NTDB

Overview

Name	pilA	Type	Machinery gene
Locus tag	I3251_RS10685	Genome accession	NZ_CP065374
Coordinates	2261424..2261876 (-)	Length	150 a.a.
NCBI ID	WP_023518088.1	Uniprot ID	-
Organism	Pseudomonas aeruginosa strain PAG7
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2256424..2266876

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I3251_RS10660 (I3251_10660)	-	2256563..2257274 (+)	712	Protein_2114	helix-turn-helix transcriptional regulator	-
I3251_RS10665 (I3251_10665)	-	2257444..2257686 (-)	243	Protein_2115	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
I3251_RS10670 (I3251_10670)	-	2257837..2260092 (-)	2256	WP_003104913.1	DUF1631 domain-containing protein	-
I3251_RS10675 (I3251_10675)	nadC	2260378..2261226 (+)	849	WP_003116246.1	carboxylating nicotinate-nucleotide diphosphorylase	-
I3251_RS10685 (I3251_10685)	pilA	2261424..2261876 (-)	453	WP_023518088.1	pilin	Machinery gene
I3251_RS10690 (I3251_10690)	pilB	2262108..2263808 (+)	1701	WP_021264132.1	type IV-A pilus assembly ATPase PilB	Machinery gene
I3251_RS10695 (I3251_10695)	pilC	2263812..2265032 (+)	1221	WP_023101565.1	type 4a pilus biogenesis protein PilC	Machinery gene
I3251_RS10700 (I3251_10700)	pilD	2265036..2265908 (+)	873	WP_009875878.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
I3251_RS10705 (I3251_10705)	coaE	2265905..2266516 (+)	612	WP_003112838.1	dephospho-CoA kinase	-
I3251_RS10710 (I3251_10710)	yacG	2266513..2266713 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-

Sequence

Protein

Download Length: 150 a.a. Molecular weight: 15686.02 Da Isoelectric Point: 7.7814

                                >NTDB_id=508924 I3251_RS10685 WP_023518088.1 2261424..2261876(-) (pilA) [Pseudomonas aeruginosa strain PAG7]

MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGSKILIGTTASTADTTYV

GIDEKANKLGTVAVAIKDTGDGTVKFTFATGQSSPKNAGTEITLNRTAEGVWTCTSTQEEMFIPKGCNKP

Nucleotide

Download Length: 453 bp

                                >NTDB_id=508924 I3251_RS10685 WP_023518088.1 2261424..2261876(-) (pilA) [Pseudomonas aeruginosa strain PAG7]

ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCGATTGCCAT

TCCCCAGTATCAGAACTATGTTGCGCGTTCGGAAGGTGCTTCGGCGCTGGCGACGATCAACCCGTTGAAGACCACTGTTG

AAGAGTCGCTGTCGCGTGGAATTGCTGGTAGCAAAATTCTGATCGGTACTACAGCTTCTACTGCAGATACCACCTATGTA

GGTATTGATGAGAAGGCAAATAAACTTGGTACCGTAGCTGTAGCTATTAAAGACACAGGCGATGGTACTGTAAAATTTAC

TTTTGCAACTGGTCAGTCCAGTCCGAAGAATGCGGGCACGGAAATTACTTTGAATCGTACTGCTGAAGGTGTATGGACTT

GCACCTCTACTCAGGAAGAGATGTTTATTCCTAAGGGTTGTAATAAGCCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-148)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA	Pseudomonas aeruginosa PAK	65.789	100	0.667
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	47.651	99.333	0.473
pilA	Vibrio cholerae strain A1552	47.651	99.333	0.473
pilA	Vibrio cholerae C6706	47.651	99.333	0.473
pilA/pilAI	Pseudomonas stutzeri DSM 10701	45.333	100	0.453
pilA	Acinetobacter baumannii strain A118	44.595	98.667	0.44
pilA	Vibrio parahaemolyticus RIMD 2210633	43.243	98.667	0.427
comP	Acinetobacter baylyi ADP1	42	100	0.42
pilA/pilAII	Pseudomonas stutzeri DSM 10701	41.958	95.333	0.4
pilA2	Legionella pneumophila strain ERS1305867	39.597	99.333	0.393
pilA2	Legionella pneumophila str. Paris	38.926	99.333	0.387
pilA/pilA1	Eikenella corrodens VA1	38.514	98.667	0.38
pilA	Ralstonia pseudosolanacearum GMI1000	38.889	96	0.373
pilA	Acinetobacter nosocomialis M2	41.353	88.667	0.367

Detailed information NTG0508924