Detailed information NTG0511595

Bioinformatically predicted

Overview

• Name: radA
• Type: Machinery gene
• Locus tag: I6U31_RS09200
• Genome accession: NZ_CP065522
• Coordinates: 1774797..1776170 (-)
• Length: 457 a.a.
• NCBI ID: WP_004562734.1
• Uniprot ID: A0A806TIP1
• Organism: Limosilactobacillus fermentum strain YLF016
• Function: homologous recombination (predicted from homology) Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Prophage	1774797..1783769	1774797..1776170	within	0

Gene organization within MGE regions

Location: 1774797..1783769

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6U31_RS09200 (I6U31_09200)	radA	1774797..1776170 (-)	1374	WP_004562734.1	DNA repair protein RadA	Machinery gene
I6U31_RS09205 (I6U31_09205)	-	1776183..1776722 (-)	540	WP_216284863.1	dUTP diphosphatase	-
I6U31_RS09210 (I6U31_09210)	-	1776922..1777233 (+)	312	WP_004562732.1	GNAT family N-acetyltransferase	-
I6U31_RS09215 (I6U31_09215)	rpiA	1777245..1777931 (+)	687	WP_187350369.1	ribose-5-phosphate isomerase RpiA	-
I6U31_RS09220 (I6U31_09220)	-	1777959..1778573 (+)	615	WP_004563340.1	sugar O-acetyltransferase	-
I6U31_RS09225 (I6U31_09225)	-	1778637..1779974 (+)	1338	WP_070447627.1	aminopeptidase C	-
I6U31_RS09230 (I6U31_09230)	-	1780020..1781306 (-)	1287	WP_216284864.1	ISL3 family transposase	-
I6U31_RS09235 (I6U31_09235)	-	1781397..1782395 (-)	999	WP_014562466.1	IS30 family transposase	-
I6U31_RS09240 (I6U31_09240)	-	1782894..1783769 (-)	876	WP_004562728.1	alpha/beta hydrolase	-

Sequence

Protein

• Length: 457 a.a.
• Molecular weight: 49381.79 Da
• Isoelectric Point: 8.7358

>NTDB_id=511595 I6U31_RS09200 WP_004562734.1 1774797..1776170(-) (radA) [Limosilactobacillus fermentum strain YLF016]
MAKAKTHFVCQNCGYNSPRYLGRCPNCGQWSTLVEEVEQASTPAAKNATATLTGIVARPQRIQEIDTKKTPRVKTRLNEL
NRVLGGGIVPGSLVLIGGDPGIGKSTLLLQVSGQLSVEHHKVLYVSGEESASQIKLRAQRLDVSGEDFYIYPETNMEAIR
ATIDSIQPEFVIIDSVQTMQAADVTSAIGSVSQIRAVTAQLMQIAKGQNITVFVVGHVTKGGVLAGPKILEHMVDTVLYF
EGDLHHTYRILRSVKNRFGSTNELGIFEMNTRGLTEVANPSEIFLEERLKDATGSAVVVSLEGTRPILVEIQALITPTVF
GNAQRTATGLNRNRVSLIMAVLEKRANLLLQNQDAYLKAAGGVKLDEPAIDLAIAVAIASSYRDKGTRPTDAFVGEVGLT
GEIRRVSRIEQRVAEAAKLGFKRILVPKNNLAGWNPPQDIEVVGVTTLGEALKIALG

Nucleotide

• Length: 1374 bp

>NTDB_id=511595 I6U31_RS09200 WP_004562734.1 1774797..1776170(-) (radA) [Limosilactobacillus fermentum strain YLF016]
ATGGCAAAAGCCAAAACACACTTTGTCTGCCAAAACTGCGGTTACAATTCACCGCGCTATTTAGGGCGGTGTCCCAATTG
TGGCCAGTGGAGTACCTTAGTTGAAGAAGTCGAGCAGGCAAGTACCCCGGCGGCTAAAAACGCTACGGCAACCTTAACCG
GGATTGTGGCCCGGCCCCAGCGAATCCAAGAAATTGACACCAAAAAAACGCCCCGGGTTAAGACCCGCTTAAATGAGTTA
AACCGGGTGCTAGGTGGCGGAATTGTACCCGGCTCGTTGGTCTTAATTGGTGGGGATCCCGGGATCGGAAAGTCCACCCT
GCTCTTACAAGTGTCGGGGCAACTGAGCGTGGAACACCACAAGGTTCTTTACGTTTCCGGGGAAGAATCGGCCTCCCAAA
TTAAATTGCGGGCCCAACGCTTAGACGTTTCCGGCGAGGACTTTTATATCTACCCGGAAACCAACATGGAAGCCATCCGG
GCGACGATTGATTCCATTCAGCCCGAGTTTGTAATCATTGACTCGGTTCAGACGATGCAGGCGGCCGACGTGACCTCGGC
GATTGGGTCGGTCTCCCAAATCCGGGCGGTCACCGCCCAGTTGATGCAGATTGCCAAGGGGCAAAACATCACGGTCTTCG
TGGTTGGTCACGTCACCAAGGGTGGGGTCTTAGCCGGGCCCAAGATCTTGGAACACATGGTTGACACGGTGCTGTACTTT
GAAGGGGACCTCCACCACACCTACCGGATTTTACGGTCGGTCAAAAACCGGTTTGGGTCCACCAATGAGTTAGGGATCTT
CGAAATGAATACCCGCGGTTTGACCGAGGTCGCAAACCCCTCGGAAATCTTTTTGGAAGAGCGGCTTAAGGATGCCACCG
GGTCGGCCGTGGTCGTATCCCTGGAGGGAACCCGACCGATCCTGGTTGAAATCCAGGCCTTGATCACGCCAACCGTCTTT
GGAAACGCCCAAAGAACGGCGACAGGTTTAAACCGCAACCGGGTTTCCTTGATCATGGCGGTCCTAGAAAAACGGGCGAA
CCTGCTCTTACAAAACCAGGATGCCTACTTGAAGGCGGCCGGGGGCGTTAAGTTAGACGAGCCGGCCATCGACCTTGCGA
TCGCGGTGGCAATTGCTTCTTCTTACCGGGATAAGGGGACCCGGCCGACCGACGCCTTCGTCGGTGAAGTTGGCTTAACC
GGGGAGATCCGTCGGGTGAGCCGAATTGAACAGCGGGTGGCCGAAGCCGCTAAACTAGGCTTTAAACGGATTTTAGTGCC
CAAAAACAATTTAGCGGGTTGGAACCCGCCGCAAGACATCGAAGTCGTGGGGGTAACGACCCTTGGCGAAGCACTTAAAA
TTGCCCTTGGATAG

Domains

Predicted by InterproScan.

• Rubredoxin metal binding domain (Zn_ribbon_LapB) (8-35)

• Subunit ChlI of Mg-chelatase (ChlI) (364-430)

• AAA domain (AAA_25) (64-222)

3D structure

• AlphaFold DB: A0A806TIP1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
radA	Streptococcus mitis SK321	68.212	99.125	0.676
radA	Streptococcus mitis NCTC 12261	67.991	99.125	0.674
radA	Streptococcus pneumoniae Rx1	67.991	99.125	0.674
radA	Streptococcus pneumoniae D39	67.991	99.125	0.674
radA	Streptococcus pneumoniae R6	67.991	99.125	0.674
radA	Streptococcus pneumoniae TIGR4	67.991	99.125	0.674
radA	Bacillus subtilis subsp. subtilis str. 168	63.676	100	0.637