Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   THEBA_RS01160 Genome accession   NC_017934
Coordinates   229945..230856 (-) Length   303 a.a.
NCBI ID   WP_014730106.1    Uniprot ID   I2F1Z0
Organism   Mesotoga prima MesG1.Ag.4.2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 224945..235856
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  THEBA_RS01130 (Theba_0202) sdaAB 225485..226153 (-) 669 WP_014730100.1 L-serine ammonia-lyase, iron-sulfur-dependent subunit beta -
  THEBA_RS01135 (Theba_0203) eno 226213..227496 (-) 1284 WP_014730101.1 phosphopyruvate hydratase -
  THEBA_RS01140 (Theba_0204) - 227511..228212 (-) 702 WP_014730102.1 uridine monophosphate kinase -
  THEBA_RS01145 (Theba_0205) tsf 228258..228863 (-) 606 WP_014730103.1 translation elongation factor Ts -
  THEBA_RS01150 (Theba_0206) coaD 228942..229418 (-) 477 WP_014730104.1 pantetheine-phosphate adenylyltransferase -
  THEBA_RS01155 (Theba_0207) - 229478..229942 (-) 465 WP_014730105.1 Rrf2 family transcriptional regulator -
  THEBA_RS01160 (Theba_0208) pilA 229945..230856 (-) 912 WP_014730106.1 signal recognition particle-docking protein FtsY Machinery gene
  THEBA_RS01165 (Theba_0209) - 230856..232598 (-) 1743 WP_014730107.1 proline--tRNA ligase -
  THEBA_RS01170 (Theba_0210) - 232719..233567 (+) 849 WP_014730108.1 purine-nucleoside phosphorylase -
  THEBA_RS01180 (Theba_0212) - 233831..235672 (+) 1842 WP_014730109.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 303 a.a.        Molecular weight: 32839.56 Da        Isoelectric Point: 5.0621

>NTDB_id=51383 THEBA_RS01160 WP_014730106.1 229945..230856(-) (pilA) [Mesotoga prima MesG1.Ag.4.2]
MGVFSRLNDGLKRTREGFFKRVRKLLSFESLDSDSLEEIEELLILSDMGSETAEEVIEALKDRKDKGKEPIEVLKEVLVE
ILDIPFEVPQARPYVVSVVGVNGTGKTTTIGKLSKLYSSNGNKVVLAACDTFRAAAVEQLRIWSERANVDFISQGQGADS
AAVAFDAVRHAVSKGKDVVIVDTAGRLHTRSNLMEELKKVHRVIGKASPGAPNEVLLVIDATTGQNGLVQAKKFKEAVDV
TGIVLTKLDGTAKGGIIFAIVKELGIPVRYIGVGEKEDDLKPFDSREFVNALLSTGEEGSEEA

Nucleotide


Download         Length: 912 bp        

>NTDB_id=51383 THEBA_RS01160 WP_014730106.1 229945..230856(-) (pilA) [Mesotoga prima MesG1.Ag.4.2]
ATGGGAGTTTTTTCTAGACTTAACGATGGTTTGAAGAGGACCAGAGAAGGCTTCTTCAAGAGGGTAAGAAAACTTCTCTC
TTTTGAATCGCTTGATTCTGACTCACTTGAAGAAATCGAGGAGCTCCTGATATTGTCCGATATGGGTTCCGAAACGGCTG
AGGAAGTCATCGAAGCTTTGAAAGATAGAAAAGATAAAGGGAAGGAACCCATTGAGGTACTGAAAGAAGTCCTTGTCGAG
ATACTTGACATACCCTTTGAGGTGCCACAAGCGAGACCTTACGTAGTTTCGGTGGTCGGCGTTAACGGCACCGGAAAGAC
AACCACAATAGGCAAGCTTTCCAAACTCTACTCGAGCAATGGAAACAAAGTGGTTCTCGCTGCCTGTGACACTTTTAGGG
CAGCCGCAGTAGAACAACTAAGAATATGGAGTGAAAGAGCGAACGTTGACTTCATAAGCCAGGGTCAGGGCGCCGATTCC
GCAGCAGTGGCCTTTGACGCTGTAAGGCACGCAGTAAGTAAGGGAAAGGATGTAGTGATTGTCGACACGGCAGGCAGATT
GCACACCCGAAGCAACCTGATGGAAGAACTGAAGAAAGTACATAGAGTAATCGGCAAAGCATCGCCCGGAGCTCCTAATG
AAGTATTGCTTGTAATTGATGCAACTACGGGACAGAATGGCCTGGTACAGGCCAAAAAATTCAAAGAAGCTGTCGATGTC
ACCGGGATCGTACTGACGAAGCTTGATGGTACCGCTAAAGGTGGTATTATTTTCGCTATTGTAAAGGAACTTGGGATACC
GGTGAGATATATAGGCGTAGGAGAAAAGGAAGATGATTTGAAACCCTTTGACAGCAGGGAATTCGTGAACGCTCTTTTGT
CTACCGGGGAAGAGGGTTCAGAGGAGGCTTAG

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(21-81)

SRP54-type protein, GTPase domain (SRP54)

(94-293)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I2F1Z0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

49.175

100

0.492

Multiple sequence alignment