Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   I5Q59_RS28585 Genome accession   NZ_CP065375
Coordinates   7225261..7226379 (-) Length   372 a.a.
NCBI ID   WP_011555738.1    Uniprot ID   Q1D099
Organism   Myxococcus xanthus strain DK1622::pDPO-Mxn116-Pvan-Tpase     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 7220261..7231379
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I5Q59_RS28570 (I5Q59_28555) - 7220923..7222359 (-) 1437 WP_011555735.1 sigma-54 dependent transcriptional regulator -
  I5Q59_RS28575 (I5Q59_28560) - 7222356..7223888 (-) 1533 WP_020478449.1 nitrogen regulation protein NR(II) -
  I5Q59_RS28580 (I5Q59_28565) - 7223950..7225203 (-) 1254 WP_011555737.1 type II secretion system F family protein -
  I5Q59_RS28585 (I5Q59_28570) pilT 7225261..7226379 (-) 1119 WP_011555738.1 type IV pilus twitching motility protein PilT Machinery gene
  I5Q59_RS28590 (I5Q59_28575) pilB 7226422..7228122 (-) 1701 WP_011555739.1 type IV-A pilus assembly ATPase PilB -
  I5Q59_RS28595 (I5Q59_28580) - 7228327..7229253 (-) 927 WP_011555740.1 bifunctional riboflavin kinase/FAD synthetase -
  I5Q59_RS28600 (I5Q59_28585) - 7229329..7229949 (+) 621 WP_011555741.1 tRNA (guanosine(46)-N(7))-methyltransferase TrmB -
  I5Q59_RS28605 (I5Q59_28590) - 7230060..7231103 (+) 1044 WP_011555742.1 diguanylate cyclase -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 40624.97 Da        Isoelectric Point: 8.2933

>NTDB_id=508962 I5Q59_RS28585 WP_011555738.1 7225261..7226379(-) (pilT) [Myxococcus xanthus strain DK1622::pDPO-Mxn116-Pvan-Tpase]
MANLHQLLKAMVEKGASDLHVTTGSPPQLRVDGELVPLKTAPLTPVETKQLCYSILTDAQKHKFEEDNELDLSFGVKGLS
RFRANIFMQRGAVAAAFRTIPFKILTFQELGLPPVVAELVKKPRGLILVTGPTGSGKSTTLASMIDKINTERHEHIMTIE
DPIEYLHPHKNCLVNQREVGADTRNFKTALKYILRQDPDVVLVGELRDLETIEAALTIAETGHICYATLHTNSAVQTINR
VLDVFPPYQQPQVRAQMSFVLEGVMSQALVAKAGGPGRILALEVMVPNPAIRNLIREDKVHQIYSSMQVGQAKFGMQTFN
QALAALLLRRLITQDEAFGRSSDAEELRNILATGGGGALPGMQRPGGGAGGR

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=508962 I5Q59_RS28585 WP_011555738.1 7225261..7226379(-) (pilT) [Myxococcus xanthus strain DK1622::pDPO-Mxn116-Pvan-Tpase]
GTGGCCAACCTGCACCAGCTCCTCAAGGCGATGGTCGAGAAGGGCGCTTCCGACCTCCACGTCACCACCGGCTCTCCGCC
GCAGCTTCGCGTCGACGGCGAGCTCGTCCCCTTGAAGACGGCGCCGCTGACCCCGGTGGAGACGAAGCAGCTCTGCTACT
CCATCCTCACGGACGCCCAGAAGCACAAGTTCGAAGAGGACAACGAGCTGGACCTGTCCTTCGGCGTGAAGGGCCTGTCG
CGCTTCCGCGCCAACATCTTCATGCAGCGTGGCGCGGTCGCCGCGGCGTTCCGGACCATTCCCTTCAAGATTTTGACGTT
CCAGGAGCTGGGCCTGCCGCCGGTGGTGGCGGAGCTGGTGAAGAAGCCCCGCGGGCTCATCCTGGTGACGGGCCCCACGG
GTTCGGGCAAGTCCACCACGCTGGCCTCGATGATCGACAAGATCAACACCGAGCGTCATGAGCACATCATGACCATCGAG
GACCCCATCGAGTACCTGCACCCGCACAAGAACTGCCTCGTGAATCAGCGCGAGGTGGGGGCGGACACGCGGAACTTCAA
GACGGCCCTCAAGTACATCCTCCGCCAGGACCCGGACGTGGTGCTCGTCGGTGAGCTCCGTGACCTGGAGACGATTGAAG
CGGCGCTCACCATCGCGGAGACGGGCCACATCTGCTACGCGACGCTACACACCAATAGCGCGGTGCAGACCATCAACCGC
GTGTTGGACGTGTTCCCGCCGTACCAGCAGCCGCAGGTCCGCGCGCAGATGTCCTTCGTGCTGGAAGGCGTGATGAGCCA
GGCGCTGGTGGCGAAGGCGGGCGGCCCCGGCCGCATCCTGGCGCTGGAGGTGATGGTGCCCAACCCCGCAATCCGGAACC
TGATCCGCGAGGACAAGGTCCACCAGATCTACTCGTCCATGCAGGTCGGCCAGGCGAAGTTCGGCATGCAGACCTTCAAC
CAGGCCCTGGCGGCGCTGCTGCTGCGGCGGCTCATCACCCAGGACGAGGCCTTCGGACGCTCTAGCGACGCGGAGGAGTT
GCGCAACATCCTGGCCACGGGCGGCGGCGGGGCCTTGCCCGGAATGCAGCGGCCAGGCGGGGGAGCGGGTGGTCGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q1D099

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.975

96.505

0.492
  pilT Legionella pneumophila strain ERS1305867

52.836

90.054

0.476
  pilT Legionella pneumophila strain Lp02

52.836

90.054

0.476
  pilT Pseudomonas aeruginosa PAK

52.239

90.054

0.47
  pilT Pseudomonas stutzeri DSM 10701

52.239

90.054

0.47
  pilT Acinetobacter baumannii D1279779

51.964

88.978

0.462
  pilT Acinetobacter baumannii strain A118

51.964

88.978

0.462
  pilT Acinetobacter nosocomialis M2

51.964

88.978

0.462
  pilT Acinetobacter baylyi ADP1

52.121

88.71

0.462
  pilT Vibrio cholerae strain A1552

48.503

89.785

0.435
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.503

89.785

0.435
  pilT Neisseria meningitidis 8013

44.868

91.667

0.411
  pilT Neisseria gonorrhoeae MS11

44.868

91.667

0.411
  pilU Vibrio cholerae strain A1552

43.059

94.892

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.026

94.355

0.387
  pilU Acinetobacter baylyi ADP1

39.481

93.28

0.368