Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   I5367_RS17480 Genome accession   NZ_CP065539
Coordinates   3562999..3564144 (+) Length   381 a.a.
NCBI ID   WP_012118686.1    Uniprot ID   A3KLD4
Organism   Bacillus amyloliquefaciens strain DGL1     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 3557999..3569144
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I5367_RS17460 (I5367_17460) - 3558897..3559325 (-) 429 WP_007407634.1 DUF1934 domain-containing protein -
  I5367_RS17465 (I5367_17465) - 3559479..3559990 (+) 512 Protein_3401 bacteriocin biosynthesis protein AlbG -
  I5367_RS17470 (I5367_17470) - 3559997..3561373 (-) 1377 WP_012118684.1 YncE family protein -
  I5367_RS17475 (I5367_17475) - 3561413..3562768 (-) 1356 WP_012118685.1 YncE family protein -
  I5367_RS17480 (I5367_17480) rapF 3562999..3564144 (+) 1146 WP_012118686.1 tetratricopeptide repeat protein Regulator
  I5367_RS17485 (I5367_17485) phrF 3564128..3564247 (+) 120 WP_007407639.1 PhrC/PhrF family phosphatase-inhibitory pheromone Regulator
  I5367_RS17490 (I5367_17490) - 3564445..3564648 (+) 204 WP_007407640.1 helix-turn-helix transcriptional regulator -
  I5367_RS17495 (I5367_17495) - 3564662..3564865 (+) 204 WP_007407641.1 hypothetical protein -
  I5367_RS17500 (I5367_17500) - 3564900..3565085 (+) 186 WP_007407642.1 DUF4177 domain-containing protein -
  I5367_RS17505 (I5367_17505) - 3565271..3565552 (+) 282 WP_007407643.1 hypothetical protein -
  I5367_RS17510 (I5367_17510) - 3565596..3566108 (+) 513 WP_023357177.1 stage II sporulation protein M -
  I5367_RS17515 (I5367_17515) - 3566699..3566917 (-) 219 WP_003151056.1 helix-turn-helix transcriptional regulator -
  I5367_RS17520 (I5367_17520) speB 3567085..3567957 (-) 873 WP_007614448.1 agmatinase -
  I5367_RS17525 (I5367_17525) speE 3568016..3568846 (-) 831 WP_003151052.1 spermidine synthase -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45500.54 Da        Isoelectric Point: 4.5407

>NTDB_id=511796 I5367_RS17480 WP_012118686.1 3562999..3564144(+) (rapF) [Bacillus amyloliquefaciens strain DGL1]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEDDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQALLTGRLDYYFNFFRGMYELECREYLSAIQFFKKAEYKLDFVKDCIEKAEFYFKMSESYYYMKQTYFS
MDYARQAYKIYHKQEAYNIRVLQCHSLFATNFLDLKQYDEAIQHFKKAYAMAEAEQQPQLMGRTLYNIGLCFNSQENYKP
AIDYIKRAIAVFEDGNIITSLPQAYFLITQIHYKIGNMAIARQYHDKGVSYAEEAEDSLYIAEYEFLESLYVGEPDEEAI
MECFDFLRDKSMYADLEDFALDVAKYYHERENFEKASAYFLKVEETRQQIQGGVKLYEIEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=511796 I5367_RS17480 WP_012118686.1 3562999..3564144(+) (rapF) [Bacillus amyloliquefaciens strain DGL1]
GTGGCGAGTGTTTTATCATCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAACTTGCAGGTCTGGAAGATGATCAGGATCTTCATCTATACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCTAGA
CTATCGGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCTGCTGACCGGACGTCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGAATGCCGTGAATATCTTTCAGCGATTCAGTTTTTTAAGAAAGCTGAATATAAGCTTGATTTTG
TCAAAGATTGTATCGAGAAAGCTGAATTTTATTTTAAGATGTCAGAGTCCTACTATTATATGAAACAGACTTATTTTTCT
ATGGATTATGCCCGGCAGGCTTATAAAATTTATCATAAGCAGGAGGCATATAATATAAGGGTGCTTCAGTGCCATTCATT
GTTCGCTACCAACTTTCTTGACTTAAAGCAATATGATGAAGCAATTCAGCATTTTAAAAAGGCTTATGCCATGGCAGAGG
CAGAACAGCAGCCACAATTAATGGGCCGGACTCTATATAATATCGGACTTTGTTTTAACAGCCAAGAAAATTACAAACCA
GCCATTGATTACATAAAACGGGCAATAGCTGTTTTTGAAGATGGGAACATCATCACTTCTCTCCCGCAAGCCTATTTCTT
AATCACACAAATACATTATAAAATCGGAAATATGGCCATAGCCAGACAATATCATGACAAAGGGGTATCTTATGCAGAGG
AGGCAGAAGATTCCCTATATATCGCCGAGTATGAATTTTTAGAATCTTTATATGTCGGTGAGCCTGATGAAGAAGCAATC
ATGGAATGTTTTGACTTTCTCAGAGATAAATCGATGTATGCTGATCTTGAAGATTTTGCCTTAGATGTGGCAAAATATTA
TCATGAAAGAGAAAATTTCGAGAAGGCTTCCGCATATTTTTTAAAGGTGGAAGAAACAAGACAGCAGATTCAAGGAGGTG
TGAAGTTGTATGAAATTGAAGTATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_12)

(189-253)

response regulator aspartate phosphatase H, N terminal (RapH_N)

(5-65)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A3KLD4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

78.215

100

0.782
  rapC Bacillus subtilis subsp. subtilis str. 168

56.579

99.738

0.564