Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   I6G77_RS25660 Genome accession   NZ_CP065739
Coordinates   4986575..4990090 (+) Length   1171 a.a.
NCBI ID   WP_042515580.1    Uniprot ID   -
Organism   Bacillus tropicus strain FDAARGOS_920     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4981575..4995090
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6G77_RS25625 (I6G77_25625) cspA 4982395..4982598 (+) 204 WP_000301518.1 RNA chaperone/antiterminator CspA -
  I6G77_RS25630 (I6G77_25630) - 4982937..4983155 (-) 219 WP_033691165.1 hypothetical protein -
  I6G77_RS25635 (I6G77_25635) - 4983477..4983662 (-) 186 WP_002194444.1 hypothetical protein -
  I6G77_RS25640 (I6G77_25640) - 4983943..4984524 (+) 582 WP_000444420.1 competence protein ComK -
  I6G77_RS25645 (I6G77_25645) - 4984595..4984894 (-) 300 WP_000655757.1 hypothetical protein -
  I6G77_RS25650 (I6G77_25650) - 4985245..4985838 (+) 594 WP_000347516.1 TVP38/TMEM64 family protein -
  I6G77_RS25655 (I6G77_25655) lepB 4985895..4986458 (+) 564 WP_002194447.1 signal peptidase I -
  I6G77_RS25660 (I6G77_25660) addB 4986575..4990090 (+) 3516 WP_042515580.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  I6G77_RS25665 (I6G77_25665) addA 4990087..4993812 (+) 3726 WP_002194449.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  I6G77_RS25670 (I6G77_25670) - 4993825..4994112 (+) 288 WP_002194451.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  I6G77_RS25675 (I6G77_25675) gerPF 4994250..4994465 (-) 216 WP_001141566.1 spore germination protein GerPF -
  I6G77_RS25680 (I6G77_25680) - 4994508..4994894 (-) 387 WP_002194453.1 spore germination protein GerPE -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134168.24 Da        Isoelectric Point: 5.2122

>NTDB_id=513798 I6G77_RS25660 WP_042515580.1 4986575..4990090(+) (addB) [Bacillus tropicus strain FDAARGOS_920]
MSLRFVIGRAGSGKSTLCLHEVQEELKQRPRGETILYLVPEQMTFQTQQALIGSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGGMIAEFKRYNVTPSNVYEMWQQLDAHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLVEKLPQSEYVKGAEIYIDGFHSFSPQELEIVRQLMVCGARVTITLTIDE
KTLAQPVNELDLFYETTLTYEKIKQVAREEKIEIEKTIPLMEQPRFHSPALAHLETYYEARPNEKFHGEASVAIRTAANL
RAEVEGVAREIRRLVADENYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKETMREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDTNGMITDSEREMEEKINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEALRIRAEESGDFLFATDHEQVWEEVMSLLDTFVEMLGEEK
MSLSMFTDVMSTGLEALQFANIPPSLDQVLIANIDRSRLSNVKATFVIGVNEGVIPAAPMDEGMLSDEERDVLSAAGIEL
APTTRQTLLEEQFVMYQMVTRATEKLYISCPLADEEGKTLLASSFIKKIKRMFPDVKDTFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAQKLSTAVSRDLYGDKIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKRIADRLLRENRTWADLSIKECEHLSAVVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSIILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFSLPNGVKMEVVGRIDRVDKAEDENG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTSNAHTWMKKGGTASPAGVLYFHIHNPIVEVNGDASEAEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHTFENIGKDITEGVIDIAP
YKKGNKAACTFCNFKSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=513798 I6G77_RS25660 WP_042515580.1 4986575..4990090(+) (addB) [Bacillus tropicus strain FDAARGOS_920]
ATGTCACTTCGATTTGTGATTGGCAGAGCGGGAAGTGGAAAAAGTACACTTTGTTTACACGAAGTGCAAGAAGAGTTAAA
GCAGCGTCCAAGAGGGGAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAAACGCAGCAGGCGTTAATTGGCA
GTGAGGATGTTAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCACGATTAGCGTGGAAAGTATTGCAAGAAGTTGGC
GGAGCGAGTCGTCTTCACATTGATGAAGCGGGCGTACATATGTTACTGCGTAAAATTGTAGAGTCTCGTAAAGATGGATT
ATCGGTGTTCCAAAAAGCAGCGGAACAAAACGGTTTCTTTGAACATCTTGGCGGTATGATTGCGGAGTTTAAACGATATA
ACGTGACGCCATCAAACGTATATGAAATGTGGCAACAATTAGATGCGCATAGTAGCAGTGCTGAACAAAAGCTATTAGCG
AATAAAGTATATGATTTACAGCTACTATATGATGATTTTGAGCGTGCTTTAATCGGAAAATATTTAGATTCAGAAGACTA
CCTGCAATTGCTAGTCGAAAAGCTTCCGCAGTCTGAATATGTAAAGGGAGCTGAAATTTATATAGATGGATTTCATTCAT
TCTCACCGCAAGAGCTAGAAATTGTAAGGCAGCTTATGGTTTGCGGAGCGAGGGTTACAATCACGTTAACGATAGATGAA
AAAACGTTAGCACAGCCGGTAAATGAACTAGATTTATTTTATGAAACGACATTAACGTATGAAAAAATAAAACAAGTAGC
ACGTGAAGAGAAGATAGAAATTGAAAAAACGATTCCACTTATGGAACAGCCGCGTTTTCATTCTCCGGCGCTAGCGCATT
TAGAAACGTACTATGAAGCACGTCCAAATGAAAAATTTCACGGGGAAGCAAGTGTAGCGATTCGTACAGCTGCGAATTTA
CGAGCTGAAGTAGAAGGTGTTGCTCGTGAAATTCGTAGACTTGTGGCGGACGAAAACTATCGTTACCGAGATATTGCTGT
TCTTCTTCGTAACGGGGAAAGTTATTACGATGTAATGCGGACGCTATTTACAGATTATAATATCCCGCACTTCATCGATG
AAAAACGCCCGATGTCACATCATCCGCTAGTAGAATGCATTCGTTCTGCACTCGAGATTATTAGTGGGAATTGGCGTTAT
GATGCAGTATTTCGCTGCGTGAAAACAGAGCTTTTATATCCATTAGACGTAAGAAAAGAAACGATGCGTGAAGAGATGGA
TGAGTTTGAAAACTACTGTTTAGCGTACGGTGTACAAGGGAAGAGATGGACTTCTGAGGATCCGTGGATGTATCGCCGCT
ATCGTTCTCTTGACGATACAAACGGGATGATTACAGACAGTGAACGTGAAATGGAAGAGAAAATAAATCGATTGCGTGAC
GTTGTAAGAACGCCAGTTATTCGTATGCAAAAAAGACTGAAGCGTGCGGGAACAGTTATGCAAATGTGCGAGGCTGTTTA
CTTATTTTTAGAAGAGCTGGACGTTCCGAAAAAATTAGAAGCATTACGTATTCGTGCAGAAGAGAGTGGAGATTTCTTAT
TTGCGACAGATCATGAACAAGTATGGGAAGAAGTTATGAGTCTTCTTGATACGTTCGTTGAGATGCTTGGCGAAGAAAAA
ATGTCACTTTCTATGTTCACAGACGTTATGTCGACAGGTCTTGAGGCGCTTCAATTCGCAAACATTCCGCCGTCATTAGA
CCAAGTGTTAATTGCGAATATTGATCGTTCTAGATTATCAAATGTGAAAGCAACATTTGTCATTGGCGTGAATGAAGGTG
TGATTCCAGCAGCACCTATGGATGAAGGTATGCTTTCTGATGAGGAAAGAGATGTTCTTAGCGCTGCAGGTATTGAACTC
GCACCAACGACGAGACAAACTTTATTAGAAGAACAGTTCGTTATGTACCAAATGGTAACGAGAGCAACTGAGAAATTATA
TATTTCATGTCCGCTTGCAGATGAGGAAGGGAAGACGTTACTTGCGTCTAGCTTTATTAAGAAAATAAAAAGAATGTTCC
CTGATGTGAAAGATACATTTATTACGAATGACGTAAATGATTTATCACGTTCGGAACAAATTTCGTACGTAGCAACGCCA
GAAGTAACGTTGTCATATGTTATGCAGCAACTTCAAACGTGGAAGCGATATGGATTTGAAGGGAATTTAGACTTTTGGTG
GGACGTATATAATTTCTACGTTACTTCAGATGAATGGAAGCAAAAAAGTAGCCGCGTATTATCAAGCTTATTCTACCGAA
ATCGTGCGCAAAAGCTAAGTACAGCGGTAAGTAGAGATTTATACGGAGATAAAATAAAAGGAAGCGTTTCTCGTATGGAA
TTATTTAACCGTTGTGCGTACGCTCATTTCGCGCAACACGGTTTATCGTTAAGAGAGCGTGATATTTTCAAGCTTGATGC
GCCAGATATCGGAGAGCTCTTCCATGCAGCGCTGAAGAGAATTGCAGACAGGCTATTACGTGAAAACCGTACTTGGGCGG
ATTTATCAATAAAAGAGTGTGAGCATCTTTCTGCTGTAGTAATAGAAGAGATTGCACCGTTATTACAAAGACAAATTTTA
TTAAGTTCAAACCGTCACTTCTATTTAAAACAAAAACTACAACAAATCATTTTCCGTACGTCAATCATTCTTCGTGAACA
TGCGAAGTCTAGCGGTTTCGTACCAGTTGATCTAGAAGTGCCATTTGGTATGGGCGGTACAGGATCACTTCCGCCAATGG
AATTCTCCTTACCTAATGGTGTAAAGATGGAAGTAGTTGGCCGTATTGACCGTGTTGATAAGGCAGAAGATGAAAACGGA
ACATTCCTACGTATTATTGACTATAAATCAAGCTCAAAAGCGTTAGACTTAACGGAAGTGTATTACGGATTAGCACTTCA
AATGTTAACATATTTAGATGTTGTTACTTCAAATGCACATACGTGGATGAAAAAAGGCGGCACAGCATCACCAGCTGGTG
TACTGTATTTCCACATTCATAACCCAATTGTTGAGGTAAATGGTGACGCATCTGAAGCAGAAATTGAAAAGGAAATTTTA
AAGAAATTTAAAATGAAAGGGCTCGTACTAGGAGATGCTGATGTTGTTCGTTTAATGGATAACAAACTTTCAACAGGAAG
CTCAGATATTATTTCTGCTGGTCTGAAAAAAGACGGTAGCTTTAGTGCGCGTTCAAGTATTGCCAGTGAACAAGAGTTTA
ACGTCCTGCAAAAATACGTACACCATACGTTTGAAAATATCGGGAAAGACATTACAGAAGGCGTTATTGATATTGCTCCT
TACAAAAAGGGGAATAAAGCGGCATGTACGTTCTGTAACTTCAAATCTGTTTGTCAGTTTGATGAATCACTTGAAGATAA
CCAATTCCGTACGCTAAAAGATATGAAAGATAGCGAAGCGATGGAGAAAATTAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(305-669)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.232

100

0.493