Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   I5Z97_RS02525 Genome accession   NZ_CP065386
Coordinates   486180..486761 (-) Length   193 a.a.
NCBI ID   WP_005710402.1    Uniprot ID   A0A143CGR5
Organism   Glaesserella parasuis strain LHDR_HPS_1_2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 481180..491761
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I5Z97_RS02485 (I5Z97_02485) - 481523..482161 (+) 639 WP_035496518.1 nitroreductase family protein -
  I5Z97_RS02490 (I5Z97_02490) - 482250..482783 (+) 534 WP_010786060.1 TIGR00645 family protein -
  I5Z97_RS02495 (I5Z97_02495) - 482870..484168 (-) 1299 WP_005710399.1 NAD(P)/FAD-dependent oxidoreductase -
  I5Z97_RS02515 (I5Z97_02515) greB 484696..485184 (-) 489 WP_035496521.1 transcription elongation factor GreB -
  I5Z97_RS02520 (I5Z97_02520) - 485184..486119 (-) 936 WP_005710401.1 KpsF/GutQ family sugar-phosphate isomerase -
  I5Z97_RS02525 (I5Z97_02525) pilF 486180..486761 (-) 582 WP_005710402.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  I5Z97_RS02530 (I5Z97_02530) - 486861..488021 (-) 1161 WP_010786053.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  I5Z97_RS02535 (I5Z97_02535) - 488222..488530 (-) 309 WP_005710405.1 helix-turn-helix domain-containing protein -
  I5Z97_RS02540 (I5Z97_02540) - 488534..488872 (-) 339 WP_062923967.1 toxin -
  I5Z97_RS02565 (I5Z97_02565) gltX 489711..491150 (+) 1440 WP_035496906.1 glutamate--tRNA ligase -

Sequence

Protein


Download         Length: 193 a.a.        Molecular weight: 22324.27 Da        Isoelectric Point: 8.0872

>NTDB_id=509121 I5Z97_RS02525 WP_005710402.1 486180..486761(-) (pilF) [Glaesserella parasuis strain LHDR_HPS_1_2]
MTFAKFFRNLTACSVALWLVACANQTTQVDFNRSEAVKARINLALAYLEQSDFPKAKENIDKALEHDVKDYLPHSVLAYY
YQQTGDNQKAEEAYQQALKLSKTQSKNNQVRPDVLNNYGTFLCKQKLFDKAYQQFETALTSQEAYYNQADTLENIALCAN
MKKDIEKQKTALSQLEKIDKSRAERLYNFMEIR

Nucleotide


Download         Length: 582 bp        

>NTDB_id=509121 I5Z97_RS02525 WP_005710402.1 486180..486761(-) (pilF) [Glaesserella parasuis strain LHDR_HPS_1_2]
ATGACCTTTGCAAAATTTTTCCGTAATTTGACCGCTTGTAGCGTGGCATTATGGCTTGTTGCTTGTGCGAACCAAACTAC
TCAGGTGGATTTTAACCGCTCGGAAGCCGTCAAAGCTCGCATTAATTTGGCCCTTGCTTACCTTGAACAATCTGATTTTC
CAAAAGCGAAAGAAAACATTGACAAAGCGCTTGAACACGATGTAAAAGACTACCTACCGCACTCTGTGCTTGCCTATTAT
TACCAACAAACTGGCGATAACCAAAAAGCAGAAGAAGCCTACCAGCAAGCCTTGAAGTTAAGTAAAACACAGAGCAAAAA
CAATCAAGTTCGCCCTGATGTATTAAATAACTACGGTACCTTTCTGTGCAAACAAAAGCTGTTCGACAAAGCTTATCAGC
AATTTGAAACGGCATTAACCAGTCAAGAAGCCTATTACAATCAAGCAGATACACTAGAAAATATCGCCTTATGTGCCAAT
ATGAAGAAGGATATTGAGAAGCAAAAAACAGCACTATCTCAACTTGAGAAAATAGATAAATCAAGAGCAGAACGACTATA
TAATTTCATGGAGATTAGATAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_2)

(74-102)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A143CGR5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

100

100

1
  pilF/pilF2 Haemophilus influenzae Rd KW20

44.693

92.746

0.415