Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   I3X02_RS00535 Genome accession   NZ_CP065213
Coordinates   100370..101746 (+) Length   458 a.a.
NCBI ID   WP_208732805.1    Uniprot ID   -
Organism   Priestia megaterium strain HGS7     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 95370..106746
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I3X02_RS00515 - 95662..96123 (+) 462 WP_013054895.1 CtsR family transcriptional regulator -
  I3X02_RS00520 - 96165..96707 (+) 543 WP_014462042.1 UvrB/UvrC motif-containing protein -
  I3X02_RS00525 - 96711..97784 (+) 1074 WP_013054897.1 protein arginine kinase -
  I3X02_RS00530 clpC 97805..100246 (+) 2442 WP_014462041.1 ATP-dependent protease ATP-binding subunit ClpC -
  I3X02_RS00535 radA 100370..101746 (+) 1377 WP_208732805.1 DNA repair protein RadA Machinery gene
  I3X02_RS00540 disA 101750..102829 (+) 1080 WP_013054900.1 DNA integrity scanning diadenylate cyclase DisA -
  I3X02_RS00545 - 102977..104074 (+) 1098 WP_013054901.1 PIN/TRAM domain-containing protein -
  I3X02_RS00550 ispD 104100..104801 (+) 702 WP_116741825.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  I3X02_RS00555 ispF 104798..105277 (+) 480 WP_013054903.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49488.85 Da        Isoelectric Point: 6.9049

>NTDB_id=508125 I3X02_RS00535 WP_208732805.1 100370..101746(+) (radA) [Priestia megaterium strain HGS7]
MAKRKTKFACQHCGYESAKWMGKCPGCGSWNSMVEEMEETKSSRRGAFSGATASKVQKPQSITAIESTTEPRIFTPSAEL
NRVLGGGIVRGSLVLIGGDPGIGKSTLLLQTSAQLAMKQNKVLYISGEESTKQTKLRADRLGIKAEELYVHAETNLELIL
EAISSMQPDFVVIDSIQTIYHADVTSAPGSVSQVRECTAELMRVAKTNGIAIFIVGHVTKEGAIAGPRLLEHMVDTVLYF
EGERHHTYRILRAVKNRFGSTNEMGIFEMKESGLEEVLNPSEIFLEERSQGAAGSVVVASMEGTRPVLVELQALISPTSF
GNPRRMATGVDHNRVSLIMAVLEKRVGMLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDAASSATDVIVGEVGLT
GEVRRVSRIEQRVQEAVKLGFQRIIIPEKNLGGWKVPDGIDVIGVSTVAEALQYTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=508125 I3X02_RS00535 WP_208732805.1 100370..101746(+) (radA) [Priestia megaterium strain HGS7]
ATGGCGAAACGAAAAACAAAATTTGCATGTCAGCATTGCGGATATGAATCCGCAAAATGGATGGGGAAATGTCCCGGATG
CGGAAGCTGGAATTCGATGGTGGAAGAAATGGAAGAAACGAAATCATCACGCCGAGGAGCTTTTTCCGGAGCTACTGCTT
CTAAAGTTCAAAAACCTCAGTCAATTACAGCGATTGAATCTACAACAGAACCACGGATTTTTACACCTTCTGCGGAGTTA
AACCGTGTATTAGGAGGCGGTATTGTCAGAGGGTCACTCGTTTTAATAGGGGGAGATCCGGGTATCGGAAAGTCTACTTT
ACTTTTGCAAACCTCCGCGCAGTTAGCAATGAAACAAAATAAAGTACTATATATTTCGGGAGAGGAATCAACCAAACAAA
CGAAGTTAAGAGCGGACAGGCTAGGTATAAAAGCTGAAGAGCTTTATGTTCACGCAGAAACAAACTTAGAATTAATACTA
GAGGCCATCTCAAGTATGCAGCCCGATTTTGTGGTGATTGATTCAATTCAAACCATTTACCATGCTGATGTGACATCAGC
GCCAGGAAGCGTCTCACAGGTGCGAGAGTGTACGGCTGAACTAATGCGTGTTGCTAAAACAAATGGCATTGCCATCTTTA
TTGTGGGTCATGTAACGAAAGAAGGGGCCATTGCGGGTCCAAGACTTCTTGAACATATGGTGGATACGGTTCTTTATTTT
GAAGGAGAGCGTCATCATACGTATCGTATATTAAGAGCGGTTAAGAACCGATTTGGATCTACAAATGAAATGGGCATTTT
TGAAATGAAAGAAAGTGGGCTAGAAGAAGTATTGAATCCATCTGAAATCTTTCTAGAAGAACGGTCTCAGGGAGCAGCAG
GGTCGGTTGTTGTCGCTTCAATGGAGGGAACAAGACCCGTATTAGTAGAGCTGCAGGCGCTAATCAGTCCAACGAGCTTT
GGAAACCCTAGAAGAATGGCGACAGGAGTAGATCATAATCGCGTTTCGCTTATCATGGCTGTCTTAGAAAAGCGTGTAGG
AATGCTGCTGCAGAATCAAGATGCTTATTTAAAAGTAGCAGGTGGAGTGAAATTAGATGAACCAGCAATCGATTTAGCTG
TAGCCGTTAGCATTGCTTCAAGCTTCAGAGATGCTGCTTCTAGCGCTACAGATGTTATTGTAGGAGAGGTAGGATTAACA
GGCGAAGTACGCCGAGTGTCTAGAATTGAACAGCGCGTTCAAGAAGCGGTAAAATTAGGGTTCCAACGAATTATTATTCC
GGAGAAAAATTTAGGAGGATGGAAAGTCCCAGATGGTATTGATGTGATTGGGGTATCAACAGTAGCGGAGGCTCTGCAAT
ATACATTAGGAGGATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(68-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

76.035

100

0.762
  radA Streptococcus pneumoniae Rx1

63.135

98.908

0.624
  radA Streptococcus pneumoniae D39

63.135

98.908

0.624
  radA Streptococcus pneumoniae R6

63.135

98.908

0.624
  radA Streptococcus pneumoniae TIGR4

63.135

98.908

0.624
  radA Streptococcus mitis NCTC 12261

63.135

98.908

0.624
  radA Streptococcus mitis SK321

63.135

98.908

0.624