Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiF   Type   Regulator
Locus tag   ST4134_RS06825 Genome accession   NZ_CP065478
Coordinates   1314319..1315248 (-) Length   309 a.a.
NCBI ID   WP_002951426.1    Uniprot ID   A0A7U7CIU5
Organism   Streptococcus thermophilus strain 4134     
Function   internalize XIP (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 1298788..1369821 1314319..1315248 within 0


Gene organization within MGE regions


Location: 1298788..1369821
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ST4134_RS06750 (ST4134_06735) liaF 1298796..1299494 (-) 699 WP_011226289.1 cell wall-active antibiotics response protein LiaF -
  ST4134_RS06755 - 1299749..1299916 (-) 168 WP_014608530.1 potassium channel family protein -
  ST4134_RS06760 (ST4134_06745) stkP/pknB 1300554..1302425 (-) 1872 WP_024703938.1 Stk1 family PASTA domain-containing Ser/Thr kinase Regulator
  ST4134_RS06765 (ST4134_06750) - 1302425..1303162 (-) 738 WP_002946881.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  ST4134_RS06770 (ST4134_06755) rsmB 1303206..1304528 (-) 1323 WP_014608533.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  ST4134_RS06775 (ST4134_06760) fmt 1304518..1305453 (-) 936 WP_014608534.1 methionyl-tRNA formyltransferase -
  ST4134_RS06780 (ST4134_06765) - 1305471..1307867 (-) 2397 WP_024703939.1 primosomal protein N' -
  ST4134_RS06785 (ST4134_06770) rpoZ 1308009..1308323 (-) 315 WP_002951415.1 DNA-directed RNA polymerase subunit omega -
  ST4134_RS06790 (ST4134_06775) gmk 1308345..1308974 (-) 630 WP_014608537.1 guanylate kinase -
  ST4134_RS06795 (ST4134_06780) ftsY 1309200..1310591 (-) 1392 WP_014608538.1 signal recognition particle-docking protein FtsY -
  ST4134_RS06800 (ST4134_06785) - 1310605..1311420 (-) 816 WP_011226298.1 Cof-type HAD-IIB family hydrolase -
  ST4134_RS06805 (ST4134_06790) - 1311413..1312207 (-) 795 WP_011681412.1 HAD-IIB family hydrolase -
  ST4134_RS06810 (ST4134_06795) - 1312392..1312769 (+) 378 WP_011681413.1 GntR family transcriptional regulator -
  ST4134_RS06815 (ST4134_06800) - 1312774..1313472 (+) 699 WP_023909821.1 ABC transporter ATP-binding protein -
  ST4134_RS06820 (ST4134_06805) - 1313484..1314269 (+) 786 WP_002951425.1 hypothetical protein -
  ST4134_RS06825 (ST4134_06810) amiF 1314319..1315248 (-) 930 WP_002951426.1 ATP-binding cassette domain-containing protein Regulator
  ST4134_RS06830 (ST4134_06815) amiE 1315241..1316326 (-) 1086 WP_011226304.1 ABC transporter ATP-binding protein Regulator
  ST4134_RS06835 (ST4134_06820) amiD 1316336..1317262 (-) 927 WP_002946409.1 oligopeptide ABC transporter permease OppC Regulator
  ST4134_RS06840 (ST4134_06825) amiC 1317262..1318755 (-) 1494 WP_011681416.1 ABC transporter permease Regulator
  ST4134_RS06845 (ST4134_06830) amiA 1318817..1320784 (-) 1968 WP_024703940.1 peptide ABC transporter substrate-binding protein Regulator
  ST4134_RS06850 (ST4134_06835) - 1321140..1322317 (+) 1178 Protein_1295 ISL3 family transposase -
  ST4134_RS06855 (ST4134_06840) amiA3 1322362..1324335 (-) 1974 WP_011681419.1 peptide ABC transporter substrate-binding protein Regulator
  ST4134_RS06860 (ST4134_06845) - 1324640..1325787 (+) 1148 WP_095559415.1 IS3 family transposase -
  ST4134_RS06865 (ST4134_06850) - 1325825..1326009 (-) 185 Protein_1298 IS3 family transposase -
  ST4134_RS06870 - 1326003..1326413 (-) 411 Protein_1299 ATP-binding cassette domain-containing protein -
  ST4134_RS06875 (ST4134_06865) - 1326445..1326759 (-) 315 Protein_1300 TatD family hydrolase -
  ST4134_RS06880 (ST4134_06870) - 1327105..1328310 (+) 1206 WP_011681420.1 OFA family MFS transporter -
  ST4134_RS06885 (ST4134_06875) - 1328356..1329669 (-) 1314 Protein_1302 IS3 family transposase -
  ST4134_RS06890 (ST4134_06880) pta 1329794..1330777 (-) 984 WP_011227420.1 phosphate acetyltransferase -
  ST4134_RS06895 (ST4134_06885) - 1330791..1331687 (-) 897 WP_024703943.1 RluA family pseudouridine synthase -
  ST4134_RS06900 (ST4134_06890) - 1331684..1332520 (-) 837 WP_014608552.1 NAD kinase -
  ST4134_RS06905 (ST4134_06895) - 1332492..1333166 (-) 675 WP_011681427.1 GTP pyrophosphokinase family protein -
  ST4134_RS06910 (ST4134_06900) - 1333262..1333837 (+) 576 WP_002951444.1 CYTH domain-containing protein -
  ST4134_RS06915 (ST4134_06905) - 1334200..1335171 (+) 972 WP_002951446.1 ribose-phosphate diphosphokinase -
  ST4134_RS06920 (ST4134_06910) - 1335175..1336287 (+) 1113 WP_014608554.1 cysteine desulfurase family protein -
  ST4134_RS06925 (ST4134_06915) - 1336289..1336636 (+) 348 WP_014608555.1 DUF1831 domain-containing protein -
  ST4134_RS06930 (ST4134_06920) - 1336780..1337439 (+) 660 WP_024703944.1 redox-sensing transcriptional repressor Rex -
  ST4134_RS06935 (ST4134_06925) - 1337432..1338127 (+) 696 WP_024703945.1 gamma-glutamyl-gamma-aminobutyrate hydrolase family protein -
  ST4134_RS06940 (ST4134_06930) radC 1338180..1338866 (-) 687 WP_014727630.1 DNA repair protein RadC -
  ST4134_RS06945 (ST4134_06935) - 1338915..1340699 (-) 1785 WP_024703946.1 rhamnan synthesis F family protein -
  ST4134_RS06950 (ST4134_06940) - 1340696..1341763 (-) 1068 WP_024703947.1 glycosyltransferase -
  ST4134_RS06955 (ST4134_06945) - 1341783..1342988 (-) 1206 WP_014727633.1 ABC transporter ATP-binding protein -
  ST4134_RS06960 (ST4134_06950) - 1342988..1343797 (-) 810 WP_024703948.1 ABC transporter permease -
  ST4134_RS06965 (ST4134_06955) - 1343781..1344737 (-) 957 WP_024703949.1 glycosyltransferase family 2 protein -
  ST4134_RS06970 (ST4134_06960) cps2T 1344734..1345882 (-) 1149 WP_024703950.1 beta 1-4 rhamnosyltransferase Cps2T -
  ST4134_RS06975 (ST4134_06965) - 1345989..1347530 (-) 1542 WP_024703951.1 DUF2142 domain-containing protein -
  ST4134_RS06980 (ST4134_06970) - 1347537..1348547 (-) 1011 WP_024703952.1 glycosyltransferase -
  ST4134_RS06985 (ST4134_06975) - 1348547..1349821 (-) 1275 WP_024703953.1 lipopolysaccharide biosynthesis protein -
  ST4134_RS06990 (ST4134_06980) - 1349814..1350146 (-) 333 WP_011226332.1 DUF2304 domain-containing protein -
  ST4134_RS06995 (ST4134_06985) - 1350152..1350847 (-) 696 WP_024703954.1 glycosyltransferase family 2 protein -
  ST4134_RS07000 (ST4134_06990) rfbD 1350924..1351775 (-) 852 WP_014621820.1 dTDP-4-dehydrorhamnose reductase -
  ST4134_RS07005 (ST4134_06995) - 1351852..1353186 (-) 1335 WP_024703955.1 DUF6056 family protein -
  ST4134_RS07010 (ST4134_07000) - 1353299..1354225 (-) 927 WP_024703956.1 glycosyltransferase family 2 protein -
  ST4134_RS07015 (ST4134_07005) - 1354235..1354570 (-) 336 WP_024703957.1 metal-sulfur cluster assembly factor -
  ST4134_RS07020 (ST4134_07010) rpoD 1354624..1355733 (-) 1110 WP_011227440.1 RNA polymerase sigma factor RpoD -
  ST4134_RS07025 (ST4134_07015) dnaG 1355737..1357548 (-) 1812 WP_024703958.1 DNA primase -
  ST4134_RS07030 (ST4134_07020) mscL 1357691..1357771 (+) 81 Protein_1331 large conductance mechanosensitive channel protein MscL -
  ST4134_RS07035 (ST4134_07025) rpsU 1357931..1358107 (-) 177 WP_011681449.1 30S ribosomal protein S21 -
  ST4134_RS07040 (ST4134_07030) - 1358301..1359095 (-) 795 WP_023909836.1 ABC transporter substrate-binding protein -
  ST4134_RS07045 (ST4134_07035) - 1359157..1360266 (-) 1110 WP_024703959.1 aminotransferase -
  ST4134_RS07050 (ST4134_07040) - 1360614..1361411 (-) 798 WP_014621828.1 transporter substrate-binding domain-containing protein -
  ST4134_RS07055 (ST4134_07045) - 1361408..1362238 (-) 831 WP_014621829.1 transporter substrate-binding domain-containing protein -
  ST4134_RS07060 (ST4134_07050) - 1362452..1362916 (-) 465 WP_011681454.1 8-oxo-dGTP diphosphatase -
  ST4134_RS07065 (ST4134_07055) uvrB 1362961..1364952 (-) 1992 WP_014608579.1 excinuclease ABC subunit UvrB -
  ST4134_RS07070 (ST4134_07060) - 1365099..1365566 (-) 468 WP_014608580.1 hypothetical protein -
  ST4134_RS07075 (ST4134_07065) - 1365639..1366575 (-) 937 Protein_1340 CPBP family intramembrane glutamic endopeptidase -
  ST4134_RS07080 (ST4134_07070) - 1366774..1368984 (+) 2211 WP_014727645.1 ABC transporter substrate-binding protein/permease -
  ST4134_RS07085 (ST4134_07075) - 1368984..1369724 (+) 741 WP_024703961.1 amino acid ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 309 a.a.        Molecular weight: 35171.28 Da        Isoelectric Point: 6.5989

>NTDB_id=510074 ST4134_RS06825 WP_002951426.1 1314319..1315248(-) (amiF) [Streptococcus thermophilus strain 4134]
MPEKLVEVKDVEISFGEGRKKFVAVHNANFFINKGETFSLVGESGSGKTTIGRAIIGLNDTSNGEIIFDGKKINGYLSHS
EKNDLIRRIQMIFQDPAASLNERATVDYILSEGLYNFHLYKDEEERKAKIKEIIKEVGLLEEHLTRYPHEFSGGQRQRIG
IARSLVMQPDLVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFISDRIAVIYKGTIVEVAETEELYN
NPIHPYTKSLLSAVPIPDPILERKKVLKVYDPNQHDYSVDKPEMVEVRPGHFVWGNKTEIETYRKEQSK

Nucleotide


Download         Length: 930 bp        

>NTDB_id=510074 ST4134_RS06825 WP_002951426.1 1314319..1315248(-) (amiF) [Streptococcus thermophilus strain 4134]
ATGCCTGAGAAATTAGTTGAAGTAAAAGATGTGGAAATTTCCTTCGGCGAAGGAAGAAAGAAGTTCGTTGCTGTCCACAA
TGCTAATTTTTTCATCAACAAGGGTGAAACCTTCTCCCTCGTTGGTGAGTCTGGTAGTGGTAAAACGACTATTGGACGTG
CCATTATCGGTTTAAATGACACAAGTAATGGTGAGATTATTTTTGACGGTAAGAAGATCAATGGATACTTATCTCACTCT
GAGAAAAACGACCTTATCCGTCGTATTCAGATGATTTTCCAAGACCCTGCGGCTAGTTTGAATGAACGTGCGACAGTCGA
TTATATCTTGTCTGAGGGCTTGTACAATTTCCATCTTTATAAAGATGAGGAAGAACGTAAGGCTAAAATCAAGGAAATCA
TCAAAGAAGTAGGACTTCTTGAGGAGCACTTAACACGTTACCCTCACGAATTTTCTGGGGGACAACGTCAACGTATCGGG
ATTGCGCGTTCTTTGGTCATGCAGCCTGATTTGGTTATCGCTGATGAACCAATCTCAGCCCTTGACGTGTCAGTTCGTGC
CCAAGTTTTGAATTTGCTTAAGAAATTCCAAAAAGAGTTGGGGTTAACCTATCTCTTTATCGCTCACGATTTGTCAGTGG
TCCGTTTCATTTCTGATCGTATCGCTGTTATCTATAAGGGGACAATCGTGGAAGTTGCTGAGACAGAAGAGCTCTACAAC
AATCCTATCCATCCTTACACCAAGTCACTCTTGTCTGCTGTTCCTATTCCAGATCCAATCTTGGAACGTAAGAAAGTCTT
GAAGGTTTATGATCCAAACCAACACGACTATTCGGTTGATAAACCAGAAATGGTGGAAGTACGCCCAGGTCACTTCGTTT
GGGGTAACAAGACAGAAATTGAGACTTATCGTAAAGAACAAAGTAAGTAA

Domains

Predicted by InterproScan.

ABC transporter (ABC_tran)

(26-178)

Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY)

(230-262)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7U7CIU5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiF Streptococcus thermophilus LMG 18311

100

100

1
  amiF Streptococcus thermophilus LMD-9

99.676

100

0.997
  amiF Streptococcus salivarius strain HSISS4

98.058

100

0.981