Detailed information NTG0509952

Bioinformatically predicted

Overview

• Name: endA
• Type: Machinery gene
• Locus tag: ST4052_RS05250
• Genome accession: NZ_CP065476
• Coordinates: 1002204..1003085 (-)
• Length: 293 a.a.
• NCBI ID: WP_011226089.1
• Uniprot ID: Q5M441
• Organism: Streptococcus thermophilus strain 4052
• Function: cleavage of dsDNA into ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 997204..1008085

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ST4052_RS05225 (ST4052_05310)	vicR	997905..998612 (-)	708	WP_011227274.1	response regulator YycF	Regulator
ST4052_RS05230 (ST4052_05315)	-	999143..999910 (+)	768	WP_347100829.1	amino acid ABC transporter ATP-binding protein	-
ST4052_RS05235 (ST4052_05320)	-	999922..1000755 (+)	834	WP_011227276.1	transporter substrate-binding domain-containing protein	-
ST4052_RS05240 (ST4052_05325)	-	1000773..1001471 (+)	699	WP_011226087.1	amino acid ABC transporter permease	-
ST4052_RS05245 (ST4052_05330)	-	1001483..1002133 (+)	651	WP_011226088.1	amino acid ABC transporter permease	-
ST4052_RS05250 (ST4052_05335)	endA	1002204..1003085 (-)	882	WP_011226089.1	DNA/RNA non-specific endonuclease	Machinery gene
ST4052_RS05255 (ST4052_05340)	-	1003147..1003335 (-)	189	WP_011227277.1	DNA-directed RNA polymerase subunit beta	-
ST4052_RS05260 (ST4052_05345)	murA	1003337..1004608 (-)	1272	WP_041827046.1	UDP-N-acetylglucosamine 1-carboxyvinyltransferase	-
ST4052_RS05265 (ST4052_05350)	-	1004678..1004914 (-)	237	WP_002946959.1	DUF1146 family protein	-
ST4052_RS05270 (ST4052_05355)	galE	1005102..1006115 (-)	1014	WP_011227279.1	UDP-glucose 4-epimerase GalE	-
ST4052_RS05275 (ST4052_05360)	-	1006130..1006585 (-)	456	WP_041827047.1	DUF4832 domain-containing protein	-

Sequence

Protein

• Length: 293 a.a.
• Molecular weight: 31757.50 Da
• Isoelectric Point: 10.1789

>NTDB_id=509952 ST4052_RS05250 WP_011226089.1 1002204..1003085(-) (endA) [Streptococcus thermophilus strain 4052]
MAKRKTSLTNKQKRQLVTLIIAALIAVLGYLGTSNKLSPDNPIRQVASLVAGKSNKSIKSNGSTNSQSTPQEQLAETVMT
SSVKSQLGNSLEWNGAGAYIINRNKTNLNAKVSSQPYANNQVKTVQGQTVPTVANALLSKATRQYKNRQETGNGSTSWTP
AGWHQVQNLSGEYSHAVDRGHLLGYALIGGLKGFDASTSNPENIAVQTAWANQAYSDSSTGQNYYESLVRKALDKNKRVR
YRVTLIYEGENLIPSGTHLEAKSADGSLEFNVFVPNVQEGITLDYYSGKVTVN

Nucleotide

• Length: 882 bp

>NTDB_id=509952 ST4052_RS05250 WP_011226089.1 1002204..1003085(-) (endA) [Streptococcus thermophilus strain 4052]
ATGGCAAAAAGAAAGACATCTTTGACAAATAAACAGAAACGTCAGTTAGTCACATTAATAATAGCAGCTTTGATAGCTGT
TTTAGGTTACCTAGGAACCAGTAACAAGCTCTCGCCTGATAATCCTATCAGACAAGTAGCAAGCCTAGTTGCAGGGAAAT
CTAACAAATCCATTAAATCTAATGGTTCAACAAATAGTCAGTCTACACCACAAGAGCAGCTTGCTGAGACAGTAATGACT
AGTTCAGTCAAAAGCCAACTGGGTAATTCCTTGGAATGGAATGGAGCTGGGGCCTACATTATTAATAGAAATAAGACGAA
TCTAAATGCTAAGGTATCAAGCCAACCCTATGCTAATAACCAAGTCAAAACGGTTCAAGGACAAACTGTTCCTACAGTTG
CTAATGCCTTATTAAGTAAAGCAACTAGACAGTACAAAAATCGTCAAGAAACAGGGAATGGTTCAACCTCTTGGACACCA
GCTGGATGGCACCAGGTTCAAAATTTATCAGGAGAGTATAGCCATGCCGTAGATAGAGGCCATTTGCTAGGTTATGCACT
GATAGGTGGTTTGAAAGGATTTGATGCCTCGACTTCAAATCCAGAGAATATTGCTGTTCAAACTGCTTGGGCAAATCAAG
CATATAGCGATAGTTCTACAGGCCAAAACTATTATGAAAGCCTAGTACGAAAAGCCCTGGATAAAAACAAACGTGTTCGT
TATCGTGTGACCCTGATTTATGAAGGAGAGAATCTCATTCCATCAGGAACACATCTTGAAGCTAAGTCAGCAGACGGCTC
ACTTGAGTTTAATGTCTTTGTTCCAAATGTACAAGAGGGAATTACACTAGATTACTATTCAGGAAAAGTAACGGTGAATT
GA

Domains

Predicted by InterproScan.

• DNA/RNA non-specific endonuclease (Endonuclease_NS) (101-266)

3D structure

• AlphaFold DB: Q5M441

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
endA	Streptococcus pneumoniae Rx1	72.803	81.57	0.594
endA	Streptococcus pneumoniae D39	72.803	81.57	0.594
endA	Streptococcus pneumoniae R6	72.803	81.57	0.594
endA	Streptococcus pneumoniae TIGR4	72.803	81.57	0.594