Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   I6G56_RS13145 Genome accession   NZ_CP065686
Coordinates   2595035..2596411 (-) Length   458 a.a.
NCBI ID   WP_006026043.1    Uniprot ID   A0AAU8SNN7
Organism   Burkholderia humptydooensis strain FDAARGOS_899     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2590035..2601411
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6G56_RS13125 (I6G56_13115) cls 2590516..2591955 (+) 1440 WP_006026047.1 cardiolipin synthase -
  I6G56_RS13130 (I6G56_13120) - 2591986..2592465 (-) 480 WP_006026046.1 glutathione peroxidase -
  I6G56_RS13135 (I6G56_13125) - 2592612..2594552 (+) 1941 WP_006026045.1 ATP-binding cassette domain-containing protein -
  I6G56_RS13140 (I6G56_13130) - 2594605..2594871 (-) 267 WP_009912854.1 DUF2866 domain-containing protein -
  I6G56_RS13145 (I6G56_13135) radA 2595035..2596411 (-) 1377 WP_006026043.1 DNA repair protein RadA Machinery gene
  I6G56_RS13150 (I6G56_13140) alr 2596422..2597492 (-) 1071 WP_006026042.1 alanine racemase -
  I6G56_RS13155 (I6G56_13145) lplT 2597701..2599008 (+) 1308 WP_006026041.1 lysophospholipid transporter LplT -
  I6G56_RS13160 (I6G56_13150) thiD 2599097..2599906 (+) 810 WP_006026040.1 bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase -
  I6G56_RS13165 (I6G56_13155) - 2600323..2601300 (-) 978 WP_043282419.1 DUF1853 family protein -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 48758.21 Da        Isoelectric Point: 7.2044

>NTDB_id=512962 I6G56_RS13145 WP_006026043.1 2595035..2596411(-) (radA) [Burkholderia humptydooensis strain FDAARGOS_899]
MAKQKTVFVCTECGGQTPKWQGQCPSCHAWNTLVESVESAPSAHRFQSLAKRAPVQRLADIEAADVPRFSTGIGEFDRVL
GGGLVAGGVVLIGGDPGIGKSTLLLQSLAQIASERPALYISGEESGAQIALRAQRLALLEGGASAADLKLLAEIQLEKIQ
ATLDAERPDIAVIDSIQTIYSEALTSAPGSVAQVRECAAQLTRIAKQSGTAIIMVGHVTKEGNLAGPRVLEHIVDTVLYF
EGDTHSSFRLVRAFKNRFGAVNELGVFAMTERGLRGVANPSALFLSQHTEVVPGSCVLVTQEGTRPLLVEVQALVDTANV
PNPRRLAVGLEQNRLAMLLAVLHRHAGIACFDQDVFLNAVGGVKITEPAADLAVLLAIHSSMRNKPLPKGLIVFGEVGLA
GEIRPSPRGQERLREAAKLGFTTALIPKANAPKQPIDGLRVHAVERIEQAIDQIRVLE

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=512962 I6G56_RS13145 WP_006026043.1 2595035..2596411(-) (radA) [Burkholderia humptydooensis strain FDAARGOS_899]
ATGGCGAAGCAGAAGACGGTATTCGTCTGCACCGAGTGCGGCGGGCAGACGCCGAAGTGGCAGGGGCAATGCCCGTCGTG
CCACGCGTGGAACACCCTCGTCGAATCGGTCGAGAGCGCGCCGTCCGCGCACCGTTTCCAGTCGCTCGCGAAGCGCGCGC
CCGTGCAGCGCCTCGCCGACATCGAGGCGGCCGACGTGCCGCGCTTCTCGACCGGGATCGGCGAGTTCGACCGGGTGCTG
GGAGGCGGGCTCGTCGCGGGCGGCGTCGTGCTGATCGGCGGCGATCCGGGGATCGGCAAGTCGACGCTGCTGCTGCAGTC
GCTCGCGCAGATCGCGAGCGAGCGGCCGGCGCTCTATATCAGCGGCGAGGAATCGGGCGCGCAGATCGCGCTGCGCGCGC
AGCGGCTCGCGCTCCTCGAAGGCGGCGCGAGCGCGGCCGACCTGAAGCTCCTCGCCGAGATCCAGCTCGAGAAGATCCAG
GCGACGCTCGACGCCGAGCGGCCCGACATCGCGGTCATCGACTCGATCCAGACGATCTACTCGGAAGCGTTGACGTCGGC
GCCGGGCTCGGTCGCGCAAGTGCGTGAATGCGCGGCGCAATTGACGCGCATCGCGAAGCAATCGGGCACGGCGATCATCA
TGGTCGGCCACGTGACGAAAGAGGGCAACCTCGCGGGGCCGCGCGTGCTCGAGCACATCGTCGACACCGTGCTGTACTTC
GAAGGCGACACGCATTCGTCGTTCCGGCTCGTGCGCGCGTTCAAGAACCGCTTCGGCGCGGTCAACGAGCTCGGCGTGTT
CGCGATGACCGAGCGCGGCCTGCGCGGCGTCGCGAATCCGTCCGCGCTGTTCCTGTCGCAGCACACGGAGGTCGTGCCCG
GCTCGTGCGTGCTCGTCACGCAGGAGGGCACGCGGCCGCTGCTCGTCGAGGTGCAGGCGCTCGTCGATACCGCGAACGTG
CCGAACCCGCGCCGGCTCGCGGTCGGCCTCGAACAGAACCGGCTCGCGATGCTGCTCGCCGTGCTGCACCGGCACGCGGG
CATCGCGTGCTTCGATCAGGACGTGTTCCTGAATGCCGTCGGCGGCGTAAAGATCACCGAGCCGGCTGCCGATCTTGCGG
TGCTGCTCGCGATCCACTCGTCGATGCGTAACAAACCGTTGCCAAAGGGTCTGATCGTATTCGGCGAAGTCGGGCTCGCG
GGCGAGATCCGGCCGTCGCCGCGCGGGCAGGAGCGCCTGCGCGAGGCGGCGAAGCTTGGCTTCACGACCGCGCTGATTCC
GAAGGCGAATGCGCCGAAACAGCCGATCGACGGGCTTCGCGTGCATGCGGTCGAGCGGATCGAGCAGGCGATCGACCAGA
TCCGCGTGCTCGAATGA

Domains

Predicted by InterproScan.

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(363-452)

AAA domain (AAA_25)

(76-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50.913

95.633

0.487
  radA Streptococcus mitis NCTC 12261

47.702

99.782

0.476
  radA Streptococcus pneumoniae Rx1

50

93.886

0.469
  radA Streptococcus pneumoniae R6

50

93.886

0.469
  radA Streptococcus pneumoniae TIGR4

50

93.886

0.469
  radA Streptococcus pneumoniae D39

50

93.886

0.469
  radA Streptococcus mitis SK321

50

93.886

0.469