Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   HR078_RS08245 Genome accession   NZ_CP065513
Coordinates   1666043..1667419 (-) Length   458 a.a.
NCBI ID   WP_013440031.1    Uniprot ID   A0A9Q4F5U1
Organism   Lactobacillus delbrueckii subsp. lactis strain CIDCA 133     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1661043..1672419
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HR078_RS08225 (HR078_08235) - 1663246..1663455 (-) 210 WP_003612511.1 hypothetical protein -
  HR078_RS08230 (HR078_08240) - 1663619..1664560 (-) 942 WP_231523494.1 EAL domain-containing protein -
  HR078_RS08235 (HR078_08245) - 1664488..1664838 (-) 351 WP_231523493.1 EAL domain-containing protein -
  HR078_RS08240 (HR078_08250) - 1664985..1665932 (-) 948 WP_003612526.1 glycosyltransferase family 8 protein -
  HR078_RS08245 (HR078_08255) radA 1666043..1667419 (-) 1377 WP_013440031.1 DNA repair protein RadA Machinery gene
  HR078_RS08250 (HR078_08260) - 1667419..1667973 (-) 555 WP_013440032.1 dUTP diphosphatase -
  HR078_RS08255 (HR078_08265) - 1668078..1668365 (+) 288 WP_013440033.1 hypothetical protein -
  HR078_RS08260 (HR078_08270) pepC 1668455..1669804 (+) 1350 WP_013440034.1 aminopeptidase C -
  HR078_RS08265 (HR078_08275) - 1669871..1670164 (-) 294 WP_003615860.1 hypothetical protein -
  HR078_RS08270 (HR078_08280) - 1670158..1671093 (-) 936 WP_003612517.1 AAA family ATPase -
  HR078_RS08275 (HR078_08285) fabZ 1671205..1671666 (-) 462 WP_002879757.1 3-hydroxyacyl-ACP dehydratase FabZ -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49965.45 Da        Isoelectric Point: 6.8358

>NTDB_id=511526 HR078_RS08245 WP_013440031.1 1666043..1667419(-) (radA) [Lactobacillus delbrueckii subsp. lactis strain CIDCA 133]
MARARTQYKCRSCGYISASYLGRCPNCGAWNQFEKESQEVRQTSTKATPSRLMASVGAKEAVKMSTVKAEKEGRVLTKSS
ELNRVLGGGIVPGSLVLIGGDPGIGKSTLMLQIMSELAEKYKVLYVSGEESASQIKLRADRLGLGQSPMLLYPESNMEHI
RDQIDDVKPDFVVIDSIQTMNEPSLDSMVGSASQVREVTAELMKIAKLDQVTVFVIGHVTKEGSIAGPKILEHMVDTVLY
FEGDEHHAYRILHSVKNRFGAANEIGMFEMKTEGLAEVTNPSAVFLDERLPDSTGSAVVVSLEGTRPILGEIQALVTPTA
FGYAKRTTSGLDYNRVSLLLAVLEKRGNLMLQNQDAYLSATGGIRLNEPAIDLAIAMAIASSYYDKEISATDCFVGEIGL
TGEVRRVNQIQARVKEAGKVGFKRIFIPKYNMSPALQDLGIEVIPVSSVRQALHYLFS

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=511526 HR078_RS08245 WP_013440031.1 1666043..1667419(-) (radA) [Lactobacillus delbrueckii subsp. lactis strain CIDCA 133]
ATGGCCAGAGCTAGAACCCAGTACAAGTGCCGGTCTTGCGGCTACATCTCCGCTTCTTACCTGGGCCGGTGCCCGAACTG
CGGGGCCTGGAACCAGTTTGAAAAAGAAAGCCAGGAGGTCCGCCAGACTTCTACTAAAGCTACCCCCAGCCGCCTGATGG
CCAGCGTGGGTGCTAAAGAAGCCGTTAAAATGTCTACCGTCAAGGCGGAAAAAGAAGGGCGGGTCTTAACCAAGTCCTCT
GAACTTAACCGGGTCTTAGGCGGCGGGATCGTACCAGGCTCTTTGGTCTTGATTGGCGGGGACCCCGGCATCGGCAAGTC
CACTTTGATGCTGCAGATCATGAGCGAACTGGCCGAAAAATACAAGGTCCTCTACGTCTCCGGGGAAGAATCTGCCAGCC
AGATCAAGCTGCGGGCTGACCGGCTCGGCCTGGGCCAAAGCCCTATGCTTTTATACCCGGAAAGCAACATGGAACATATC
CGCGACCAGATCGACGACGTTAAGCCAGATTTTGTCGTGATCGACTCCATCCAGACCATGAACGAACCCAGCCTGGACTC
CATGGTGGGCTCAGCTTCCCAGGTCCGGGAAGTTACCGCGGAACTGATGAAGATCGCCAAGCTGGACCAAGTCACTGTCT
TTGTCATTGGCCACGTGACCAAGGAAGGGTCCATCGCCGGGCCGAAAATCCTGGAGCACATGGTTGACACCGTCCTTTAC
TTTGAAGGTGACGAGCACCATGCCTACCGGATCCTGCACTCAGTCAAGAACCGTTTTGGGGCGGCCAACGAAATCGGCAT
GTTTGAAATGAAGACCGAAGGCCTGGCAGAAGTGACCAATCCTTCCGCTGTCTTCTTAGATGAACGCTTGCCCGACTCAA
CTGGGTCAGCCGTGGTCGTTTCTTTAGAAGGCACCCGGCCGATCTTGGGCGAAATCCAGGCCCTGGTCACCCCAACCGCC
TTCGGCTATGCCAAGCGGACTACTTCGGGCCTGGACTACAACCGGGTCTCCCTGCTTTTAGCCGTTTTGGAAAAAAGGGG
GAACCTAATGCTGCAGAACCAGGACGCCTATTTATCAGCGACCGGCGGCATCCGCTTAAACGAACCGGCCATTGACCTGG
CCATCGCCATGGCCATTGCCTCCAGTTACTATGACAAGGAAATCTCCGCGACTGACTGCTTTGTCGGGGAAATCGGCCTG
ACCGGGGAAGTCCGCCGGGTCAACCAGATCCAGGCCCGAGTCAAGGAAGCCGGCAAGGTTGGCTTCAAGCGGATTTTTAT
CCCGAAATACAATATGAGCCCGGCCTTGCAGGATTTGGGGATTGAGGTAATCCCGGTCTCCAGCGTCCGCCAGGCCCTCC
ACTACCTTTTCTCATAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(364-431)

AAA domain (AAA_25)

(70-223)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

56.332

100

0.563
  radA Streptococcus mitis NCTC 12261

56.332

100

0.563
  radA Streptococcus pneumoniae Rx1

56.332

100

0.563
  radA Streptococcus pneumoniae D39

56.332

100

0.563
  radA Streptococcus pneumoniae R6

56.332

100

0.563
  radA Streptococcus pneumoniae TIGR4

56.332

100

0.563
  radA Bacillus subtilis subsp. subtilis str. 168

55.604

99.345

0.552