Detailed information NTG0513829

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: I6G54_RS11695
• Genome accession: NZ_CP065743
• Coordinates: 2029066..2032791 (+)
• Length: 1241 a.a.
• NCBI ID: WP_002194449.1
• Uniprot ID: -
• Organism: Bacillus tropicus strain FDAARGOS_897
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 2024066..2037791

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6G54_RS11680 (I6G54_11680)	-	2024224..2024817 (+)	594	WP_000347516.1	TVP38/TMEM64 family protein	-
I6G54_RS11685 (I6G54_11685)	lepB	2024874..2025437 (+)	564	WP_002194447.1	signal peptidase I	-
I6G54_RS11690 (I6G54_11690)	addB	2025554..2029069 (+)	3516	WP_002194448.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
I6G54_RS11695 (I6G54_11695)	addA	2029066..2032791 (+)	3726	WP_002194449.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
I6G54_RS11700 (I6G54_11700)	-	2032804..2033091 (+)	288	WP_002194451.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
I6G54_RS11705 (I6G54_11705)	gerPF	2033229..2033444 (-)	216	WP_001141566.1	spore germination protein GerPF	-
I6G54_RS11710 (I6G54_11710)	-	2033487..2033873 (-)	387	WP_002194453.1	spore germination protein GerPE	-
I6G54_RS11715 (I6G54_11715)	gerPD	2033889..2034083 (-)	195	WP_001102341.1	spore germination protein GerPD	-
I6G54_RS11720 (I6G54_11720)	gerPC	2034090..2034704 (-)	615	WP_002194454.1	spore germination protein GerPC	-
I6G54_RS11725 (I6G54_11725)	gerPB	2034773..2034979 (-)	207	WP_001012508.1	spore germination protein GerPB	-
I6G54_RS11730 (I6G54_11730)	gerPA	2034994..2035215 (-)	222	WP_001111187.1	spore germination protein GerPA	-
I6G54_RS11735 (I6G54_11735)	-	2035312..2035491 (-)	180	WP_000462845.1	aspartyl-phosphate phosphatase Spo0E family protein	-
I6G54_RS11740 (I6G54_11740)	-	2035739..2036638 (+)	900	WP_038356988.1	fumarylacetoacetate hydrolase family protein	-
I6G54_RS11745 (I6G54_11745)	-	2036674..2036958 (-)	285	WP_002194464.1	hypothetical protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142703.02 Da
• Isoelectric Point: 4.8669

>NTDB_id=513829 I6G54_RS11695 WP_002194449.1 2029066..2032791(+) (addA) [Bacillus tropicus strain FDAARGOS_897]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLINEPGSQHIRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNTIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEQHI
RKATELAMLPDGPAPRVETLQADLVLLGTLSSAARESWTSVYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFQDMHPVLEKLVQLVKVFTERFQTMKRDKGMVDFTDLEHFCLQILSEQSESGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGATYPEGEDVAAELLCIQQTEEEVIDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEKELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEILLELGQGSIPDEIYGYSASWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLQSERKEEVYDRLMWKYGYEEATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIRTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITIEVLQEQIAGMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGESGESILVQGVIDCMIEEEDGITLIDFKTDTIEGKFSGGFEQAKPIL
EERYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVKIEE

Nucleotide

• Length: 3726 bp

>NTDB_id=513829 I6G54_RS11695 WP_002194449.1 2029066..2032791(+) (addA) [Bacillus tropicus strain FDAARGOS_897]
ATGATAGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAGTGGACAGATGACCAGTGGAAAGCGGTTGTAGCGAACGGACG
TGATATTTTAGTCGCGGCAGCAGCTGGTTCAGGGAAAACAGCGGTATTAGTTGAACGTATTATTAAAAAGATTATAAATG
AAGAAAACCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAGATGAAAAACAGAATT
GGGGAAGCGTTAGAAAAAGTATTAATTAATGAGCCGGGCTCTCAGCACATTAGAAAGCAGCTGAGCTTATTAAATAAAGC
TTCCATTTCAACGATCCATTCATTTTGTTTACAAGTTATTAGAGGATACTATTACATGCTTGATGTTGATCCTCGTTTCC
GCATAGCGAATCAAACAGAAAATGAACTATTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAGTATGGAATTGAA
GATAATACGATATTCTTTGAACTTGTTGATCGTTATACGAGTGACCGTAGTGACGATGATTTACAACGTATGATTTTAGC
ACTTCATACAGAATCAAGAGCACATCCAAATCCGGAAAAATGGCTTGATAAATTAGTAGAAGCATACGATGTGGAAGGAA
AGACAATTGAAGATTTAGTGTACGCTTCCTACTTATTAGAAGATGTGAAATTCCAACTTGAAACAGCGGAACAGCATATT
CGTAAAGCGACAGAGCTCGCAATGCTTCCTGATGGTCCAGCGCCTCGCGTTGAAACCCTGCAAGCAGATTTAGTTTTACT
TGGAACGTTATCATCAGCTGCTCGTGAATCGTGGACAAGCGTGTATGAAGCGATGCAAAACGTATCGTGGCAAACGTTAA
AGCGTATTAAGAAAAGTGATTACAACGAGGATGTTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAAAAATTACAAGAAGAGCTATTTAGCCGTAAGCCTGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAGCTTGTTCAGCTCGTAAAAGTATTTACCGAGCGTTTCCAAACGATGAAGCGAGATAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAACAAAGTGAAAGTGGTGAAATGAAGCCATCAGCAGTAGCGCTTCAA
TATCGCAATAAATTTGCTGAAGTACTAGTCGATGAATATCAAGATACGAACTTCGTACAAGAATCGATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTGTTCATGGTTGGCGACGTGAAGCAGTCGATCTATCGTTTCCGACTAGCAG
AACCAGGATTATTCCTAGGAAAGTATAAACGCTTCACGCAAGAAGGATTAGGCGGCGGAATGAAGATCGATTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTGTTAGCAGGCACGAACTTTATCTTCAAACAAATTATGGGCGAAGAAGTCGGAGAAAT
CGATTACGATGCTGACGCTGAATTAAAGCTAGGTGCTACCTATCCAGAAGGTGAAGATGTAGCGGCTGAACTACTATGCA
TTCAGCAAACGGAAGAAGAGGTAATAGACGGTGAAGAAGGCGCAGAAGTAGAAAAGGCACAGCTTGAAGCTCGTCTTATG
GCGCAGCGTATTAAAGCGATGGTTGATTCTGGTTATGAAGTGTATGATCGTAAAACGGATAGTATGCGTCCTGTACAATA
CCGCGACTTCGTTATTTTGCTCCGCTCCATGCCGTGGGCACCGCAAATTATGGAAGAGTTGAAATTACAAGGAATTCCAG
TATACGCTGATCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTTATCGATAAT
CCGATGCAAGATATTCCGCTTGCAGCCGTGCTTCGTTCCCCAATCGTTGGATTAAATGATGAAGAACTTGCAACACTTCG
TGCTCATGGGAAGAAAGGTTCGTTTTATGAAGTAATGAGCTCATTCTTAAAAGGCGCACCGCTTGAAGAAGAAAAAGAAC
TACATGATAAATTAGAATGGTTCTACAACTTACTGCAAGGATGGCGTGAATTCGCGCGCCAACAGTCTCTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGGTATTACGACTTTGTCGGTGGTTTACCAGCTGGAAAGCAAAGGCAAGCAAACTT
GCGTGTACTATATGACCGCGCAAGACAATATGAAGCAACATCGTTTAGAGGACTATTCCGCTTCTTACGCTTTATTGAGC
GTATTTTAGAACGCGGTGATGATATGGGGACGGCAAGAGCTTTAGGTGAACAAGAAGATGTTGTTCGCATTATGACAATT
CATAAAAGTAAAGGACTTGAGTTCCCAGTCGTATTCGTAGCTGGACTAGGTCGTCGCTTTAATACACAAGACTTAATGAA
ACGTTTCTTACTGCATAAAGACTTCGGCTTCGGTTCGCAATTTATTGATCCACGTAAACGAATTAAATATACGACATTAT
CGCAACTTGCGATTAAGCGTAAAATGAAAATGGAATTAATTGCGGAAGAAATGCGCGTATTATATGTTGCGTTAACACGT
GCAAAAGAGAAGTTAATTTTAATTGGAACGGTTAAGGATGCAAATAAAGAAATGGAAAAATGGCTTGATGCGAGGGAGCA
TAGTGAATGGTTATTACCAGATCATATACGTGCCGGAGCGTCTTGCTACTTAGACTGGATTGCACCTTCATTATATAGAC
ACCGTGATAGTGAAATACTTCTTGAATTGGGACAAGGAAGCATTCCAGATGAAATTTATGGGTATAGTGCAAGCTGGAAA
GTAGAAGTTGTTGACGGAAACACGTTACTTGCGCCAGAACCAGTTCAAGAAGAGAAGCAAGAGTTGTTAGAAGCACTTCG
TGAGAAAAAGGCCGTTCCTCTGCAAAGTGAACGGAAAGAAGAGGTGTACGATAGATTAATGTGGAAGTACGGATATGAGG
AAGCGACATCTCATCGTGCGAAGCAGTCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAATGCC
TTTATTAAAAAATTACGTGCACCAATTAGAACACGTCCTCGTTTTATGGAGAAAAAAGGTTTAACGTACGCAGAGCGTGG
AACCGCAGTCCATGCCGTTATGCAGCATGTTGATTTGAAGAAGCCGATTACGATTGAAGTTCTTCAAGAGCAAATTGCTG
GAATGGTAAATAAGGAATTATTAACATTTGAACAAGCAGAAGAAATAGCGATTGAAAAAGTAATTTCATTCTTTGACAGT
GACCTAGGTAAAAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGAAGC
ATATCAAGATTGGCAAGGGGAGAGCGGGGAATCCATTCTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGATG
GCATTACGTTAATCGACTTCAAAACAGATACGATTGAAGGGAAATTCTCAGGTGGATTCGAACAAGCGAAACCAATTTTA
GAAGAGCGATATAAAGTGCAGCTTTCGTTATATGCAAAAGCGCTCGAGAAAAGCTTACAACATCCTGTGAAAGAGAAATG
TTTATACTTCTTTGATGGTAATCATGTTGTAAAAATCGAAGAATAG

Domains

Predicted by InterproScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1059-1231)

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.908	100	0.539