Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KTJ86_RS16685 Genome accession   NZ_CP077969
Coordinates   3379762..3380439 (+) Length   225 a.a.
NCBI ID   WP_000178154.1    Uniprot ID   P25960
Organism   Escherichia coli strain JM83     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3374762..3385439
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KTJ86_RS16650 gspG 3375242..3375679 (+) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -
  KTJ86_RS16655 gspH 3375687..3376196 (+) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  KTJ86_RS16660 gspI 3376193..3376570 (+) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  KTJ86_RS16665 gspJ 3376563..3377150 (+) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  KTJ86_RS16670 gspK 3377143..3378126 (+) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  KTJ86_RS16675 gspL 3378141..3379304 (+) 1164 WP_001115238.1 type II secretion system protein GspL -
  KTJ86_RS16680 gspM 3379301..3379762 (+) 462 WP_001295161.1 type II secretion system protein GspM -
  KTJ86_RS16685 pilD 3379762..3380439 (+) 678 WP_000178154.1 prepilin peptidase GspO Machinery gene
  KTJ86_RS16690 bfr 3380468..3380944 (-) 477 WP_000675504.1 bacterioferritin -
  KTJ86_RS16695 bfd 3381016..3381210 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  KTJ86_RS16700 chiA 3381379..3384072 (-) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24956.82 Da        Isoelectric Point: 7.6353

>NTDB_id=512085 KTJ86_RS16685 WP_000178154.1 3379762..3380439(+) (pilD) [Escherichia coli strain JM83]
MTMLLPLFILVGFIADYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=512085 KTJ86_RS16685 WP_000178154.1 3379762..3380439(+) (pilD) [Escherichia coli strain JM83]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P25960

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378