231.1 DNA repair protein RadA Machinery gene
  I8F58_RS11625 (I8F58_11635) - 2389842..2390369 (-) 528 WP_002328232.1 hypothetical protein -
  I8F58_RS11630 (I8F58_11640) proB 2390600..2391406 (+) 807 WP_002287451.1 glutamate 5-kinase -
  I8F58_RS11635 (I8F58_11645) - 2391403..2392650 (+) 1248 WP_002298218.1 glutamate-5-semialdehyde dehydrogenase -
  I8F58_RS11640 (I8F58_11650) - 2392726..2393070 (+) 345 WP_002287507.1 hypothetical protein -
  I8F58_RS11645 (I8F58_11655) - 2393187..2393513 (-) 327 WP_002287453.1 hypothetical protein -
  I8F58_RS14055 - 2394128..2394652 (-) 525 WP_353803296.1 MFS transporter -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49917.57 Da        Isoelectric Point: 7.7416

>NTDB_id=514157 I8F58_RS11620 WP_002328231.1 2388409..2389779(-) (radA) [Enterococcus faecium strain 16-164]
MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTDRRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELN
RVLGGGVVPGSMVLIGGDPGIGKSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVTKEGSIAGPRMLEHMVDTVLYFE
GEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEVANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTVFG
NAKRTTTGLDFNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECFIGEIGLTG
EIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAETLKRVFR

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=514157 I8F58_RS11620 WP_002328231.1 2388409..2389779(-) (radA) [Enterococcus faecium strain 16-164]
ATGGCAAAAAAATCAAAAGTACAATTTGTTTGCCAAAATTGCGGGTATGTGTCGCCTAAGTTCTTGGGACGCTGCCCAAA
TTGCGGAAAATGGAATACGATGGTCGAAGAAATCGAACAAGATACAACGGACCGCAGAACTCGGACAAGTTTGACAGGAG
AAAAAGCAAAACCAACAAAAATCGCAGACGTCGTCCCTAAAAAAGAACCTCGGATCAAAACAAAATTAGAAGAACTGAAC
CGTGTGTTAGGCGGAGGTGTGGTGCCAGGGTCTATGGTCTTGATTGGTGGCGATCCAGGTATCGGGAAATCCACTTTGCT
GTTACAAGTTTCTCAGCAATTAGCAGCCATAGGCGGAAAAGTCTTATATGTATCCGGCGAAGAAAGCGCAGAGCAGATAA
AATTACGCGCGGAACGCCTCGGAAGTATCAACACAGAATTTTATCTATACGCTGAAACAGATATGAACGAAATCAGTCGT
GCCATCGAACATATCTCCCCTGATTATGTTATCATTGATTCCATCCAAACGATGACGCAGCCAGACATCACAAGTGTGGC
AGGAAGTGTCAGCCAAGTAAGAGAAACCACTGCAGAACTACTCAAAATCGCTAAAACAAATGGGATAGCCATTTTTATCG
TCGGTCATGTTACCAAAGAAGGCTCGATCGCCGGCCCAAGAATGTTAGAACATATGGTGGATACGGTGTTATATTTTGAA
GGTGAACAACATCATAGTTTTCGGATTTTGCGAGCAGTAAAAAACCGATTTGGCTCAACGAATGAGATTGGGATTTTTGA
AATGCATGAGCATGGATTGGAAGAAGTCGCTAATCCTTCACAGATTTTCTTAGAAGAACGCCTAGACGGTGCAACTGGCT
CTGCTATCGTTGTGGCAATGGAAGGGACACGTCCAATTTTGGTAGAGATCCAAGCATTAGTCACACCTACTGTGTTCGGA
AATGCCAAACGAACAACAACAGGTCTTGATTTCAATCGGGTATCTCTGATCATGGCAGTGCTGGAAAAAAGAGCTGGGTT
GCTTCTTCAGAATCAAGATGCTTACTTAAAAGCTGCAGGTGGGGTAAAATTAAATGAACCAGCGATTGATTTAGCTATCG
CTGTCAGTATTGCTTCAAGCTATAAAGAAAAAGGAACACAGCCAACGGAATGTTTCATCGGTGAAATTGGACTGACTGGA
GAAATCAGACGTGTCAGTCATATTGAACAACGAGTCAAAGAAGTCCAAAAACTCGGCTTCACAAAAGTATACCTACCTAA
AAACAACTTAGGTAACTGGGAAGCGCCAAAAGGAATCGAGATAGTTGGAGTAGCAACTTTGGCTGAAACGTTGAAGCGTG
TGTTCCGCTAA

Domains

Predicted by InterProScan.

AAA domain (AAA_25)

(69-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

74.066

99.781

0.739
  radA Streptococcus pneumoniae Rx1

74.066

99.781

0.739
  radA Streptococcus pneumoniae D39

74.066

99.781

0.739
  radA Streptococcus pneumoniae R6

74.066

99.781

0.739
  radA Streptococcus pneumoniae TIGR4

74.066

99.781

0.739
  radA Streptococcus mitis SK321

73.846

99.781

0.737
  radA Bacillus subtilis subsp. subtilis str. 168

67.406

98.904

0.667