Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   IQ781_RS23105 Genome accession   NZ_CP065509
Coordinates   4512085..4512519 (+) Length   144 a.a.
NCBI ID   WP_000811494.1    Uniprot ID   A0A9Q6M5X1
Organism   Bacillus sp. N447-1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 4507085..4517519
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IQ781_RS23085 (IQ781_23025) - 4507999..4509162 (-) 1164 WP_000434582.1 aminotransferase A -
  IQ781_RS23090 (IQ781_23030) - 4509285..4509485 (-) 201 WP_000929278.1 hypothetical protein -
  IQ781_RS23095 (IQ781_23035) kinB 4509796..4511070 (+) 1275 WP_000420370.1 sporulation sensor histidine kinase KinB -
  IQ781_RS23100 (IQ781_23040) - 4511109..4511858 (-) 750 WP_000388471.1 DUF3967 domain-containing protein -
  IQ781_RS23105 (IQ781_23045) nucA/comI 4512085..4512519 (+) 435 WP_000811494.1 DNA-entry nuclease Machinery gene
  IQ781_RS23110 (IQ781_23050) comJ 4512556..4512945 (+) 390 WP_048655569.1 competence protein ComJ -
  IQ781_RS23115 (IQ781_23055) metE 4512984..4515272 (-) 2289 WP_139846721.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  IQ781_RS23120 (IQ781_23060) - 4515786..4516343 (-) 558 WP_139846722.1 PadR family transcriptional regulator -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16054.43 Da        Isoelectric Point: 8.4331

>NTDB_id=511454 IQ781_RS23105 WP_000811494.1 4512085..4512519(+) (nucA/comI) [Bacillus sp. N447-1]
MKQLKGIIISIIAILSILVAVYEVLVPEETSVKKTNTYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=511454 IQ781_RS23105 WP_000811494.1 4512085..4512519(+) (nucA/comI) [Bacillus sp. N447-1]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATCGCAATTCTGTCTATTTTAGTAGCAGTTTATGAAGTACTTGTGCC
AGAGGAAACAAGTGTAAAGAAAACGAATACGTATGATCAAGTGTTAGAATTTCCGAAAGAACGTTATCCAGAGACAGGAA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGATCGTGGTGGTGCTGCAGATAGAAGGAAA
TTGTCGTTAGCACCATATCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGCAAGGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCGGCGGATAACCGCGGAGCAGGTTCTTGGGTAGGGAATAAGTTAGATAAATACC
CAGATGGTACGCGAGTAAAATTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.912

78.472

0.549