Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   I6G56_RS04060 Genome accession   NZ_CP065686
Coordinates   564879..566387 (-) Length   502 a.a.
NCBI ID   WP_006024494.1    Uniprot ID   A0AAU8SQX0
Organism   Burkholderia humptydooensis strain FDAARGOS_899     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 559879..571387
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6G56_RS04035 (I6G56_04025) - 560907..561539 (-) 633 WP_009911186.1 LON peptidase substrate-binding domain-containing protein -
  I6G56_RS04040 (I6G56_04030) rapZ 561566..562459 (-) 894 WP_006024491.1 RNase adapter RapZ -
  I6G56_RS04045 (I6G56_04035) hprK 562536..563504 (-) 969 WP_004195225.1 HPr(Ser) kinase/phosphatase -
  I6G56_RS04050 (I6G56_04040) ptsN 563628..564137 (-) 510 WP_004534308.1 PTS IIA-like nitrogen regulatory protein PtsN -
  I6G56_RS04055 (I6G56_04045) hpf 564370..564729 (-) 360 WP_006024493.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  I6G56_RS04060 (I6G56_04050) rpoN/rpoN1 564879..566387 (-) 1509 WP_006024494.1 RNA polymerase factor sigma-54 Machinery gene
  I6G56_RS04065 (I6G56_04055) lptB 566556..567332 (-) 777 WP_006024495.1 LPS export ABC transporter ATP-binding protein -
  I6G56_RS04070 (I6G56_04060) lptA 567329..567994 (-) 666 WP_006024496.1 lipopolysaccharide transport periplasmic protein LptA -
  I6G56_RS04075 (I6G56_04065) lptC 568013..568627 (-) 615 WP_006024497.1 LPS export ABC transporter periplasmic protein LptC -
  I6G56_RS04080 (I6G56_04070) - 568632..569171 (-) 540 WP_009911188.1 KdsC family phosphatase -
  I6G56_RS04085 (I6G56_04075) kdsD 569171..570154 (-) 984 WP_006024499.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 55162.29 Da        Isoelectric Point: 4.8337

>NTDB_id=512954 I6G56_RS04060 WP_006024494.1 564879..566387(-) (rpoN/rpoN1) [Burkholderia humptydooensis strain FDAARGOS_899]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVATAIAQNPLLESDDDWIASPLRVAADGSVIAQTPAPSAPEPMA
GASNGTTATNERTERDEAAGVDEYDSYSADSGDANQWNLDDFGRSPAASDDDDLPPMQIHETSTSLRDHLMAQLRVTQAS
PRDRALVTFLIESLDDDGYLTASLEEVLADLPEELEVDVDELNAALALVHSFDPAGVGARSASECLKLQLLRLDPSPTRT
LALDIVSQHLELLAARDFTRLRKQLKATDDELRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKTGQNWQAELNPEVV
PKLRINHLYANILRNSRGDPSTGSLKQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTL
GLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVA
RRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=512954 I6G56_RS04060 WP_006024494.1 564879..566387(-) (rpoN/rpoN1) [Burkholderia humptydooensis strain FDAARGOS_899]
ATGAAAGCCAGTCTCCAACTTCGCCTGTCGCAGCATCTCGCGCTCACGCCCCAGCTTCAGCAGTCGATCCGGCTGCTGCA
GTTGTCGACGCTCGAACTGCAGCAGGAAGTCGCGACGGCGATCGCGCAGAACCCGCTGCTCGAAAGCGACGACGACTGGA
TCGCGAGCCCGTTGCGCGTCGCGGCCGACGGCTCGGTGATCGCGCAGACGCCCGCGCCGTCCGCGCCCGAGCCGATGGCG
GGCGCGTCGAACGGCACGACTGCGACGAATGAGCGCACCGAGCGCGACGAAGCCGCCGGCGTCGACGAATACGACAGCTA
TTCGGCCGACAGCGGCGACGCGAACCAGTGGAACCTCGACGATTTCGGCCGCTCGCCCGCCGCGTCCGACGACGACGATC
TGCCGCCGATGCAGATCCACGAAACGTCGACGTCGCTGCGCGATCATCTGATGGCGCAGTTGCGCGTCACGCAGGCGAGC
CCGCGCGATCGCGCGCTCGTCACGTTCCTGATCGAATCGCTCGACGACGACGGCTATCTGACCGCGTCGCTCGAGGAAGT
GCTCGCCGATCTGCCCGAGGAGCTCGAAGTCGACGTCGACGAACTGAATGCGGCGCTCGCGCTCGTGCACAGCTTCGATC
CCGCGGGCGTCGGCGCGCGCTCCGCATCCGAATGCCTGAAGCTGCAACTGCTGCGCCTGGATCCGTCCCCGACGCGCACG
CTCGCCCTCGATATCGTGTCGCAGCATCTCGAGCTGCTCGCCGCGCGCGATTTCACGCGCCTGCGCAAGCAACTGAAGGC
GACCGACGACGAGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTCGAGCCGTTCCCCGGCGCGGCATACGGCAAGGCCG
AAGCCGATTACGTGGTGCCCGACATCATCGTGAAGAAGACCGGCCAGAACTGGCAGGCGGAACTCAATCCGGAAGTGGTG
CCGAAGCTGCGGATCAACCATCTTTACGCGAACATCCTGCGCAACAGCCGGGGCGATCCGAGCACGGGATCGCTGAAGCA
GCAGCTTCAGGAAGCGCGCTGGCTGATCAAGAATATTCAGCAGCGATTCGAGACGATCCTGCGCGTCGCGCAGGCGATTG
TCGAACGTCAGAAGAATTTCTTTGCGCACGGCGAAATCGCGATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTG
GGCCTACACGAATCGACTGTCTCCCGTGTGACAACCGGCAAGTACATGCTGACCCCGTTCGGCACGCTTGAATTTAAGTA
CTTCTTCGGATCGCACGTATCGACAGACACCGGAGGCGCCGCGTCGTCGACAGCGATCCGCGCCCTCATCAAACAACTGA
TAGGAGCCGAAGACCCCAAATCCCCTCTTTCGGACAGTCGCATAGCCGAGCTGCTAGCGGAACAAGGGTTCGTGGTCGCG
CGCCGCACGGTTGCCAAATATCGCGAAGCCCTCAAGATCCCGGCGGTGAATCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(345-500)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(138-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.762

100

0.697