Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   ST90729_RS08850 Genome accession   NZ_CP065480
Coordinates   1711974..1712915 (-) Length   313 a.a.
NCBI ID   WP_022096896.1    Uniprot ID   -
Organism   Streptococcus thermophilus strain 90729     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1706974..1717915
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ST90729_RS08810 (ST90729_08775) - 1707050..1708243 (-) 1194 WP_014608740.1 acetate kinase -
  ST90729_RS08815 (ST90729_08780) comYH 1708299..1709255 (-) 957 WP_011226622.1 class I SAM-dependent methyltransferase Machinery gene
  ST90729_RS08820 (ST90729_08785) comGG 1709300..1709617 (-) 318 WP_141326604.1 competence type IV pilus minor pilin ComGG -
  ST90729_RS08825 (ST90729_08790) comYF 1709595..1710032 (-) 438 WP_014608742.1 competence type IV pilus minor pilin ComGF Machinery gene
  ST90729_RS08830 (ST90729_08795) comGE 1710016..1710309 (-) 294 WP_011226625.1 competence type IV pilus minor pilin ComGE -
  ST90729_RS08835 (ST90729_08800) comYD 1710281..1710709 (-) 429 WP_011226626.1 competence type IV pilus minor pilin ComGD Machinery gene
  ST90729_RS08840 (ST90729_08805) comYC 1710669..1710995 (-) 327 WP_002946126.1 competence type IV pilus major pilin ComGC Machinery gene
  ST90729_RS08845 (ST90729_08810) comYB 1710992..1712092 (-) 1101 WP_271307389.1 competence type IV pilus assembly protein ComGB Machinery gene
  ST90729_RS08850 (ST90729_08815) comGA/cglA/cilD 1711974..1712915 (-) 942 WP_022096896.1 competence type IV pilus ATPase ComGA Machinery gene
  ST90729_RS08855 (ST90729_08820) - 1712996..1713358 (-) 363 WP_011226630.1 DUF1033 family protein -
  ST90729_RS08860 (ST90729_08825) rpoC 1713517..1717155 (-) 3639 WP_011226631.1 DNA-directed RNA polymerase subunit beta' -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35409.56 Da        Isoelectric Point: 6.3927

>NTDB_id=510217 ST90729_RS08850 WP_022096896.1 1711974..1712915(-) (comGA/cglA/cilD) [Streptococcus thermophilus strain 90729]
MVTEFAKEMIKNADSCGAQDIYVIPRQDNYELYMRVGQERRLIDVYRPDFMASLIGHFKFVAGMMVGEKRRSQLGSCDYD
CGDGHLVSLRLSTVGDYRGLESLVIRILHSERRELVYWNQGIQPIKDALDYRGLYLFAGPVGSGKTTLMHELVQERFKGQ
QVISIEDPVEIKQDNVLQLQVNQAIDMTYDSLIKLSLRHRPDVLIIGEIRDKETARAVIRASLTGVTVLSTIHAKSIAGV
YERLLDLGVDKSELDNALQGIAYMRLIKGGGVIDFARENFQNHSSTNWNQQLEGLVKQGCLTERDIQGEKIKD

Nucleotide


Download         Length: 942 bp        

>NTDB_id=510217 ST90729_RS08850 WP_022096896.1 1711974..1712915(-) (comGA/cglA/cilD) [Streptococcus thermophilus strain 90729]
ATGGTAACAGAATTTGCTAAGGAAATGATAAAAAATGCTGATAGTTGTGGGGCTCAAGACATCTATGTCATTCCACGTCA
GGATAATTATGAGCTCTATATGCGAGTCGGGCAAGAGAGGAGATTGATTGATGTATATCGGCCTGATTTCATGGCTAGTC
TTATTGGTCACTTTAAATTTGTGGCGGGAATGATGGTGGGTGAGAAGCGTAGGAGTCAACTGGGTTCATGTGACTATGAT
TGTGGTGACGGTCACTTGGTTTCTCTGCGTTTATCAACTGTTGGAGACTATCGAGGTTTGGAGAGCTTGGTTATCCGTAT
CCTACATTCTGAACGTCGGGAATTAGTGTATTGGAATCAAGGAATCCAGCCTATTAAGGATGCCTTGGATTATAGAGGAC
TGTATCTCTTTGCGGGTCCCGTAGGTTCTGGGAAGACCACACTTATGCATGAGTTGGTTCAGGAGCGTTTTAAGGGGCAG
CAGGTAATTTCGATTGAGGATCCTGTTGAAATCAAACAGGATAATGTCTTGCAACTCCAGGTTAATCAGGCGATTGATAT
GACCTATGATAGTTTGATTAAGCTATCTCTACGTCACCGTCCAGATGTTTTGATTATTGGGGAGATTCGGGATAAGGAGA
CTGCCCGGGCAGTTATTAGAGCTAGTCTAACAGGTGTAACTGTTCTTTCAACTATTCATGCTAAGAGTATTGCTGGCGTT
TACGAACGTCTCTTGGACCTTGGCGTAGATAAGTCTGAGTTGGATAATGCTCTACAAGGCATTGCCTACATGCGTTTGAT
CAAGGGAGGAGGTGTGATTGATTTTGCCAGAGAAAATTTCCAAAACCATTCGTCGACCAACTGGAATCAGCAGTTGGAAG
GTTTGGTTAAACAAGGATGTCTCACTGAGAGGGATATCCAAGGGGAAAAAATTAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

65.595

99.361

0.652
  comYA Streptococcus mutans UA159

64.856

100

0.649
  comYA Streptococcus mutans UA140

64.856

100

0.649
  comGA/cglA/cilD Streptococcus pneumoniae D39

64.309

99.361

0.639
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

64.309

99.361

0.639
  comGA/cglA/cilD Streptococcus pneumoniae R6

64.309

99.361

0.639
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

64.309

99.361

0.639
  comYA Streptococcus gordonii str. Challis substr. CH1

63.259

100

0.633
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

61.538

99.681

0.613
  comGA Lactococcus lactis subsp. cremoris KW2

51.125

99.361

0.508
  comGA Latilactobacillus sakei subsp. sakei 23K

38.816

97.125

0.377