Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   I6G67_RS02290 Genome accession   NZ_CP065666
Coordinates   436554..437354 (+) Length   266 a.a.
NCBI ID   WP_004697674.1    Uniprot ID   -
Organism   Acinetobacter johnsonii strain FDAARGOS_910     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 431554..442354
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6G67_RS02260 (I6G67_02260) - 431793..432203 (+) 411 WP_004697663.1 hypothetical protein -
  I6G67_RS02265 (I6G67_02265) - 432322..433602 (+) 1281 WP_004697665.1 glycosyltransferase family 1 protein -
  I6G67_RS02270 (I6G67_02270) - 433795..434346 (+) 552 WP_004697666.1 phosphatase PAP2 family protein -
  I6G67_RS02275 (I6G67_02275) iscX 434437..434637 (+) 201 WP_004697668.1 Fe-S cluster assembly protein IscX -
  I6G67_RS02280 (I6G67_02280) ndk 434748..435179 (+) 432 WP_004697669.1 nucleoside-diphosphate kinase -
  I6G67_RS02285 (I6G67_02285) rlmN 435302..436537 (+) 1236 WP_004697671.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  I6G67_RS02290 (I6G67_02290) pilF 436554..437354 (+) 801 WP_004697674.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  I6G67_RS02295 (I6G67_02295) - 437345..438121 (+) 777 WP_004697676.1 helix-turn-helix domain-containing protein -
  I6G67_RS02300 (I6G67_02300) ispG 438140..439255 (+) 1116 WP_004697677.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  I6G67_RS02305 (I6G67_02305) hisS 439252..440547 (+) 1296 WP_004697679.1 histidine--tRNA ligase -
  I6G67_RS02310 (I6G67_02310) - 440575..441273 (+) 699 WP_004697681.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30076.05 Da        Isoelectric Point: 9.1263

>NTDB_id=512780 I6G67_RS02290 WP_004697674.1 436554..437354(+) (pilF) [Acinetobacter johnsonii strain FDAARGOS_910]
MKKPVSKLYLALTLGVSVWYLVGCQTVNTKGDPSKAVQVRTQLAAEYIKSNDYDAAKRTLDQALDIDSRDASANMMMGVL
LQREGSPQNVEKAERYFKHAIAAEPKNAQARNNYGTYLYQIGRYNDAIDQLQVAGSTLGYDQRYRALENLGRAYLQVGRV
AEAEAAFKQALQVNSGAYLAMIEMAEISYLRQQNAEATQYYEQFVRAVGEKNLDARALWIGIRIARANHDTMGSQVLVNQ
LRALYPDSQEYKRYLQLQYTTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=512780 I6G67_RS02290 WP_004697674.1 436554..437354(+) (pilF) [Acinetobacter johnsonii strain FDAARGOS_910]
TTGAAAAAACCCGTTTCTAAACTTTACCTTGCGCTGACACTGGGTGTGTCAGTGTGGTACTTGGTGGGTTGCCAAACCGT
GAATACGAAAGGTGACCCAAGTAAAGCAGTACAAGTTAGAACGCAATTGGCGGCTGAATATATTAAGTCGAATGATTACG
ATGCTGCGAAACGCACATTGGATCAAGCATTAGACATCGACTCTCGTGATGCATCTGCAAATATGATGATGGGCGTTTTG
TTACAGCGTGAAGGCAGTCCACAAAATGTGGAAAAGGCAGAGCGTTATTTTAAGCATGCAATTGCGGCTGAGCCAAAAAA
TGCACAGGCTCGTAACAACTACGGTACGTATTTATATCAAATTGGGCGTTATAATGACGCGATTGATCAACTTCAAGTTG
CAGGTTCAACTTTAGGGTATGATCAACGTTATCGAGCATTGGAGAATTTAGGTCGTGCGTATCTACAAGTAGGTCGAGTG
GCGGAAGCAGAAGCTGCTTTTAAACAAGCTCTACAGGTAAACTCAGGTGCCTATCTAGCCATGATCGAAATGGCGGAAAT
TTCATATTTACGTCAGCAAAATGCTGAAGCTACGCAGTACTATGAACAGTTTGTGCGAGCGGTTGGCGAGAAAAACTTAG
ATGCACGTGCGCTTTGGATTGGGATCCGAATTGCGCGAGCAAATCATGACACGATGGGTTCGCAAGTACTGGTCAACCAG
TTACGTGCATTGTATCCAGACAGTCAAGAATACAAACGTTATTTGCAATTACAGTACACTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_19)

(89-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

68.657

100

0.692
  pilF Acinetobacter baylyi ADP1

64.662

100

0.647