Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   I5R27_RS20155 Genome accession   NZ_CP065202
Coordinates   4431667..4433034 (-) Length   455 a.a.
NCBI ID   WP_083369766.1    Uniprot ID   -
Organism   Pseudomonas fragi strain A13BB     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4426667..4438034
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I5R27_RS20135 (I5R27_20130) - 4427485..4428039 (+) 555 WP_083369768.1 hypothetical protein -
  I5R27_RS20140 (I5R27_20135) yjiA 4428136..4429104 (-) 969 WP_086795839.1 GTPase -
  I5R27_RS20145 (I5R27_20140) - 4429251..4429448 (-) 198 WP_007937254.1 YbdD/YjiX family protein -
  I5R27_RS20150 (I5R27_20145) - 4429464..4431530 (-) 2067 WP_083369767.1 carbon starvation CstA family protein -
  I5R27_RS20155 (I5R27_20150) radA 4431667..4433034 (-) 1368 WP_083369766.1 DNA repair protein RadA Machinery gene
  I5R27_RS20160 (I5R27_20155) - 4433080..4433610 (-) 531 WP_086795838.1 ankyrin repeat domain-containing protein -
  I5R27_RS20165 (I5R27_20160) katB 4433746..4435287 (-) 1542 WP_016779364.1 catalase KatB -
  I5R27_RS20170 (I5R27_20165) mscL 4435554..4435967 (+) 414 WP_016779363.1 large-conductance mechanosensitive channel protein MscL -
  I5R27_RS20175 (I5R27_20170) - 4436038..4436814 (-) 777 WP_169845862.1 ferredoxin--NADP reductase -
  I5R27_RS20180 (I5R27_20175) - 4437188..4437901 (+) 714 WP_086795837.1 helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 48755.36 Da        Isoelectric Point: 7.1315

>NTDB_id=507963 I5R27_RS20155 WP_083369766.1 4431667..4433034(-) (radA) [Pseudomonas fragi strain A13BB]
MAKPKRIYGCTECGATFPKWAGQCGECGVWNTLVETIVESGAAAPPSGRTGWTGQQAQLKTLAEVSIEELPRFSTASGEL
DRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCQMATRMPALYVTGEESQQQVAMRARRLGLPQDQLKVMTETCIESIIA
TAKLEKPKVMVIDSIQTIFTEQLQSAPGGVSQVRESAALLVRYAKSSGTAIFLVGHVTKEGALAGPRVLEHMVDTVLYFE
GESDGRLRLLRAVKNRFGAVNELGVFGMTDKGLKEVSNPSAIFLTRAQEEVPGSVVMATWEGTRPMLVEVQALVDDSHMS
NPRRVTLGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVGGVKVLETASDLALMAAVMSSLRNRPLPHDLLVFGEVGLSG
EVRPVPSGQERLKEAAKHGFKRAIVPKGNAPKESPPGLKVIGVTRLEQALDALFE

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=507963 I5R27_RS20155 WP_083369766.1 4431667..4433034(-) (radA) [Pseudomonas fragi strain A13BB]
ATGGCCAAGCCCAAGCGCATTTACGGCTGCACCGAGTGCGGCGCAACGTTTCCCAAGTGGGCCGGGCAGTGCGGCGAGTG
CGGCGTCTGGAACACCCTGGTCGAAACCATCGTTGAAAGCGGGGCTGCCGCGCCCCCCAGCGGCCGTACCGGCTGGACCG
GCCAACAGGCCCAGCTCAAGACCCTGGCCGAAGTCAGCATTGAAGAGTTGCCGCGCTTTTCCACGGCCTCCGGCGAGCTG
GACCGGGTGCTCGGTGGCGGGCTGGTGGATGGCTCGGTGGTATTGATCGGCGGCGACCCTGGGATCGGCAAATCCACCAT
CCTGCTGCAAACCCTTTGTCAGATGGCCACCCGCATGCCGGCGCTGTACGTCACCGGCGAGGAATCCCAGCAACAAGTGG
CCATGCGCGCCCGGCGCCTGGGGTTGCCCCAGGATCAGCTCAAGGTAATGACCGAGACCTGTATCGAAAGCATCATCGCC
ACCGCCAAGCTCGAAAAGCCCAAGGTCATGGTGATTGACTCGATCCAGACCATCTTTACCGAGCAGCTGCAATCGGCCCC
CGGCGGCGTGTCGCAAGTGCGCGAAAGTGCGGCCTTGCTGGTGCGATATGCGAAATCCAGCGGTACGGCGATTTTCCTGG
TGGGCCATGTGACCAAAGAAGGCGCACTGGCCGGGCCGCGGGTGCTGGAGCATATGGTGGATACCGTGCTGTATTTCGAG
GGCGAGTCCGACGGGCGCCTGCGTTTGTTGCGAGCGGTAAAAAACCGTTTTGGCGCGGTCAACGAGCTGGGTGTGTTCGG
CATGACCGATAAGGGCCTGAAAGAAGTCTCCAACCCTTCTGCAATTTTTCTTACCCGTGCTCAGGAAGAAGTCCCGGGCA
GCGTGGTGATGGCCACCTGGGAAGGCACCCGTCCGATGCTGGTGGAGGTGCAGGCGTTGGTGGACGACAGTCATATGTCC
AACCCGCGCCGCGTTACCTTGGGCCTGGACCAGAATCGTCTGGCCATGTTGTTGGCCGTCTTGCATCGCCACGGCGGTAT
TCCTACCCATGATCAGGATGTGTTCCTCAACGTGGTGGGCGGGGTCAAGGTGCTGGAAACCGCCTCTGACCTGGCGCTGA
TGGCCGCCGTGATGTCCAGCTTGCGCAACCGTCCGCTGCCTCACGACCTGCTGGTGTTCGGTGAAGTCGGCCTGTCGGGC
GAAGTGCGTCCGGTGCCAAGCGGTCAGGAGCGGCTCAAGGAAGCGGCCAAGCACGGCTTCAAGCGTGCCATCGTGCCCAA
GGGCAACGCCCCCAAGGAATCGCCGCCGGGCCTGAAGGTGATTGGCGTGACCCGTCTGGAACAGGCGCTGGATGCGTTGT
TCGAGTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(353-429)

AAA domain (AAA_25)

(81-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.123

100

0.492
  radA Streptococcus mitis SK321

47.368

100

0.475
  radA Streptococcus mitis NCTC 12261

47.149

100

0.473
  radA Streptococcus pneumoniae Rx1

47.149

100

0.473
  radA Streptococcus pneumoniae D39

47.149

100

0.473
  radA Streptococcus pneumoniae R6

47.149

100

0.473
  radA Streptococcus pneumoniae TIGR4

47.149

100

0.473