Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   I5186_RS02605 Genome accession   NZ_CP065358
Coordinates   540152..541585 (-) Length   477 a.a.
NCBI ID   WP_035887975.1    Uniprot ID   -
Organism   Kosakonia oryzae strain IAC-BECa90     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 535152..546585
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I5186_RS02575 (I5186_02570) elbB 536720..537373 (+) 654 WP_227122236.1 isoprenoid biosynthesis glyoxalase ElbB -
  I5186_RS02580 (I5186_02575) mtgA 537370..538101 (+) 732 WP_227122237.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  I5186_RS02585 (I5186_02580) npr 538103..538375 (-) 273 WP_227122238.1 PTS phosphocarrier protein NPr -
  I5186_RS02590 (I5186_02585) rapZ 538372..539226 (-) 855 WP_007369894.1 RNase adapter RapZ -
  I5186_RS02595 (I5186_02590) ptsN 539269..539760 (-) 492 WP_007369895.1 PTS IIA-like nitrogen regulatory protein PtsN -
  I5186_RS02600 (I5186_02595) hpf 539842..540129 (-) 288 WP_227122239.1 ribosome hibernation promoting factor -
  I5186_RS02605 (I5186_02600) rpoN/rpoN1 540152..541585 (-) 1434 WP_035887975.1 RNA polymerase factor sigma-54 Machinery gene
  I5186_RS02610 (I5186_02605) lptB 541634..542359 (-) 726 WP_007369898.1 LPS export ABC transporter ATP-binding protein -
  I5186_RS02615 (I5186_02610) lptA 542366..542920 (-) 555 WP_043956378.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  I5186_RS02620 (I5186_02615) lptC 542889..543464 (-) 576 WP_043956379.1 LPS export ABC transporter periplasmic protein LptC -
  I5186_RS02625 (I5186_02620) kdsC 543461..544027 (-) 567 WP_043956380.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  I5186_RS02630 (I5186_02625) kdsD 544042..545028 (-) 987 WP_064563372.1 arabinose-5-phosphate isomerase KdsD -
  I5186_RS02635 (I5186_02630) - 545042..546016 (-) 975 WP_227122240.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53802.57 Da        Isoelectric Point: 4.3889

>NTDB_id=508839 I5186_RS02605 WP_035887975.1 540152..541585(-) (rpoN/rpoN1) [Kosakonia oryzae strain IAC-BECa90]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQADLHEEVDSQQTQENEGLDTVDALEQKEMPE
ELPLDASWDEIYTAGTPSGSSHDYIDDELPIYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVSLED
VLESMGDEEIGLDEVEAVLKRVQRFDPVGVAARDLRDCLLIQLSQFAKDTPWCEEARLIISDHLDLLANHDFRSLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGRWTVELNSDSIPRLQINQHYASMGGGARNDADTQFIR
SNLQEAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTLLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=508839 I5186_RS02605 WP_035887975.1 540152..541585(-) (rpoN/rpoN1) [Kosakonia oryzae strain IAC-BECa90]
ATGAAGCAAGGTTTGCAATTAAGGCTTAGCCAACAGCTAGCTATGACGCCGCAACTGCAACAGGCTATCCGTCTGTTGCA
GCTGTCTACGTTAGAACTTCAGCAGGAACTTCAGCAAGCGCTGGAGAGTAACCCATTGCTGGAACAAGCCGATCTTCATG
AAGAAGTGGATAGCCAACAAACCCAGGAAAATGAAGGCCTGGATACCGTAGACGCGCTCGAACAGAAAGAGATGCCTGAA
GAGCTGCCGCTGGACGCCAGTTGGGATGAGATTTATACCGCCGGAACGCCTTCCGGCAGCAGCCACGATTATATCGATGA
CGAACTGCCGATTTATCAGGGGGAAACGACACAATCTCTTCAAGATTACCTGATGTGGCAGGTGGAGTTAACGCCGTTTT
CCGATACCGACCGCGCAATCGCAACCTCTATTGTCGATGCTGTCGATGAGACCGGCTATCTTACCGTGTCGCTTGAGGAT
GTGCTGGAAAGCATGGGTGATGAAGAGATCGGTCTCGATGAAGTTGAAGCGGTGTTAAAGCGCGTTCAGCGTTTCGATCC
TGTCGGCGTCGCTGCGCGCGATCTGCGCGACTGCCTGTTGATCCAACTTTCCCAGTTTGCGAAAGATACGCCCTGGTGCG
AAGAGGCTCGCCTGATCATCAGCGATCACCTCGATCTGCTCGCTAACCACGATTTCCGCTCTCTTATGCGCGTCACGCGC
CTTAAAGAAGACGTGCTGAAAGAAGCGGTCAATCTGATCCAGTCGCTGGATCCGCGCCCCGGCCAGTCGATTCAGACCGG
CGAACCAGAATATGTCATCCCGGATGTTTTGGTGCGTAAGCATAACGGGCGCTGGACGGTAGAATTGAATTCCGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCTTCAATGGGCGGCGGCGCGCGCAATGACGCCGACACCCAGTTTATCCGC
AGTAATTTGCAGGAAGCGAAGTGGCTGATTAAAAGCCTTGAAAGCCGTAACGACACGCTATTGCGCGTCAGCCGCTGCAT
CGTCGAGCAGCAGCAGGCATTCTTTGAACAGGGCGAAGAGTTTATGAAACCGATGGTGCTGGCGGATATCGCCCAGGCCG
TCGAAATGCACGAGTCCACGATTTCCCGTGTGACAACGCAGAAGTACCTGCACAGTCCTCGCGGCATTTTTGAATTGAAG
TATTTCTTCTCCAGCCATGTGAATACGGAAGGCGGCGGCGAAGCCTCGTCGACAGCCATTCGCGCGCTGGTTAAGAAATT
AATCGCCGCAGAAAACCCTGCAAAACCACTGAGCGACAGTAAGCTGACGACGCTGCTTTCCGATCAAGGAATCATGGTAG
CGCGGCGTACCGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAGCTGGTGTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.747

100

0.482