Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   I6G77_RS12370 Genome accession   NZ_CP065739
Coordinates   2423810..2424244 (-) Length   144 a.a.
NCBI ID   WP_000811492.1    Uniprot ID   A0A0G8EA73
Organism   Bacillus tropicus strain FDAARGOS_920     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2418810..2429244
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6G77_RS12350 (I6G77_12350) - 2419242..2419799 (+) 558 WP_033691464.1 PadR family transcriptional regulator -
  I6G77_RS12355 (I6G77_12355) - 2419981..2420412 (-) 432 WP_002194049.1 NUDIX hydrolase -
  I6G77_RS12360 (I6G77_12360) metE 2421059..2423347 (+) 2289 WP_002194050.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  I6G77_RS12365 (I6G77_12365) comJ 2423385..2423774 (-) 390 WP_002194051.1 competence protein ComJ -
  I6G77_RS12370 (I6G77_12370) nucA/comI 2423810..2424244 (-) 435 WP_000811492.1 DNA-entry nuclease Machinery gene
  I6G77_RS12375 (I6G77_12375) - 2424481..2425230 (+) 750 WP_000388464.1 DUF3967 domain-containing protein -
  I6G77_RS12380 (I6G77_12380) kinB 2425284..2426558 (-) 1275 WP_042514717.1 sporulation sensor histidine kinase KinB -
  I6G77_RS12385 (I6G77_12385) - 2426868..2427068 (+) 201 WP_000929285.1 hypothetical protein -
  I6G77_RS12390 (I6G77_12390) - 2427192..2428349 (+) 1158 WP_000434566.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16012.35 Da        Isoelectric Point: 8.4331

>NTDB_id=513769 I6G77_RS12370 WP_000811492.1 2423810..2424244(-) (nucA/comI) [Bacillus tropicus strain FDAARGOS_920]
MKQLKGIIISIIAILSILVAVYEVLVPEETSTKKTNAYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFDVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=513769 I6G77_RS12370 WP_000811492.1 2423810..2424244(-) (nucA/comI) [Bacillus tropicus strain FDAARGOS_920]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTATTTTAGTAGCGGTTTATGAAGTACTTGTTCC
AGAGGAAACAAGTACAAAAAAAACAAATGCGTATGATCAAGTTCTAGAATTTCCGAAAGAGAGATATCCAGAAACAGGGA
AACATATTACGGATGCGATAAAGGAAGGACATTCAGAAGTTTGTACAATCGACCGTGGTGGCGCTGCGGATAGAAGAAAG
TTATCGTTAGCTCCATACCCATCAAAAAAAGGGTATGATCGCGATGAATGGCCAATGGCGATGTGTAAAGAAGGTGGAAA
AGGAGCACATATTGAATATATAAGTCCAGCCGATAATCGCGGAGCAGGTTCTTGGGTAGGAAATAAGTTAGATAAATACC
CAGATGGTACGCGTGTGAAATTTGACGTAAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0G8EA73

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

70.796

78.472

0.556