Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   MYA_RS12440 Genome accession   NC_017920
Coordinates   2759796..2761301 (+) Length   501 a.a.
NCBI ID   WP_041493891.1    Uniprot ID   -
Organism   Burkholderia sp. KJ006     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2754796..2766301
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MYA_RS12415 (MYA_2547) kdsD 2756037..2757020 (+) 984 WP_014723665.1 arabinose 5-phosphate isomerase KdsD -
  MYA_RS12420 (MYA_2548) - 2757020..2757556 (+) 537 WP_011886052.1 HAD family hydrolase -
  MYA_RS12425 (MYA_2549) lptC 2757560..2758174 (+) 615 WP_011886053.1 LPS export ABC transporter periplasmic protein LptC -
  MYA_RS12430 (MYA_2550) lptA 2758195..2758860 (+) 666 WP_011886054.1 lipopolysaccharide transport periplasmic protein LptA -
  MYA_RS12435 (MYA_2551) lptB 2758857..2759633 (+) 777 WP_014723666.1 LPS export ABC transporter ATP-binding protein -
  MYA_RS12440 (MYA_2552) rpoN/rpoN1 2759796..2761301 (+) 1506 WP_041493891.1 RNA polymerase factor sigma-54 Machinery gene
  MYA_RS12445 (MYA_2553) raiA 2761460..2761819 (+) 360 WP_011886057.1 ribosome-associated translation inhibitor RaiA -
  MYA_RS12450 (MYA_2554) - 2762014..2762526 (+) 513 WP_011886058.1 PTS sugar transporter subunit IIA -
  MYA_RS12455 (MYA_2555) hprK 2762656..2763624 (+) 969 WP_006483133.1 HPr(Ser) kinase/phosphatase -
  MYA_RS12460 (MYA_2556) rapZ 2763700..2764608 (+) 909 WP_014723670.1 RNase adapter RapZ -
  MYA_RS12465 (MYA_2557) - 2764636..2765274 (+) 639 WP_011886060.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54752.87 Da        Isoelectric Point: 4.8247

>NTDB_id=51293 MYA_RS12440 WP_041493891.1 2759796..2761301(+) (rpoN/rpoN1) [Burkholderia sp. KJ006]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLESDDEWIASPLRVAADGSLIAQTPPAPNGESGS
MNGSGQSSERAERDEPAATDEYDGYGSGDASDGPQWNLDEFGRSSGASDDDDLPPLQVQEAATSLREHLSAQLRVTQASP
RDRALVMFLIESLDDDGYLTASLDEVLADLPPELEVDGDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKQLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVMP
RLRINNLYANILRNSRGDPSTGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALRIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=51293 MYA_RS12440 WP_041493891.1 2759796..2761301(+) (rpoN/rpoN1) [Burkholderia sp. KJ006]
ATGAAAGCCAGCCTCCAACTCCGCCTGTCGCAACACCTTGCGTTGACGCCGCAGCTGCAGCAGTCGATTCGGCTTTTGCA
GCTGTCGACGCTCGAGCTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAATCCGCTGCTGGAAAGCGACGACGAATGGA
TCGCGAGCCCGCTGCGCGTCGCAGCCGACGGATCGCTGATCGCGCAGACGCCGCCCGCCCCGAACGGCGAGTCCGGCAGC
ATGAACGGCAGCGGCCAGTCGAGCGAGCGCGCCGAACGCGACGAGCCGGCCGCCACCGACGAATACGACGGCTATGGCTC
GGGCGACGCGAGCGACGGCCCGCAATGGAATCTCGACGAGTTCGGCCGCTCGAGCGGCGCGTCGGACGACGACGATCTCC
CGCCGCTGCAGGTCCAGGAAGCCGCCACATCGCTGCGCGAGCATCTGTCCGCGCAGCTGCGCGTCACGCAGGCCAGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTATCTGACCGCGTCGCTCGACGAAGTGCT
GGCCGACCTGCCGCCCGAACTGGAAGTCGACGGCGACGAGCTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGATCCGG
CCGGCGTCGGCGCGCGCTCGGCGTCCGAATGCCTGAAGCTGCAACTGCTGCGGCTCGATCCGTCCCCGACCCGCACGCTC
GCGCTGGAAATCGTCTCGCAGCATCTCGAGCTGCTCGCCGCGCGCGACTTCACGCGGCTGCGCAAGCAACTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCGGCCTACGGCAAGGCCGAGG
CCGACTACGTGGTACCCGACATCATCGTGAAGAAGGCCGGCCAGGGCTGGCTCGCGGAGCTCAATCCGGAGGTGATGCCG
CGCCTGCGGATCAACAACCTGTACGCGAACATTCTGCGCAACAGCCGCGGCGATCCGTCGACCGGATCGTTGAAGCAACA
GCTGCAGGAAGCACGCTGGCTGATCAAGAATATCCAGCAGCGATTCGACACGATCCTGCGTGTCGCGCAGGCTATTGTCG
AACGTCAGAAGAACTTCTTTGCGCACGGCGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGTAAGTACATGCTGACCCCGTTCGGGACGCTGGAGTTCAAGTACTT
CTTCGGATCGCACGTTTCGACCGACACCGGAGGCGCCGCATCGTCGACCGCCATCCGAGCCCTGATCAAGCAACTGATAG
GAGCTGAAGACCCAAAGTCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGATTCGTGGTCGCGCGC
CGCACGGTCGCGAAGTATCGCGAAGCCCTCAGGATCCCGGCAGTGAATCTGCGCAAATCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

67.836

100

0.695

Multiple sequence alignment