Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   I5286_RS01915 Genome accession   NZ_CP065403
Coordinates   447217..448737 (-) Length   506 a.a.
NCBI ID   WP_054929657.1    Uniprot ID   -
Organism   Paraburkholderia caribensis strain IAC-BECa88     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 442217..453737
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I5286_RS01890 - 442994..443629 (-) 636 WP_054929655.1 LON peptidase substrate-binding domain-containing protein -
  I5286_RS01895 rapZ 443694..444587 (-) 894 WP_060599302.1 RNase adapter RapZ -
  I5286_RS01900 hprK 444665..445633 (-) 969 WP_007579699.1 HPr(Ser) kinase/phosphatase -
  I5286_RS01905 - 445867..446382 (-) 516 WP_028365018.1 PTS sugar transporter subunit IIA -
  I5286_RS01910 hpf 446682..447041 (-) 360 WP_012399744.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  I5286_RS01915 rpoN/rpoN1 447217..448737 (-) 1521 WP_054929657.1 RNA polymerase factor sigma-54 Machinery gene
  I5286_RS01920 lptB 448978..449760 (-) 783 WP_035986723.1 LPS export ABC transporter ATP-binding protein -
  I5286_RS01925 lptA 449757..450434 (-) 678 WP_054929658.1 lipopolysaccharide transport periplasmic protein LptA -
  I5286_RS01930 lptC 450456..451061 (-) 606 WP_054929659.1 LPS export ABC transporter periplasmic protein LptC -
  I5286_RS01935 - 451058..451618 (-) 561 WP_054929660.1 HAD family hydrolase -
  I5286_RS01940 kdsD 451618..452601 (-) 984 WP_054929661.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 506 a.a.        Molecular weight: 55526.41 Da        Isoelectric Point: 4.8325

>NTDB_id=509191 I5286_RS01915 WP_054929657.1 447217..448737(-) (rpoN/rpoN1) [Paraburkholderia caribensis strain IAC-BECa88]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVSMAIAQNPLLETEDDWIASPLRVAADGTVIAQTSQNTSPEEMS
GAPPSSSSSTSSGESQEGGEPQGVDEYNGMGSDANSDANQWNLDDYGRSGNASDDDDLPPLQIHESTTTLRDHLTAQLRV
TGASARDRALITFLIESLDDDGYLTATFDEILADLPEELEVDTDELNAALALLHSFDPAGVGARSASECLRLQLGRLEPS
PTRTLALDIVAHHLELLAARDFTRLRKYLKASDDDLREAHALIRSLEPFPGAAYGKAEADYVVPDIMVRKTAQGWQAELN
PEVVPKLRINHLYANILRNNRGDPGSGSLRQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREI
ADTLGLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDTKSPLSDSRIAELLAEQG
FVVARRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1521 bp        

>NTDB_id=509191 I5286_RS01915 WP_054929657.1 447217..448737(-) (rpoN/rpoN1) [Paraburkholderia caribensis strain IAC-BECa88]
ATGAAAGCCAGCCTCCAACTTCGCCTGTCGCAGCATCTTGCGCTGACACCACAACTGCAACAGTCCATCCGGCTGCTGCA
GCTGTCTACGCTCGAACTGCAACAGGAAGTGTCGATGGCGATCGCCCAGAATCCGTTGCTCGAAACGGAGGACGACTGGA
TCGCGAGTCCGTTGCGCGTCGCCGCCGACGGCACTGTCATCGCACAGACCTCGCAGAACACGTCGCCGGAAGAGATGTCG
GGCGCGCCGCCGTCCTCGTCGTCGTCGACGTCCTCCGGCGAAAGCCAGGAAGGCGGCGAGCCACAGGGTGTGGACGAATA
CAACGGCATGGGTTCGGATGCGAACTCGGACGCCAACCAGTGGAATCTCGACGACTACGGCCGTTCGGGCAACGCATCGG
ACGACGACGATCTGCCGCCGCTGCAAATCCACGAATCGACCACGACGCTGCGCGATCACCTGACTGCTCAACTTCGCGTA
ACGGGAGCAAGCGCACGGGACCGCGCGCTCATCACGTTCCTGATCGAATCGCTCGACGACGACGGCTATCTGACGGCCAC
CTTCGACGAAATCCTCGCCGATTTGCCCGAAGAACTCGAAGTCGATACCGACGAACTCAACGCGGCCCTCGCCTTGCTGC
ACAGCTTCGACCCAGCGGGGGTCGGCGCGCGTTCGGCGTCGGAATGCCTGCGTTTGCAGTTGGGCCGGCTCGAGCCGTCG
CCCACGCGCACGCTCGCGCTCGACATCGTCGCGCATCACCTGGAACTGCTCGCCGCACGCGACTTCACGCGACTGCGCAA
ATACCTGAAGGCAAGCGACGACGATCTGCGCGAAGCGCATGCGCTGATCCGCTCGCTGGAGCCGTTCCCCGGTGCGGCCT
ACGGCAAGGCCGAGGCGGACTACGTCGTGCCCGACATCATGGTGCGCAAAACGGCACAGGGATGGCAGGCCGAACTGAAT
CCGGAAGTCGTGCCGAAACTGCGTATCAACCATCTGTACGCGAATATCCTGCGCAACAATCGAGGCGATCCGGGCAGCGG
TTCGCTGCGGCAGCAACTGCAGGAAGCGCGTTGGCTGATCAAGAATATCCAGCAGCGCTTCGAGACGATCCTGCGTGTCG
CACAGGCAATTGTGGAGCGTCAAAAGAACTTTTTCGCCCACGGCGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATA
GCTGATACGCTGGGTTTACATGAGTCGACGGTCTCGCGTGTGACAACCGGCAAGTACATGCTCACCCCATTCGGGACGCT
TGAATTTAAGTACTTCTTCGGATCGCACGTGTCCACCGACACGGGGGGCGCGGCCTCTTCAACGGCCATTCGCGCACTCA
TCAAGCAACTGATAGGAGCGGAAGACACCAAATCTCCTCTTTCAGACAGCCGCATTGCCGAACTGCTGGCGGAACAGGGT
TTCGTCGTCGCTCGCCGTACCGTTGCGAAATACCGCGAAGCACTGAAAATCCCGGCAGTCAATCTGCGCAAGTCTCTTTA
G

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(143-333)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(349-504)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

66.996

100

0.67