Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   I3X02_RS22150 Genome accession   NZ_CP065213
Coordinates   4266055..4267107 (-) Length   350 a.a.
NCBI ID   WP_013059177.1    Uniprot ID   A0A8D4BHW7
Organism   Priestia megaterium strain HGS7     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4261055..4272107
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I3X02_RS22110 - 4262071..4262895 (+) 825 WP_209367848.1 YqhG family protein -
  I3X02_RS22115 - 4262919..4263104 (-) 186 WP_013059170.1 YqzE family protein -
  I3X02_RS22120 comGG 4263148..4263522 (-) 375 WP_182420451.1 competence type IV pilus minor pilin ComGG -
  I3X02_RS22125 comGF 4263512..4263949 (-) 438 WP_182420452.1 competence type IV pilus minor pilin ComGF -
  I3X02_RS22130 - 4263946..4264284 (-) 339 WP_028411610.1 type II secretion system protein -
  I3X02_RS22135 comGD 4264268..4264750 (-) 483 WP_226554895.1 competence type IV pilus minor pilin ComGD -
  I3X02_RS22140 comGC 4264692..4265003 (-) 312 WP_013059175.1 competence type IV pilus major pilin ComGC -
  I3X02_RS22145 comGB 4265018..4266064 (-) 1047 WP_014458228.1 competence type IV pilus assembly protein ComGB -
  I3X02_RS22150 comGA 4266055..4267107 (-) 1053 WP_013059177.1 competence type IV pilus ATPase ComGA Machinery gene
  I3X02_RS22155 - 4267377..4267760 (-) 384 WP_013059178.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  I3X02_RS22160 - 4268022..4268258 (+) 237 WP_013059179.1 DUF2626 domain-containing protein -
  I3X02_RS22165 - 4268296..4268928 (-) 633 WP_014458225.1 MBL fold metallo-hydrolase -
  I3X02_RS22170 - 4269137..4269313 (+) 177 WP_013059181.1 DUF2759 domain-containing protein -
  I3X02_RS22175 - 4269349..4270251 (-) 903 WP_209367851.1 M14 family metallocarboxypeptidase -
  I3X02_RS22180 - 4270363..4271331 (-) 969 WP_013059183.1 ROK family glucokinase -
  I3X02_RS22185 - 4271334..4271540 (-) 207 WP_013059184.1 YqgQ family protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39625.02 Da        Isoelectric Point: 9.0586

>NTDB_id=508157 I3X02_RS22150 WP_013059177.1 4266055..4267107(-) (comGA) [Priestia megaterium strain HGS7]
MTEIEQFATYIIRDACECRSSDVHIVPRKKDAVIQLRIDQELILKRVISKEMSERLISHFKFLGGMDIGERRKPQNGALT
TSIDHYQVNLRLSTLPTSHDESLVIRLLPHQQSTSLKHLSLFPEHTKKLISLLKFSHGLIIFTGPTGSGKTTTLYTLLHE
VKHLFNRNVITLEDPIEKPSETVLQVQVNERAGITYTTGLKAILRHDPDIIMVGEIRDSETAKIAVRAALTGHLVLTTMH
TKDTKGSLYRLLEFGVSFQEMEQTLVAVAAQRLVEIRCPLCSGKCHPACKKMRKHRQSSIYELLYGKELSAVMREVKGEN
ADYDYKTLRDVILKGIALGYLYPHSLDGWG

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=508157 I3X02_RS22150 WP_013059177.1 4266055..4267107(-) (comGA) [Priestia megaterium strain HGS7]
TTGACCGAAATTGAACAGTTTGCAACTTACATTATACGAGATGCATGTGAGTGCCGAAGTTCTGATGTGCATATTGTGCC
CCGAAAGAAAGATGCTGTGATTCAGCTACGAATTGACCAAGAGCTTATTTTAAAGAGGGTTATTTCGAAGGAAATGTCTG
AACGGCTCATTTCACATTTTAAATTTTTAGGGGGAATGGATATTGGCGAGCGCAGAAAACCTCAAAATGGTGCACTGACC
ACTTCCATTGATCACTATCAAGTAAATTTGCGTTTATCAACTCTTCCTACTTCTCATGATGAAAGTCTTGTTATTCGGCT
TTTGCCTCATCAACAATCTACATCTCTCAAACATTTATCTCTTTTTCCTGAACATACTAAAAAGCTTATCTCTCTCTTGA
AATTCTCTCATGGATTAATTATTTTTACCGGTCCTACAGGATCGGGTAAAACCACTACTCTATATACTCTTTTACACGAA
GTAAAGCATCTTTTTAATCGAAATGTTATTACACTAGAAGATCCTATTGAAAAACCGAGTGAAACCGTGCTTCAAGTACA
GGTAAATGAACGGGCGGGGATTACGTACACGACTGGTTTAAAAGCTATATTGCGCCATGACCCAGATATTATCATGGTGG
GAGAAATAAGAGATAGTGAGACTGCCAAAATTGCTGTGAGAGCAGCGCTTACCGGACACTTGGTTTTGACAACGATGCAT
ACTAAAGATACAAAAGGATCTTTGTACAGGCTGCTTGAGTTTGGTGTTAGCTTTCAAGAAATGGAACAGACTCTTGTAGC
AGTAGCTGCACAGCGCCTTGTAGAAATTCGCTGTCCGCTGTGTTCCGGAAAATGCCACCCAGCTTGTAAAAAGATGAGGA
AGCATCGTCAGTCCAGCATATACGAATTGCTTTATGGCAAGGAGCTTAGCGCGGTGATGAGAGAAGTGAAAGGAGAAAAT
GCGGATTATGATTATAAGACGCTCCGAGATGTTATTCTAAAAGGAATTGCGCTAGGTTATTTATATCCGCATTCGCTAGA
CGGATGGGGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

58.571

100

0.586