Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   I5960_RS02690 Genome accession   NZ_CP065411
Coordinates   551701..553134 (-) Length   477 a.a.
NCBI ID   WP_035887975.1    Uniprot ID   -
Organism   Kosakonia radicincitans strain IAC-BECa95     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 546701..558134
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I5960_RS02660 elbB 548269..548922 (+) 654 WP_227136316.1 isoprenoid biosynthesis glyoxalase ElbB -
  I5960_RS02665 mtgA 548919..549650 (+) 732 WP_035942012.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  I5960_RS02670 npr 549652..549924 (-) 273 WP_007369893.1 PTS phosphocarrier protein NPr -
  I5960_RS02675 rapZ 549921..550775 (-) 855 WP_007369894.1 RNase adapter RapZ -
  I5960_RS02680 ptsN 550818..551309 (-) 492 WP_007369895.1 PTS IIA-like nitrogen regulatory protein PtsN -
  I5960_RS02685 hpf 551391..551678 (-) 288 WP_007369896.1 ribosome hibernation promoting factor -
  I5960_RS02690 rpoN/rpoN1 551701..553134 (-) 1434 WP_035887975.1 RNA polymerase factor sigma-54 Machinery gene
  I5960_RS02695 lptB 553183..553908 (-) 726 WP_007369898.1 LPS export ABC transporter ATP-binding protein -
  I5960_RS02700 lptA 553915..554469 (-) 555 WP_007369899.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  I5960_RS02705 lptC 554438..555013 (-) 576 WP_007369900.1 LPS export ABC transporter periplasmic protein LptC -
  I5960_RS02710 kdsC 555010..555576 (-) 567 WP_035887972.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  I5960_RS02715 kdsD 555591..556577 (-) 987 WP_007369902.1 arabinose-5-phosphate isomerase KdsD -
  I5960_RS02720 - 556591..557565 (-) 975 WP_007369903.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53802.57 Da        Isoelectric Point: 4.3889

>NTDB_id=509253 I5960_RS02690 WP_035887975.1 551701..553134(-) (rpoN/rpoN1) [Kosakonia radicincitans strain IAC-BECa95]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQADLHEEVDSQQTQENEGLDTVDALEQKEMPE
ELPLDASWDEIYTAGTPSGSSHDYIDDELPIYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVSLED
VLESMGDEEIGLDEVEAVLKRVQRFDPVGVAARDLRDCLLIQLSQFAKDTPWCEEARLIISDHLDLLANHDFRSLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGRWTVELNSDSIPRLQINQHYASMGGGARNDADTQFIR
SNLQEAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTLLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=509253 I5960_RS02690 WP_035887975.1 551701..553134(-) (rpoN/rpoN1) [Kosakonia radicincitans strain IAC-BECa95]
ATGAAGCAAGGTTTGCAATTAAGGCTTAGCCAACAGCTAGCTATGACGCCGCAACTGCAACAGGCTATCCGTCTGTTGCA
GCTGTCTACGTTAGAACTTCAGCAGGAACTTCAGCAAGCGCTGGAGAGTAACCCATTGCTGGAACAAGCCGATCTTCATG
AAGAAGTGGATAGCCAACAAACCCAGGAAAATGAAGGCCTGGATACTGTAGACGCGCTCGAACAGAAAGAGATGCCTGAA
GAGCTGCCGCTGGACGCCAGCTGGGATGAGATTTATACCGCCGGAACGCCTTCCGGCAGCAGCCACGATTATATTGATGA
CGAACTGCCGATTTATCAGGGTGAAACGACACAGTCTCTTCAGGATTACCTGATGTGGCAGGTAGAGTTAACGCCGTTTT
CCGATACCGATCGCGCAATCGCAACCTCTATTGTTGATGCTGTCGATGAGACCGGCTACCTTACCGTGTCGCTTGAGGAT
GTGCTGGAAAGCATGGGCGATGAAGAAATCGGTCTCGATGAAGTTGAAGCGGTGTTAAAGCGCGTTCAGCGTTTCGATCC
GGTTGGCGTCGCTGCGCGCGATCTGCGCGACTGCCTGTTGATCCAACTGTCCCAATTCGCGAAAGATACGCCCTGGTGTG
AAGAGGCTCGCCTGATTATCAGCGATCACCTTGATCTGCTCGCTAACCATGATTTCCGCTCTCTCATGCGCGTCACACGT
CTTAAAGAGGATGTGCTGAAAGAAGCGGTAAATCTGATCCAGTCGCTGGATCCGCGCCCCGGCCAGTCGATCCAGACCGG
TGAACCAGAATATGTCATCCCGGATGTGCTGGTGCGTAAGCACAACGGGCGCTGGACGGTAGAACTGAACTCCGATAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCTTCAATGGGCGGCGGCGCGCGCAATGACGCCGACACCCAGTTTATCCGC
AGTAACTTGCAGGAAGCGAAGTGGCTGATCAAAAGCCTTGAAAGCCGTAATGACACGCTATTGCGCGTCAGCCGCTGCAT
CGTCGAGCAGCAGCAGGCATTCTTTGAACAGGGCGAAGAGTTTATGAAACCGATGGTGCTGGCGGATATCGCTCAGGCCG
TCGAAATGCACGAGTCCACGATTTCCCGTGTGACAACGCAGAAGTACCTGCACAGTCCTCGCGGCATTTTTGAACTGAAG
TATTTCTTCTCCAGCCATGTGAATACGGAAGGCGGCGGCGAAGCCTCGTCAACAGCCATTCGCGCACTGGTTAAGAAATT
AATCGCCGCAGAAAACCCTGCAAAACCACTGAGCGACAGTAAGCTGACGACGCTACTTTCCGATCAAGGAATTATGGTAG
CGCGGCGTACCGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAGCTGGTGTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.747

100

0.482